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Diss Factsheets
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EC number: 239-806-4 | CAS number: 15717-25-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP - Guideline study with acceptable restrictions Data on pH and temperature of the test solutions and reference substance (concentrations, EC50) are missing. No analytics were conducted.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Potassium 3-sulphonatopropyl acrylate
- EC Number:
- 250-465-0
- EC Name:
- Potassium 3-sulphonatopropyl acrylate
- Cas Number:
- 31098-20-1
- Molecular formula:
- C6H10O5S.K
- IUPAC Name:
- potassium 3-(acryloyloxy)propane-1-sulfonate
- Details on test material:
- - Name of test material (as cited in study report): Kalium-3-sulfonatopropylacrylat
- Physical state: solid
- Analytical purity: no data
- Lot/batch No.: ss 32799-465
- Storage condition of test material: in a refrigerator at 5 - 10°C
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution was prepared one day before test start by adding 125 mg test substance to 800 mL destilled water and 24 h treatment with a magnetic stirrer. Afterwards 200 mL distilled water were added. Each test concentration is 200 mL, consisting of stock solution, 20 mL nutrient solution, 20 mL inoculum and bidestilled water if dilution is necessary. The final volume of each test concentration is 200 mL.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: chordat 86.81
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: A 7 day old strain is diluted with aqua bidest to a cell concentration of 10E5 cells/mL and than filled up to 1 L with 100 mL nutrient solution and deionised water. 50 mL of this solution is filled into 300 mL Erlenmeyer flasks and put into a breeding room at 23 ± 2°C for 3 days. The flasks are homogenised by daily swaying per hand. The different precultures were united again after 3 days. For inoculum the algae were diluted to 10E5 cell/mL again.
ACCLIMATION
- Culturing media and conditions: same as test
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 23 ± 2 °C
- pH:
- no data
- Nominal and measured concentrations:
- nominal: 0, 1, 5, 10, 25, 50, 75, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Type: closed with steel caps
- Size, fill volume: 300 mL flasks filled with 50 mL test solution
- Aeration: none
- Initial cells density: 10E5 cells/mL
- Control cells growth rate: 1.36
- No. of vessels per concentration (replicates): 4 replicates, 3 to measure cell density (via extinction during test period), 1 for measurement of extinction and pH at test start; furthermore 1 replicate with blank test concentrations (without inoculum)
- No. of vessels per control (replicates): 6
- No. of vessels per reference control (replicates): 4
- Homogenisation of algae suspension: by daily swaying per hand only once
- Growth medium: standard medium used
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: not stated
- Light intensity and quality: 85 µE/ m^2 s with neon tube (Osram L36W/ 25 universal white)
EFFECT PARAMETERS MEASURED: Cell density at test start and after 24, 48 and 72 h.
- Determination of cell concentrations: spectrophotometric (measurement of extinction at 578 nm) - Reference substance (positive control):
- yes
- Remarks:
- Potassiumdichromate (K2Cr2O7)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 25.72 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 19.408 - 33.836, taken from raw data
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.613 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 0.614 - 2.957, taken from raw data
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.39 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL: 1.813 - 7.395 taken from raw data
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.908 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks on result:
- other: 95% CL: 0.042 - 2.063, taken from raw data
- Details on results:
- - Exponential growth in the control: yes, growth rate 1.36/d (corresponding to a 59.9-fold growth)
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes, inhibition of cell growth 62.4%.
Any other information on results incl. tables
Table 1: Inhibition of cell growth rate at different test concentrations within 72 h:
Test concentration [mg/L] | Inhibition of growth rate [%] |
1 | -2.02 |
5 | 25.45 |
10 | 32.56 |
25 | 51.88 |
50 | 62.36 |
75 | 71.1 |
100 | 68.02 |
Table 2: Inhibition of cell growth rate by the reference substance within 72 h:
Test concentration [mg/L] | Inhibition of growth rate [%] |
0.5 | 21.46 |
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.