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EC number: 610-949-8 | CAS number: 53045-70-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12.12.2006-26.02.2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 1988
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- A stock solution with the concentration of 100 mg/L was prepared.
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Name and location of sewage treatment plant where inoculum was collected: sewage plant Darmstadt, Germany
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 18 - 20°C
- pH:
- 7.2 - 8.5
- Dissolved oxygen:
- 8.3 - 8.7 mg/L
- Nominal and measured concentrations:
- Nominal concentrations: 10, 32, 100, 320, 1000 mg/L
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol, 3.2, 10, 32 mg/L
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- ca. 108 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks on result:
- other: 95%CI: 7.0 - 254.5 mg/L
- Details on results:
- Based on inhibition rates, no EC50 could be calculated due to mathematical reasons. Since the maximum respiration inhibition was mesured to be about 50% at 1000 mg/L, the EC50 is proposed to be 1000 mg/L.
- Results with reference substance (positive control):
- 3h-EC20 3.2 mg/L
3h-EC50 9.7 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- The NOEC was determined to be 100 mg/L and the EC50 to be ca. 1000 mg/L.
- Executive summary:
Study design
A test on respiration inhibition of domestic sewage sludge was conducted according to OECD 209 and commission directive 88/302/EEC. The influence of the test item Art. 278718 (Ethyl-Butenol) on the activity of activated sludge was evaluated by measuring the respiration rate under defined conditions. The respiration rate (oxygen consumption) of an aerobic activated sludge fed with a standard amount of synthetic sewage was measured in the presence of various of 10, 32, 100, 320 and 1000 mg/L of the test item after an incubation period of 3 hours.
Results
In comparison to the inoculum controls the respiration rate of the activated sludge was slightly inhibited by about 8% at the lowest nominal test concentrations of 10 mg/L. At the next higher concentrations of nominal 32, 100, 320 and 1000 mg/L, a dose related inhibition of the respiration rate was found. At 1000 mg/L, an inhibition of about 51 % was measured. Concentrations exceeding 1000 mg/L nominal were not tested. Based on measured inhibition rates, no EC50 could be determined due to mathematical reasons. The EC20 was calculated to be 108 mg/L. The confidence limits were 7.0 mg/L and 254.5 mg/L for the lower and upper range respectively. Since the maximum respiration inhibition was measured to be about 50% at a test concentration of 1000 mg/L, the EC50 is proposed
to be 1000 mg/L. A NOEC can be established at 100 mg/L since the respiration inhibition was less than 20% at this concentration.Conclusion
After 3 hours of exposure, the NOEC was determined to be 100 mg/L and the EC50 to ca. 1000 mg/L.
Reference
Percentages of Inhibition:
Nominal concentration [mg/L] |
Inhibition [%] |
1000 |
51.4 |
320 |
24.3 |
100 |
18.9 |
32 |
13.5 |
10 |
8.1 |
Description of key information
The NOEC was determined to be 100 mg/L and the EC50 to be ca. 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
A test on respiration inhibition of domestic sewage sludge was conducted according to OECD 209. After 3 hours of exposure, the NOEC was determined to be 100 mg/L and the EC50 to be ca. 1000 mg/L.
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