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Diss Factsheets
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EC number: 227-774-4 | CAS number: 5977-14-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1974
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Study conducted prior to GLP and OECD guideline implementation.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 974
- Report date:
- 1974
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Version / remarks:
- DOT (Transport-Classification) Guideline
- Deviations:
- not specified
- GLP compliance:
- no
- Remarks:
- Study conducted prior to GLP and OECD guideline implementation.
Test material
- Reference substance name:
- Acetoacetamide
- EC Number:
- 227-774-4
- EC Name:
- Acetoacetamide
- Cas Number:
- 5977-14-0
- Molecular formula:
- C4H7NO2
- IUPAC Name:
- acetoacetamide
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- not available
Test animals
- Species:
- rabbit
- Strain:
- not specified
- Details on test animals or test system and environmental conditions:
- albino rabbits
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- abraded
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Amount / concentration applied:
- 0.5 g
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 48 hours after initial application
- Number of animals:
- 6
- Details on study design:
- The intact and abraded skin of 6 albino rabbits was employed for this study. The hair was clipped from the backs with the aid of angora clippers. Four areas of the back, placed approximately ten centimeters apart, were designated for the patches. Areas 2 and 3 were abraded by making four epidermal incisions (two perpendicular to two others in the area of the patch). The patches consisted of 1.5 inch x 1.5 inch 12 ply gauze squares. The patches were secured to the area by thin bands of adhesive tape. The material to be tested (0.5 grams) was introduced beneath the patch. The entire trunks of the animlas were then wrapped in claear plastic trunk bands. The trunk bands help to hold the patches in position and retard evaporation of volatile substances during the four hours exposure period. Upon removal of the patches the resulting reactions were evaluated on basis of weighted scores.
Following this initial reading, all test sites were washed with appropriate solvent to prevent further exposure. Readings were again made at 24 and 48 hours after the iniitial application. Each test substance is evaluated on a total of site (6 abraded and 6 intact)
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- animal: 1-6
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks:
- no signs of irritation (erythema) at any time
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- edema score
- Basis:
- animal: 1-6
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks:
- no signs of irritation (erythema) at any time
- Remarks on result:
- no indication of irritation
- Other effects:
- No other effects reported.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Based on the findings, Acetoacet Amide is not irritating to the rabbit skin.
- Executive summary:
6 albino rabbits were tested for skin irritation with Acetoacet Amide according to a company guideline similar to OECD 404. Following this guideline 0.5 g of the test item were tested at the intact and abraded skin under occlusive coverage.
The exposure period was 4h. After removal of the patch the remnant test item was removed with appropriate solvent. The observation period was 48 hours after initial application.
None of the animals showed any signs of skin irritation or corrosion at any time.
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