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EC number: 226-408-0 | CAS number: 5395-50-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: other routes
Administrative data
- Endpoint:
- acute toxicity: other routes
- Remarks:
- intraperitoneal
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1978-1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- - Principle of test: estimate of the potential acute hazard after a single administration (determination of the LD50)
- Short description of test conditions: intraperitoneal administration of a solution (test substance dissolved in aqua dest.)
- Parameters analysed / observed: LD50, Clinical Signs, Necropsy Findings, Pathology, Mortality, Mean Weight - GLP compliance:
- no
Test material
- Reference substance name:
- Tetrahydro-1,3,4,6-tetrakis(hydroxymethyl)imidazo[4,5-d]imidazole-2,5(1H,3H)-dione
- EC Number:
- 226-408-0
- EC Name:
- Tetrahydro-1,3,4,6-tetrakis(hydroxymethyl)imidazo[4,5-d]imidazole-2,5(1H,3H)-dione
- Cas Number:
- 5395-50-6
- Molecular formula:
- C8H14N4O6
- IUPAC Name:
- 1,3,4,6-tetrakis(hydroxymethyl)-octahydro-[1,3]diazolo[4,5-d]imidazole-2,5-dione
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- preparation with aqua dest., administered as solution
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Wiga
- Weight at study initiation: male animals 24 - 28 g, female animals 22 - 24 g
- Fasting period before study: 15 - 20 h before administration
- Diet (e.g. ad libitum): Herilan MRH-Haltung; Eggersmann KG
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- water
- Details on exposure:
- administered volume: 10 mL/kg body weight
- Doses:
- 2000 mg/kg, 700 mg/kg
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing:
mortality: 1 h, 1 d, 2 d, 7 d, 14 d
mean weight: beginning of test, 2 - 4 d, 7 d, 13 d
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, necropsy findings, pathology, mortality
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 2/10 dead animals
- Mortality:
- 2 dead male animals 14 d after administration of 2000 mg/kg. 20 % mortality in high dose level
- Clinical signs:
- staggering (2000 mg/kg)
spastic gait, piloerection, dyspnea, apathy, poor general state (700 and 2000 mg/kg) - Body weight:
- no effects observed
- Gross pathology:
- sacrificed animals: Cadaverous; no intraabdominal precipitates of the test substance or adhesions
Any other information on results incl. tables
Table 1: LD50 determination
Doses [mg/kg] |
No. of animals |
Dead animals after 14 days |
Mortality [%] |
Doses used for calculating LD50 |
700 |
10 |
0 |
0 |
|
2.000 |
10 |
2 |
20.0 |
* |
Table 2: Mean weight
Dose [mg/kg] |
2.000 |
700 |
|
Male animals |
Mean weight [g] |
|
|
Beginning of test |
28 |
24 |
|
After 2 – 4 d |
24,2 |
29,2 |
|
After 7 d |
24,8 |
30,8 |
|
After 13 d |
25,8 |
31,8 |
|
Female animals |
Beginning of test |
24 |
22 |
After 2 – 4 d |
23,8 |
25,8 |
|
After 7 d |
25,6 |
26,0 |
|
After 13 d |
27,6 |
26,2 |
Applicant's summary and conclusion
- Conclusions:
- After intraperitoneal injection, the LD50 for the test substance is > 2.000 mg/kg body weight.
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