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EC number: 254-991-1 | CAS number: 40594-65-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 20th to April 30th, 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 5-methyl-2-(isopropyl)cyclohexyl nicotinate
- EC Number:
- 254-991-1
- EC Name:
- 5-methyl-2-(isopropyl)cyclohexyl nicotinate
- Cas Number:
- 40594-65-8
- Molecular formula:
- C16H23NO2
- IUPAC Name:
- 5-methyl-2-(isopropyl)cyclohexyl nicotinate
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Camposampiero (PD) WT from the aerobic aeration re-circulation.
- Inoculum final concentration: suspended solids 27.6 mg/l, microbiological count UFC/l 6.0x10^7
- Microbiological count: colony counting is performed on agar at 36 °C and 22 °C in Petri dishes. The thermostatted agar is poured into each plate and allowed to solidify and the plates are incubated for 44 h (36 °C) and 68 h (22 °C).
- Suspended solids determination: determined by filtration on 0.45 μm pre-weighted filter of 5 ml of inoculum and then drying the pre-weighted filter at 105 °C by constant weight. The results are in mg/l from the ratio between the residue on initial volume.
- Preparation of inoculum for exposure: the inoculum was washed with mineral medium and kept in aeration for 5 days inside the mineral medium. - Duration of test (contact time):
- 28 d
- Details on study design:
- TEST CONDITIONS
- Composition of medium: in a 50 l container 500 ml of a) stock solution were added to 20 l of water, then 50 ml of b) c) d) stock solutions were added; the volume is then adjusted to 50 litre. Stock solution a): In a 1 l flask were weighted and then adjusted to the 1 l volume with water: 8.5 g of potassium dihydrogen orthophosphate (KH2PO4), 21.75 g dipotassium hydrogen orthophosphate (K2HPO4), 33.4 g disodium hydrogen orthophosphate dihydrate (Na2HPO4. 2H2O), 0.5 g ammonium chloride (NH4Cl), pH 7.4. Stock solution b): in one 1 l flask were weighted and then adjusted to the 1 l volume with water: 36.4 g calcium chloride dihydrate (CaCl2. 2H20), Stock solution c): in a 1 l matrass were weighted and then adjusted to the 1 l volume with water: 22.5 g magnesium sulphate eptahydrate (MgSO4.7H20). Stock solution d): in a 1 l matrass were weighted and then adjusted to the 1 l volume with water: 0.25 g iron (III) chloride hexahydrate (FeCl3. 6H20) with water and mixed.
- Test temperature: 20-24 °C
- Sample solutions at the end of the test: pHstart= 7.4 pHend = 7.3 B - pHstart= 7.4 pHend = 7.2
- Aeration of dilution water: the system was aerated in continuous with CO2 free air with a flux between 30-100 ml/min.
TEST SYSTEM
- Culturing apparatus: bottles of 5-l volume, equipped with an aeration tube.
- Number of culture flasks/concentration: 1. Bottle 1 e 2 containing test substance.
- Trap and measurement of CO2 and volatile organics if used: every single bottle was connected to 3 Drechsel laid out in line containing 100 ml of BaOH 0.0125 M Τhe CO2 produced is trapped in barium hydroxide. Quantitative determination of CO2 was obtained titrating exceeding Ba(OH)2 with HCl 0.05 M using phenolphthalein as indicator.
- Preparation of the test system: in every bottle 3 litres of mineral medium were loaded with 10 ml of activated sludge and aerated for 24 hr with CO2 free air to reach suspended solids less than 30 mg/l. After 24 hr the test sample and the reference compound were added to yield a final concentration of 10-20 mgTOC/l. Every bottle was adjusted to the final volume of 3 l with mineral medium.
SAMPLING
- Sampling frequency: during the first ten days titrations were made every second or third day and then at least every fifth day.
CONTROL AND BLANK SYSTEM
- Inoculum blank: bottles 3 and 4 containing only inoculum
- Reference substance sterile control: bottles 5 and 6 containing the reference substance
- Toxicity control: bottle 7 containing test substance, reference compound and inoculum.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 76.7
- Sampling time:
- 28 d
- Details on results:
- Biodegradation of toxicity control (14 days) > 25 %
CO2 evolved from the blank is less than 70 mg/l
The difference of the results of replicate values is inferior to 20 % for the Test and Reference Substance.
BOD5 / COD results
- Results with reference substance:
- Biodegradation (28 days): 87.1 %
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Biodegradation (28 days) = 76.7 %
- Executive summary:
The biodegradation of the substance is evaluated according to the OECD Guideline 301B. A measured volume of inoculated mineral medium, containing a known concentration of the test substance as the nominal sole source of organic carbon is aerated by the passage of carbon dioxide-free air at a controlled rate. Degradation was followed over 28 days by determining the carbon dioxide produced. The CO2 is trapped in barium hydroxide and is measured by titration of the residual hydroxide. Toxicity, inocolum and reference control run in parallel. All validity criteria were met.
10 days immediately following the attainment of 10 % biodegradation the biodegradability of the test item is superior to the 60 % (10-d windows).
Biodegradation (28 days) = 76.7 %
The test material presents a 60 % CO2 production and this pass value is reached in a 10-d window within the 28-d period of the test.
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