Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 270-156-4 | CAS number: 68411-97-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A defined amount of the test substance (stock solution concentration = 500 mg/L) was weighed into a measuring flask and the total volume was filled up with dilution water. The test bottles were conditioned about 15 min before the test was started.
- Differential loading: yes
- Controls: yes, dilution water served as negative control - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Length at study initiation: 2.0 cm (average length)
ACCLIMATION
- Acclimation period: at least 14 d
- Feeding frequency during acclimation: daily
FEEDING DURING TEST
- Frequency: no feeding during the test - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 0 h: 18.2 (control), 18.2 - 18.3 °C (treatments)
96 h: 18.2 (control), 18.1 - 18.2 °C (treatments) - pH:
- At 0 h: 7.8 (control), 7.6 - 7.8 (test solutions)
At 96 h: 7.7 (control), 7.7 - 7.8 (test solutions) - Dissolved oxygen:
- At 0 h: 8.1 mg/L (control), 7.4 - 7.9 mg/L (test solutions)
At 96 h: 8.1 mg/L (control), 6.9 - 7.5 mg/L (test solutions), corresponding to 86.5% of the air saturation - Nominal and measured concentrations:
- nominal: control, 1.0, 2.0, 5.0, 10.0 and 20.0 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers
- fill volume: 7 L
- Aeration: yes, constantly
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: chlorine-free drinking water
- Hardness (dilution water): 37.4 mg/L Ca-content (between 4 - 100 mg/L)
- Copper content: < 0.01 mg/L
- Chlorine: none
- Culture medium different from test medium: no
- Intervals of water quality measurement: PH values, O2 concentration and temperature were measured at 0, 24, 48, 72 and 96 h
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 h light-dark cycle
- Light intensity: indirect day-similar light
EFFECT PARAMETERS MEASURED:
The fish were inspected at 24, 48, 72 and 96 h.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - 2.5
- Range finding study: no - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 3.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC10
- Effect conc.:
- 1.76 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: 0%
- Effect concentrations exceeding solubility of substance in test medium: ?? - Reported statistics and error estimates:
- The statistical evaluation is based on a Probit analysis. The calculation of the Probit values with the date after 24 to 96 h bases was performed with with computer program "Dosis 50". In this program the experimentally determined percentages in the range from 1 - 99% can be used for the calculation of the Probit values and so for the evaluation of the effective concentrations. For the experimental values of 0% and 100% there are no Probit values defined. For the calculation of the Probit values of these percentages the percentages 1% and 99% were used.
- Sublethal observations / clinical signs:
The highest tested concentration of test substance producing no mortality under test conditions after 96 h was 1.0 mg/L.
The lowest tested concentration of test substance producing 100% mortality under test conditions after 96 h was 10.0 mg/L.
Table 1. Mortality in 24 h intervals.
Concentration of test substance
[mg/L]
Dead fish [%] after 24 h
Dead fish [%] after 48 h
Dead fish [%] after 72 h
Dead fish [%] after 96 h
20.0
100
100
100
100
10.0
71
100
100
100
5.0
29
71
71
86
2.0
14
14
14
14
1.0
0
0
0
0
control
0
0
0
0
- Validity criteria fulfilled:
- yes
- Remarks:
- No analytical measurement was conducted. Thus, the deviation from the nominal concentration could not be checked.
Reference
Description of key information
LC50 (96 h) = 3.16 mg/L test substance (nominal, Danio rerio, OECD 203)
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 3.16 mg/L
Additional information
One GLP-study according to OECD 203 investigating the short-term toxicity of Glycine, N-methyl-, N-coco acyl derivatives (CAS 68411-97-2) to freshwater fish is available. The test organism Danio rerio was exposed to five nominal concentrations of the test substance ranging from 1.0 - 20.0 mg/L (1, 2, 5, 10 and 20 mg/L) in controlled conditions for 96 h. No analytical measurement was conducted. Mortality was recorded at daily intervals and was compared to the negative control at test termination. After 96 h mortality was observed at concentrations ≥ 2.0 mg/L. The derived 96 h nominal LC50 value was 3.16 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)