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EC number: 212-850-1 | CAS number: 873-74-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January - March 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.3 (Acute Toxicity (Dermal))
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 4-aminobenzonitrile
- EC Number:
- 212-850-1
- EC Name:
- 4-aminobenzonitrile
- Cas Number:
- 873-74-5
- Molecular formula:
- C7H6N2
- IUPAC Name:
- 4-aminobenzonitrile
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- - Name of test material (as cited in study report): 4-Aminobenzonitril
- Physical state: solid
- Stability under test conditions: not stated
- Storage condition of test material: room temperature
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Crj: CD(SD)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Deutschland GmbH, 97633 Sulzfeld, Germany
- Age at study initiation: Approximately 8 weeks (males) and 12 weeks (females) at the time of administration.
- Weight at study initiation: 231 - 291 g
- Housing:Single caging in Makrolon cages type III (37.5 cm x 21.5 cm bottom area , 18 cm height). Wire mesh lids, Sanitation of cages once a week.
- Diet (e.g. ad libitum): Ssniff R/M-H maintenance diet for rats and mice (item V1534-300) ad libitum, supplied by Ssniff Spezialdiäten GmbH, 59494 Soest, Germany. Analysis of the feed for ingredients and contaminants is performed randomly by Ssniff.
- Water (e.g. ad libitum): Tap water, from an automatic watering system, ad libitum. Random samples of the water are analysed by the "AGES" 1226 Vienna, Austria, to check, if the water fulfils the requirements for drinking water for humans.
- Acclimation period: At least 7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): Mean of 20.41 °C in the dose range finding study, mean of 19.54 °C in the main study, (continuous control and recording).
- Humidity (%): Mean of 50.53 % in the dose range finding study, mean of 39.13 % in the main study, (continuous control and recording).
- Air changes (per hr): 12 per hour.
- Photoperiod (hrs dark / hrs light): Artificial light from 6 a.m. to 6 p.m.
Administration / exposure
- Type of coverage:
- semiocclusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: dorsal thoracic region, an area of 6.5 cm x 8 cm (52 cm²) was marked on a relaxed animal.
- % coverage: 10 %
- Type of wrap if used: A cellulose patch (Pehazell, Hartmann AG) with the calculated amount of the test substance on the surface and soaked with deionised water to get optimal contact with the skin, was applied to the test site and held in place by fixing marginally with non irritating tape (Blanderm Wundpflaster, 3M). The test site was covered by a semi-occlusive dressing (Fixomull Stretch, Fa. Beiersdorf).
REMOVAL OF TEST SUBSTANCE
- Washing (if done): Residual test substance was wiped off using wet cellulose tissue, if necessary.
- Time after start of exposure: 24 hours - Duration of exposure:
- 24 hours
- Doses:
- Range Finding Study: 400, 894 and 2000 mg/kg bw
Main Study: 2000 mg/kg bw - No. of animals per sex per dose:
- Range Finding Study: One male and one female per dose.
Main Study: Five male and five female per dose. (Both animals dosed with 2000 mg/kg bw in the preliminary test were included into the main study). - Control animals:
- not required
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Body weights were determined before administration, 7 and 14 days after the administration.
- Necropsy of survivors performed: yes
- Other examinations performed: Observations were performed 0 - 0.5, > 0.5 -1, > 1 - 2, > 2 - 4 and > 4 - 6 hours after administration of the test substance and then at least once a day for a total of 2 weeks. Observations included but were not limited to changes in skin, fur, eyes, the occurrence of secretions and excretions, autonomic activity, changes in gait, posture and the presence of convulsions. No skin examination of the administration site was possible during the exposure period while it was covered by the patch and wrappings. - Statistics:
- not applicable
Results and discussion
- Preliminary study:
- In a range finding study three groups of one male and one female each were dosed with 400, 894 or 2000 mg per kg body weight. All animals survived for 7 days after administration. Therefore a limit-test with one dose of 2000 mg per kg body weight was performed.
Both animals dosed with 2000 mg/kg in the preliminary test, were included into the main study.
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- All animals survived until the scheduled termination of the study.
- Clinical signs:
- other: General findings: All animals did not show any clinical signs during the entire observation period. Observations of skin conditions: Exposed skin was not found to be altered by the test substance.
- Gross pathology:
- No abnormal findings were made in the animals at terminal necropsy.
- Other findings:
- No abnormal findings.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- No local or systemic test substance related effects were noted from clinical observations or post-mortem examination at a limit dose of 2000 mg 4-Aminobenzonitrile per kg body weight and no mortality occurred.
- Executive summary:
In an acute dermal toxicity study according to OECD 402 ("Acute Dermal Toxicity") the test substance 4-Aminobenzonitrile was administered once topically on an area of approximately 6.5 cm x 8 cm on the dorsal thoracic region of five males and five female Spargue Dawley rats. The dose was 2000 mg per kg body weight. The test site was covered by a semi-occlusive dressing. The duration of the exposure was 24 hours.
No local or systemic toxic effects realted to the administration of 4-Aminobenzonitrile were noted from clinical observations or post-mortem examination at a limit dose of 2000 mg of the test substance per kg body weight and no mortality occurred.
Therefore the LD50(dermal) of 4-Aminobenzonitrile is > 2000 mg/kg body weigtht.
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