Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 229-158-0 | CAS number: 6420-33-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 06 February 2017 to 08 March 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: in the main test controls and at 100 mg/L at 0 hour (bulk) and 48 hour (pooled from replicates) in duplicate
- Sample storage conditions before analysis:frozen - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION : A nominal amount of test item (100 mg) was dissolved in a 1000 mL volume of test water with the aid of sonication for approximately 5 minutes to give the 100 mg/L test concentration.
The prepared concentration was inverted several times to ensure adequate mixing and homogeneity. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Source: in-house laboratory culture
- Age : < 24 hours
- Feeding during test: none
ACCLIMATION
- Acclimation period: NA
- Culture conditions: temperature 18 to 22 °C; 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods.:
- Type and amount of food: algal suspension (Desmodesmus subspicatus) and Tetramin flake food suspensio
- Feeding frequency: daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- NA
- Test temperature:
- 19-22 ºC
- pH:
- 7.7-7.8
- Dissolved oxygen:
- 8.5-9.2 mg/L (102-108% of saturation)
- Salinity:
- NA
- Nominal and measured concentrations:
- nominal: 0 and 100 mg/L
measured (at 0 and 48 h):- Details on test conditions:
- TEST SYSTEM
- Test vessel: 150 mL glass beakers with 100 mL test preparation
- Type: closed
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: fresh water (Elendt M7 medium according to the guideline pH 7.9, kept at 21 ºC)
- Adjustment of pH: no
- Photoperiod: 16 h light
- Light intensity: 386 to 568 Lux
EFFECT PARAMETERS MEASURED: immobility:
RANGE-FINDING STUDY
- Test concentrations: 0, 0.1, 1, 10 and 100 mg/L
- Results used to determine the conditions for the definitive study:no immobilisation at any of the concentrations tested- Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: measured concentrations >80% of nominal
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: measured concentrations >80% of nominal
- Results with reference substance (positive control):
- potassium dichromate EC50 (48 h) 1.2 mg/L
- Reported statistics and error estimates:
- NA
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48 h EC50 for daphnia magna is >100 mg/L
- Executive summary:
In a static test according to OECD 202 20 daphnids/concentration were exposed to 0 and 100 mg/L in Elendt M7 medium. Measured concentrations at 0 and 100 mg/L were at 0 and 48 h: <LOQ and < LOQ, and 98.0 and 96.3 mg/L. No immobility was observed. The 48 h EC50 for daphnia magna is > 100 mg/L.
Reference
Description of key information
In a static test according to OECD 202 20 daphnids/concentration were exposed to 0 and 100 mg/L in Elendt M7 medium. Measured concentrations at 0 and 100 mg/L were at 0 and 48 h: <LOQ and < LOQ, and 98.0 and 96.3 mg/L. No immobility was observed. The 48 h EC50 for daphnia magna is > 100 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.