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EC number: 208-051-2 | CAS number: 506-68-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mixture
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- mitomycin C
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 102
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Conclusions:
- From the overall results, it was concluded that Cyanogen Bromide did not induce any mutation and considered as NON-MUTAGENIC upto the concentration of 4.88 μg/plate, under the test conditions employed.
- Executive summary:
The mutagenic effect of Cyanogen Bromide Sponsored by Libor Bachura vyzkum a vyroba, Czech Republic, was evaluated by Ames Salmonella typhimurium - Reverse mutation assay. This study was conducted as per the OECD Guideline No. 471, Adopted 21st July, 1997 at Bioscience Research Foundation, India.
The five tester strains (TA98, TA100, TA102, TA1535 and TA1537) were assessed to be intact. The test substance was dissolved in Distilled water. During precipitation test, no precipitation was observed even at the highest concentration of 5000 μg / plate in the top agar mix.
In the range finding study, two tester strains TA98 and TA100 was selected for cytotoxic analysis. Preincubation method was followed and seven test concentrations along with the solvent control (Distilled water) were treated both in the presence and absence of metabolic activation (S9 mix). Based on the precipitation test, the following concentrations were selected viz., 78.13, 156.25, 312.5, 625, 1250, 2500 and 5000 μg / plate (separated by factor 2 spacing). There was no contamination found in the control and the treated test concentrations. Duplicate culture was set for all test concentrations. Intact background lawn was observed in the concentration of 78.13 μg/plate in +S9 and –S9 of both TA 98 and TA 100 strains. The background lawn of the controls of both tester strains were found to be intact. In TA 98 Extreme inhibition was observed in test concentration 625, 1250, 2500 and 5000 μg/plate in both +S9 and –S9. Moreover, TA 100 extreme inhibition was observed test concentrations 312.5, 625, 1250, 2500 and 5000 μg/plate in both the +S9 and –S9. The revertant colonies of cultures were not comparable to the solvent control in both the strains with the presence and absence of S9 mix.
Based on the range finding results, the mutagenic effect was tested in the main study. Five tester strains (TA98, TA100, TA102, TA1535 and TA1537) were tested in preincubation method followed by plate incorporation method. Five concentrations 4.88, 9.76, 19.53, 39.06 and 78.13μg/plate in the presence and absence of S9 mix along with the solvent and concurrent positive controls were treated. Triplicate cultures were set for all the test concentrations.
There was no contamination found in the controls and the test concentrations treated. Intact background lawn was observed in all the test concentrations and the control plates in the presence and absence of S9 mix in all the five tester strains. The revertant colonies of the solvent controls in all the tester strains are comparable to the historical control values. In the treated cultures, the revertant colonies are comparable to the solvent control in all the strains except TA 102 in which concentration related increase in the revertant colonies was observed at the concentration of 9.76 and 19.53 μg/plate. On the contrary, 4.88 μg/plate was comparable with that solvent control. The results obtained from preincubation method were identical in plate incorporation method. The strain specific positive controls with the presence and absence of S9 mix exhibited multifold increase of the revertant colonies over the solvent control tested in both the preincubation and plate incorporation method.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Justification for classification or non-classification
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