Hexakis[μ-(acetato-O:O')]-μ3-oxo-triangulo-triruthenium acetate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

not specified

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Water samples were taken from the control and each replicate test vessel at 0 (fresh media), 24 and 96 (old media) hours.

Vehicle:

no

Details on test solutions:

Range-finding study conducted and based on this a limit test at 100 mg/L was conducted. For the definitive study, test material was dissolved directly in water with the aid of ultasonification. A 4000mg/L stock solution was prepared, which was then diluted to give a 100 mg/L test solution.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Fish were obtained from Brow Well Fisheries, UK. Fish acclimatised to test conditions from 1-13 November 2000. Feeding discontinued 24hrs pror to the start of the definitive study. Mean standard length of 4.2cm, mean weight of 0.96g. Loading rate 0.48 g bodyweight/litre.

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

14 ± 1°C

pH:

7.1-7.9

Dissolved oxygen:

9.3-10.2

Salinity:

Not applicalbe

Nominal and measured concentrations:

Exposed to a single concentration of 100 mg/L based on the results of the range finding test. Measured concentration remained near nominal, therefore LC50 values were based on nominal.

Details on test conditions:

Test vessel: 20 litre glass exposure vessels, covered to reduce evaporation
No of organisms per vessel: 10 fish per vessel
No of replicates per concentration: 2 replicates
Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortalities recorded

Validity criteria fulfilled:

yes

Conclusions:

The 96 hour LC50 of ruthenium acetate to Oncorhynchus mykiss was reported to be >100 mg/L.

Executive summary:

This study is GLP compliant and followed OECD guideline 203. A limit test at 100 mg/L was conducted. Test concentrations were analysed and were close to nominal, therefore the LC50 value is reported based on nominal concentration. It is suitable for use as a key study for this endpoint. The 96 hour LC50 of ruthenium acetate to Oncorhynchus mykiss was reported to be >100 mg/L.

Description of key information

The 96 hour LC50 of ruthenium acetate to Oncorhynchus mykiss was reported to be >100 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Wetton and Mullee (2001) is GLP compliant and followed OECD guideline 203. A limit test at 100 mg/L was conducted. Test concentrations were analysed and were close to nominal, therefore the LC50 value is reported based on nominal concentration. It is suitable for use as a key study for this endpoint. The 96 hour LC50 of ruthenium acetate to Oncorhynchus mykiss was reported to be >100 mg/L.

This is supported by Jianying (2004), a guideline study considered suitable for use as a supporting study for this endpoint. A single nominal concentration of 100 mg/L was used. Test solutions were renewed, but test concentrations were not analytically verified. The 96 hour LC50 of ruthenium acetate to Zebrafish was determined to be >100 mg/L.