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EC number: 480-240-4 | CAS number: 185257-07-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Additional information
There are reliable in vitro studies available to assess the potential of the test substance for both gene mutations in bacteria and cytogenicity in mammalian cells.
Gene mutation in bacteria
In a GLP conform study according to OECD guideline 471, the substance MGDN (purity: 97.9 %) was tested for its mutagenic potential based on the ability to induce point mutations in selected loci of several bacterial strains, i.e. Salmonella typhimurium TA 1535, TA 100, TA 1537, TA 98 and Escherichia coli WP2 uvrA (BASF 1998). Two independent assays were performed, a standard plate test (SPT) and a preincubation test (PIT), both with a dose range of 20 µg - 5000 µg/plate, and both with and without metabolic activation (Aroclor 1254-induced rat liver S-9 mix).
An increase in the number of his+ or trp+ revertants was not observed in the standard plate test or in the preincubation test either without S-9 mix or after the addition of a metabolizing system. No precipitation of the test substance was found. A slight decrease in the number of revertants was occasionally observed.
According to the results of the present study, the test substance MGDN is not mutagenic in the Salmonella typhimurium/ Escherichia coli reverse mutation assay under the experimental conditions chosen here.
Gene mutation in mammalian cells
no data
Cytogenicity in mammalian cells
In a GLP conform study according to OECD guideline 473, the substance MGDN (purity: 99.9 weight-%) was assessed for its potential to induce structural chromosomal aberrations (clastogenic activity) and/or changes in the number of chromosomes (aneugenic activity) in V79 cells in vitro both in the presence and in the absence of a metabolizing system.
According to an initial range-finding cytotoxicity test for the determination of the experimental doses (the test substance did not exhibit any pronounced toxicity up to the highest recommended doses, i.e. 1 500 μg/mL (ca. 10 mM), the following doses were tested (the test groups in brackets were not evaluated):
- 1st experiment:
4-hour exposure, 18-hour sampling time, without S-9 mix: 0; (187.5) ; 375.0; 750.0; 1500 µg/mL;
4-hour exposure, 18-hour sampling time, with S-9 mix: 0; (187.5) ; 375.0; 750.0; 1500 µg/mL
- 2nd experiment (confirmation of the results of the 1st experiment including continuous exposure and a second sampling time)
18-hour exposure, 18-hour sampling time, without S-9 mix: 0; (187.5) ; 375.0; 750.0; 1500 µg/mL
18-hour exposure, 28-hour sampling time, without S-9 mix: 0; (375.0; 750.0;) 1500 µg/mL
4-hour exposure, 28-hour sampling time, with S-9 mix: 0; (187.5) ; 375.0; 750.0; 1500 µg/mL
About 2- 3 hours prior to harvesting the cells, Colcemid was added to arrest cells at a metaphase-like stage of mitosis (c-metaphases). After preparation of the chromosomes and staining with Giemsa, 100 metaphases for each culture in the case of the test substance, and vehicle controls, or 50 cells for each culture in the case of the concurrent positive controls, were analyzed for chromosomal aberrations.
The negative controls (vehicle controls) gave frequencies of aberrations within the range expected for the V79 cell line. Both of the positive control chemicals, i.e. EMS and cyclophosphamide, led to the expected increase in the number of cells containing structural chromosomal aberrations.
On the basis of the results of the present study, the test substance did not cause any increase in the number of structurally aberrant metaphases incl. and excl. gaps at both sampling times either without S-9 mix and/or after adding a metabolizing system in two experiments performed independently of each other. No increase in the frequency of cells containing numerical aberrations was demonstrated either. Thus, under the experimental conditions of this assay, MGDN is considered not to be a chromosome-damaging (clastogenic) agent under in vitro conditions in V79 cells.
Short description of key information:
in vitro
Gene mutation in bacteria
Ames test, S. typhimurium/ E.coli, with and without metabolic activation: negative (GLP, OECD 471; BASF AG 1998)
Gene mutation in mammalian cells
no data
Cytogenicity in mammalian cells
Chromosome aberration assay, V79 cells, with and without metabolic activation: negative (GLP; OECD 473; BASF AG 2006)
in vivo
no data
Endpoint Conclusion:
Justification for classification or non-classification
Based on the available in vitro studies, there is no indication for a mutagenic potential of the test substance.
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