Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1975
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report date:
1975

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Disodium [(9,10-dihydro-9,10-dioxo-1,4-anthrylene)bis(imino-4,1-phenyleneoxy)]bis(benzenesulphonate)
EC Number:
274-355-7
EC Name:
Disodium [(9,10-dihydro-9,10-dioxo-1,4-anthrylene)bis(imino-4,1-phenyleneoxy)]bis(benzenesulphonate)
Cas Number:
70161-19-2
Molecular formula:
C38H26N2O10S2.2Na
Test material form:
solid: particulate/powder
Details on test material:
Acid Green 40

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, industrial (adaptation not specified)
Duration of test (contact time):
31 d
Initial test substance concentrationopen allclose all
Initial conc.:
20 mg/L
Based on:
TOC
Initial conc.:
23.3 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
TOC removal
Details on study design:
TEST CONDITIONS
- Composition of medium: TOC content of medium: 108 mg/L

TEST SYSTEM
- Number of culture flasks/concentration: 1


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

Results and discussion

% Degradation
Key result
Parameter:
% degradation (TOC removal)
Value:
0
St. dev.:
0
Sampling time:
31 d

BOD5 / COD results

BOD5 / CODopen allclose all
Parameter:
BOD5
Value:
0 mg O2/g test mat.
Remarks on result:
other: Test item conc. 500 mg/L
Parameter:
COD
Value:
1 092 mg O2/g test mat.
Remarks on result:
other: Test item conc. 500 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The substance was found to be not inherently biodegradable (0% after 31 d based on TOC removal). The ratio BOD5/CSB does not indicate any potential for biodegradation.