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EC number: 251-833-3 | CAS number: 34122-40-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- Version / remarks:
- 2006, corrected 2014
- GLP compliance:
- yes
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Batch No. 648300
- Purity & test date: 99.9%, Jul 26, 2017 - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage
- Storage conditions: The freshly obtained sludge was used immediately
- Concentration of sludge: The concentration of suspended solids was determined to be 4.9 g/L in the concentrated sludge. Before use, the sludge was allowed to settle (58 minutes) and the liquid was decanted for use as inoculum at the amount of 10 mL/L of mineral medium. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- IC (inorganic carbon)
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral medium
- Test temperature: 20 and 21°C
- pH: 7.4-7.8
- Continuous darkness: Incubation was conducted in the dark (on an orbital shaker)
TEST SYSTEM
- Culturing apparatus: Well sealed glass serum vessels
- Number of culture flasks/concentration: Triplicate test vessels for each treatment for each time interval. Five test vessels for analysis at the end of the test
- Method used to create aerobic conditions: Sealed vessels with a headspace of air
- Measuring equipment: Shimadzu TOC-VCPH total organic carbon analyser combined with a Shimadzu ASI-V autosampler.
SAMPLING
- Sampling frequency: At Day 1,7,14,21, and 28 for Inoculum Blank and Test Item groups. At Day 1,7,14 for Procedure Control and Toxicity Control groups. At Day 1 and 28 for Abiotic Control group
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
- Abiotic sterile control: Yes
- Toxicity control: Yes - Reference substance:
- benzoic acid, sodium salt
- Test performance:
- Acceptability of the Test
- the reference item was biodegraded by at least 60% (87%) within 14 days.
- the mean amount of TIC present in the inoculum blanks at the end of the test was < 3 mg C/L (1 mg C/L).
Since all criteria for acceptability of the test were met, this study was considered to be valid. - Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Assuming 100% mineralization of the test item to CO2, the Theoretical maximum IC production (ThIC) in excess of that produced in the inoculum blanks equals the Total Organic Carbon (TOC) added to each test vessel at the start of the test. The TOC content of TAOBN was determined to be 60.6%. Thus, the ThIC of TAOBN was calculated to be 0.61 mg/mg.
In the toxicity control more than 25% biodegradation occurred within 14 days (38%, based on ThIC). Therefore, the test item was assumed not to inhibit microbial activity. - Results with reference substance:
- The refence item achived 87% degradation in 14 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The test item was not readily biodegradable under the conditions of the Headspace Test presently performed. The test item was assumed not to inhibit microbial activity.
- Executive summary:
The objective of the study was to evaluate the organic test item for its ready biodegradability in an aerobic, aqueous inoculated medium. The test was performed in sealed vessels with a headspace of air.
The CO2 evolution resulting from the aerobic biodegradation of the test item was determined by measuring the inorganic carbon (IC) produced in the test vessels in excess of that produced in inoculum blank. Biodegradation was expressed as a percentage of the ThIC, based on the quantity of test item (as C) initially added. The relative biodegradation values calculated from the measurements performed during the test period revealed no biologically relevant biodegradation of the test item (0% after 28-days, based on the ThIC). In conclusion, the test item is designated as not readily biodegradable.
In the toxicity control more than 25% biodegradation occurred within 14 days and the reference substance achieved 87% degradation within 17 days. Therefore, the test item was assumed not to inhibit microbial activity and the test system was viable.
Reference
Description of key information
Not readily biodegradable, 0% degradation in 28 days under the conditions of OECD 310.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
The objective of the study was to evaluate the organic test item for its ready biodegradability in an aerobic, aqueous inoculated medium. The test was performed in sealed vessels with a headspace of air.
The CO2 evolution resulting from the aerobic biodegradation of the test item was determined by measuring the inorganic carbon (IC) produced in the test vessels in excess of that produced in inoculum blank. Biodegradation was expressed as a percentage of the ThIC, based on the quantity of test item (as C) initially added. The relative biodegradation values calculated from the measurements performed during the test period revealed no biologically relevant biodegradation of the test item (0% after 28-days, based on the ThIC). In conclusion, the test item is designated as not readily biodegradable.
In the toxicity control more than 25% biodegradation occurred within 14 days and the reference substance achieved 87% degradation within 17 days. Therefore, the test item was assumed not to inhibit microbial activity and the test system was viable.
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