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EC number: 685-521-7 | CAS number: 98796-53-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- For details and justification of read-across please refer to the attached report in section 13 of IUCLID.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Filtrate of nominal 100 mg test item/L
- Basis for effect:
- growth rate
- Remarks on result:
- not determinable because of methodological limitations
- Remarks:
- Concentration must be below LOD of 0.01 mg/L though a supersatured solution of the test item was prepared.
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Filtrate of nominal 100 mg test item/L
- Basis for effect:
- growth rate
- Remarks on result:
- not determinable because of methodological limitations
- Remarks:
- Concentration must be below LOD of 0.01 mg/L though a supersatured solution of the test item was prepared.
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Filtrate of nominal 100 mg test item/L
- Basis for effect:
- growth rate
- Remarks on result:
- not determinable because of methodological limitations
- Remarks:
- Concentration must be below LOD of 0.01 mg/L though a supersatured solution of the test item was prepared.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Filtrate of nominal 100 mg test item/L
- Basis for effect:
- other: Yield
- Remarks on result:
- not determinable because of methodological limitations
- Remarks:
- Concentration must be below LOD of 0.01 mg/L though a supersatured solution of the test item was prepared.
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Filtrate of nominal 100 mg test item/L
- Basis for effect:
- other: Yield
- Remarks on result:
- not determinable because of methodological limitations
- Remarks:
- Concentration must be below LOD of 0.01 mg/L though a supersatured solution of the test item was prepared.
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- Filtrate of nominal 100 mg test item/L
- Basis for effect:
- other: Yield
- Remarks on result:
- not determinable because of methodological limitations
- Remarks:
- Concentration must be below LOD of 0.01 mg/L though a supersatured solution of the test item was prepared.
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): No. The microscopic examination of the shape of the algal cells after 72 hours of test duration did not show any difference between the algae that had been growing up to a filtrate of 100 mg test item/L and the algal cells in the control. Thus, the shape of the algal cells was not obviously affected up to the highest concentration tested.
- Unusual cell shape: No
- Colour differences: No
- Flocculation: No
- Adherence to test vessels: No
- Aggregation of algal cells: No
- Any stimulation of growth found in any treatment: No
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The water solubility of test item was indicated to be low (< 0.51 mg/L) and when preparing the fresh media, particles were floating on the surface or were lying on the bottom of the flask. Therefore, a filtrate of 100 mg test item/L representing the highest test concentration and dilutions of 1:3, 1:9, 1:27 and 1:81 of this filtrate, and a control were tested. The preparation of the test solution was conducted according to the OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures (OECD 23). - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- ErC50: 0.963 mg test item/L (95 % C.I.: 0.927 - 1.001 mg test item/L) - Reported statistics and error estimates:
- Based on the calculated cell densities, the 72 hours ErC50 and the 72 hours EyC50 could not be calculated by a statistical analysis due to the lack of effects and were therefore determined directly from the raw data. For the determination of the 72 hours LOEC and the 72 hours NOEC, the calculated growth rates and yields at each test concentration were tested for significant differences compared to the control values by Dunnett's t- test.
The software used to perform the statistical analysis was ToxRat Professional, Version 3.2.1, ToxRat® Solutions GmbH. - Validity criteria fulfilled:
- yes
- Conclusions:
- No effect on the growth or yield of Pseudokirchneriella subcapitata was observed up to the limit of water solubility of the test substance.
The analytical verification could not determine a measured concentration as the concentration of the test item was too low. Although a saturated solution was prepared by stirring for 48 h. The substance is hardly soluble and the limit of detection was 0.01 mg/L. - Executive summary:
In a 72-hour acute toxicity study, the cultures of Pseudokirchneriella subcapitata (KORSHIKOV), Strain No. 61.81 SAG were exposed to 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxythymidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite] at nominal concentrations of 100, 33, 11, 3.7 and 1.2 mg test item/L under static conditions in accordance with the OECD guideline 201. No effect was observed up to the limit of water solubility of the test substance. The EC50, EC20 and EC10 for yield and growth rate were estimated to be greater than the highest test concentration of the filtrate of 100 mg test item/L, which, however, could not be quantified analytically as the content was below the limit of detection. The NOEC was determined to be at least the filtrate of 100 mg test item/L. The LOEC was estimated to be greater than the filtrate of 100 mg test item/L. Therefore, no toxicity towards the test organism up to the limit of solubility was detected for the test item. The analytical verification of the concentrations was below the LOD of 0.01 mg test item/L.
This toxicity study is classified as acceptable guideline requirements for Freshwater Alga and Cyanobacteria, Growth Inhibition Test with P. subcapitata.
Results Synopsis
Test Organism: of Pseudokirchneriella subcapitata (KORSHIKOV), Strain No. 61.81 SAG
Test Type (Flowthrough, Static, Static Renewal): Static
Parameter
Yield
[mg test item/L]Growth rate
[mg test item/L]72-hour EC50
>Filtrate of nominal 100 mg/L
>Filtrate of nominal 100 mg/L
95 % conf. interval
n.d.
n.d.
72-hour EC20
>Filtrate of nominal 100 mg/L
>Filtrate of nominal 100 mg/L
95 % conf. interval
n.d.
n.d.
72-hour EC10
>Filtrate of nominal 100 mg/L
>Filtrate of nominal 100 mg/L
95 % conf. interval
n.d.
n.d.
72-hour NOEC
≥Filtrate of nominal 100 mg/L
≥Filtrate of nominal 100 mg/L
72-hour LOEC
>Filtrate of nominal 100 mg/L
>Filtrate of nominal 100 mg/L
n.d. = not determinable
Endpoint(s) Effected: Growth rate, Yield
This information is used in a read-across approach in the assessment of the target substance.For details and justification of read-across please refer to the attached report in section 13 of IUCLID.
Reference
Biological results:
The EC50, EC20 and EC10 for yield and growth rate were estimated to be greater than the highest test concentration of the filtrate of 100 mg test item/L, which, however, could not be quantified analytically as the content was below the limit of detection. The NOEC was determined to be at least the filtrate of 100 mg test item/L. The LOEC was estimated to be greater than the filtrate of 100 mg test item/L. Therefore, no toxicity towards the test organism up to the limit of solubility was detected for the test item.
Analytical results:
The quantification of the test item 5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-deoxythymidine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite] in the test samples was performed using liquid chromatography with UV detection. The chosen analytical method was the most sensitive method applicable. The concentrations of the test item were analysed in the duplicate test media samples from all test concentrations (filtrate of the test item stock solution and its dilutions) and the duplicate control samples from all sampling times. No substance was detected in any sample (see Table 1), concluding that the maximum soluble amount of the test item must be below the Limit of Detection (LOD) of the method (0.01 mg test item/L).
Validity Criteria of the Study |
|
Cell Density |
|
Coefficient of Variation of Sectional (Daily) Growth Rates in Control Cultures: |
|
Coefficient of Variation of Average Growth between Control Replicates: |
|
|
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Table 1. Summary of Analytical Results
1mean
value of all measured samples per treatment group
LOD: 0.01 mg/L |
Description of key information
No effect on the growth or yield of Pseudokirchneriella subcapitata was observed up to the limit of water solubility of the test substance in a growth inhibition tets conducted according to guideline 201 and GLP.
The analytical verification could not determine a measured concentration as the concentration of the test item was too low. Although a saturated solution was prepared by stirring for 48 h. The substance is hardly soluble and the limit of detection was 0.01 mg/L.
This information is used in a read-across approach in the assessment of the target substance. For details and justification of read-across please refer to the attached report in section 13 of IUCLID.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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