N-(4-ethoxyphenyl)-3-hydroxy-4-[(2,4,5-trichlorophenyl)azo]naphthalene-2-carboxamide

Endpoint summary

Description of key information

Fish

In an acute toxicity test on the fish species Leuciscus idus similar to OECD 203 the submission substance proved to have no toxic or behavioural effects up to the highest tested concentration of 200 mg/L submission substance (500 mg/L test material).

LC50 (96h) > 200 mg/L (>500 mg/L test material)

LC0 (96h) = 200 mg/L (= 500 mg/L test material)

 

Daphnia

In the acute immobilisation test with Daphnia magna (STRAUS) the effect of the saturated solution# (100 mg/L) as limit concentration of the submission substance, was determined compliant to GLP according to OECD 202 (1999, draft) and EC Directive 92/69/EC Method C.2 (1992) (reliability category 1). The limit test was conducted under static conditions over 48 h. 20 test organisms were exposed to the limit concentration and control. No test item analysis was carried out. A reference test was carried out with potassium dichromate to determine the toxicity of the reference item. The EC50-value of the reference item of 2.07 mg/L after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/L according to AQS, DIN Guideline 38412 L 30. Water quality parameters pH-value and dissolved oxygen concentration, measured at 0 and 48 h, were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled. At the saturated solution (100 mg/L) of the test item no biologically significant effect was determined. Neither for the control nor for the treatment replicates any immobilized daphnids could be observed.

#) Saturated solution: The maximum dissolved concentration of the test item that can be achieved under the test conditions in the test medium, acc. to OECD Series, No. 23 (2000)6.

Alga

The toxicity of Pigment Red 112 to the unicellular freshwater green algae Desmodesmus subspicatus was determined according to the principles of OECD 201. In this study the test item was found not to inhibit the freshwater green algae Desmodesmus subspicatus after 72 h at the saturated solution (prepared with 1 mg/L). All effect levels are given based on the nominal concentration of Pigment Red 112.

The toxicity of Pigment Red 210 to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD 201 and compliant to GLP (reliability category 1). The aim of the study was to assess the effects on growth rate and yield over a period of 72 hours. Due to the test item being highly insoluble in water a saturated solution* with a nominal concentration of 1 mg/L was tested and no analytical determination was carried out since no suitable method for the determination of the test item could be established. In this study no inhibition on growth of the freshwater green alga Desmodesmus subspicatus was found after 72 hours exposure to the test item. The NOEC for inhibition of specific growth rate and yield after 72 hours was 1.00 mg/L.

Microorganisms

A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was carried out with Pigment red 112. The test was carried out under static conditions with the limit concentration 1000 mg/L. The respiration rates of the control, reference and test item replicates were measured after a contact time of three hours, and the inhibitory effects of the test and reference item were determined in comparison to the control respiration rates. No inhibition was observed for the test item.

A Respiration Inhibition Test with activated sludge according to OECD Guideline No. 209 was performed compliant to GLP with Pigment Red 146. The test was performed under static conditions with the nominal limit concentration of 1000 mg/L. The respiration rates of control, reference and test item concentrations were measured after a contact time of three hours, and the inhibitory effects of the test and reference concentrations were determined in comparison to the control respiration rates. No inhibition was observed at the tested limit concentration.