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Diss Factsheets
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EC number: 947-548-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 72 hours
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- Test conditions according to SOP E203 based on OECD Guideline for testing of chemicals, Freshwater Alga and Cyanobacteria, Growth Inhibition Test reference 201 (2011).
- Deviations:
- yes
- Remarks:
- Light intensity and temperature variations - not deemed to be significant enough to cause any effects.
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Name as supplied: ColaLiquid DC-5
Lot/Batch Number: 53467J16
Purity/Composition: CAS Number: 68855-69-6
Cocamide diisopropanolamine 84-88%
Glycerin 5-8%
Free Amine (DIPA) <5%
Chemex reference: ECO 161203 - Analytical monitoring:
- yes
- Details on sampling:
- Duplicate samples were taken at the start and end of the 72 hour exposure period. Samples were taken from remaining test media after filling test vessels for 0 hours and pooled replicate flasks for 72 hours.
- Vehicle:
- yes
- Remarks:
- nutrient growth medium
- Details on test solutions:
- Test concentrations: 0 (control), 0.32, 1.0, 3.2, 10, 32 and 100mg/l
Stock solutions were prepared for both the range-finding and definitive tests by addition of the test sample directly to nutrient growth medium in a volumetric flask of adequate volume. Calculated volumes of the stock solution were added to nutrient growth medium and made to volume to prepare the test concentrations and provide enough solution for testing and subsequent water quality measurements.
The test concentrations for both the range-finding test and definitive test were prepared separately by the addition of the test sample to nutrient growth medium in volumetric flasks of adequate volume to provide enough solution for testing and subsequent water quality measurements. - Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Species: Pseudokirchneriella subcapitata strain: CCAP 278/4 received 22 April 2016.
Source: Culture Collection of Algae and Protozoa SAMS Ltd Scottish Marine Institute, OBAN, Argyll PA37 1QA. Scotland, United Kingdom
Culture conditions: Temperature: 20.9 – 21.7C.
Illumination: 6030 - 8100 lux continuous white light. Orbiting: set to 200pm.
Culture media: Deionised water with added nutrients according to OECD 201 - Test type:
- other: Orbital shaking: 200rpm (Required: 200-250rpm)
- Water media type:
- other: nutrient growth medium
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Test temperature:
- Temperature range within incubator throughout test: 20.4 – 23.3°C
(Required: 21-24±2°C) - pH:
- Initial pH at 0 Hours: 7.48
pH range in control and test concentrations throughout test: 7.48 – 8.35 - Reference substance (positive control):
- yes
- Remarks:
- A separate reference study (ENV 11396) was run from 16 to 19 January 2017 using potassium dichromate (K1.0r2O7) as a means of checking the test procedure and sensitivity of the test species.
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: ErC50
- Effect conc.:
- 3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- other: EyC50
- Effect conc.:
- 1.37 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- other: growth rate and biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat. (dissolved fraction)
- Basis for effect:
- growth rate
- Details on results:
- The algal cells were examined microscopically during the determination of cell concentration. All cells within the control and test concentrations appeared normal, no abnormalities were observed
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 hour EC(r)50 and EC(y)50 of ColaLiquid DC-5 to Pseudokirchneriella subcapitata were 7.88mg/l and 2.33mg/l respectively.
The 72 hour EC(r)50 and EC(y)50 of ColaLiquid DC-5 to Pseudokirchneriella subcapitata were 3.0mg/l and 1.37mg/l respectively.
The 0 to 72-hour NOEC(r) was 1.0mg/l and the 0-72 hour LOEC was 3.2mg/l.
The algal cells were examined microscopically during the determination of cell concentration. All cells within the control and test concentrations appeared normal, no abnormalities were observed.
All validity criteria for the definitive test were met. The water quality measurements and incubation conditions were within accepted limits
Reference
Description of key information
The 48 hour EC(r)50 and EC(y)50 of ColaLiquid DC-5 to Pseudokirchneriella subcapitata were 7.88mg/l and 2.33mg/l respectively.
The 72 hour EC(r)50 and EC(y)50 of ColaLiquid DC-5 to Pseudokirchneriella subcapitata were 3.0mg/l and 1.37mg/l respectively.
The 0 to 72-hour NOEC(r) was 1.0mg/l and the 0-72 hour LOEC was 3.2mg/l.
The algal cells were examined microscopically during the determination of cell concentration. All cells within the control and test concentrations appeared normal, no abnormalities were observed.
All validity criteria for the definitive test were met. The water quality measurements and incubation conditions were within accepted limits
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1.37 mg/L
- EC10 or NOEC for freshwater algae:
- 1 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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