Cobalt zinc silicate blue phenacite

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

adopted 1984-04-04

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Cobalt zinc silicate blue phenacite

EC Number:

270-208-6

EC Name:

Cobalt zinc silicate blue phenacite

Cas Number:

68412-74-8

Molecular formula:

Zn(2-x)Co(x)SiO4 0,7≤x≤1,1

IUPAC Name:

cobalt zinc silicate blue phenacite

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): "Farbkörper DUNKELBLAU 226579"
- Physical state: dark blue powder
- Storage condition of test material: room temperature

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

no data

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method:
The test substance is hardly soluble. Therefore, before starting the test, the test substance (100, 1000, and 10000 mg test item) was suspended in dilution water for daphnids and placed in a mechnical shaker (approximately 130 rpm) for 24 hours. After this time period of incubation the suspension was filtered (fluted filter; rinsed with deionised water before filtration was conducted). The clear filtrate (eluate) was used in the test without further dilution.
- Controls: blank control (assumed, not clearly stated in the study report)

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

EST ORGANISM
- Species: Daphnia magna, var. Straus
- Source: Dr. Knie LWA
- Age at study initiation: 6 hours ≥ age ≤24 hours
- Housing: housing in drinking water at room temperature (20 ± 1°C): photoperiod: 16 hours diffused lighting
- Food type: daily, Scenedesmus subspicatus

Study design

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

48 d

Test conditions

Hardness:

no data

Test temperature:

no data

pH:

Control:
- test start: 7.28
- test end: 7.37
Test vessel (10000 mg/L):
- test start: 7.27
- test end: 7.41
Test vessel (1000 mg/L)
- test start: 7.01
- test end: 7.40
Test vessel (100 mg/L)
- test start: 6.72
- test end: 7.39

Dissolved oxygen:

Control:
- test start: 8.5 mg O2/L
- test end: 8.3 mg O2/L
Test vessel (10000 mg/L):
- test start: 8.4 mg O2/L
- test end: 8.2 mg O2/L
Test vessel (1000 mg/L)
- test start: n.d.
- test end: n.d.
Test vessel (100 mg/L)
- test start: n.d.
- test end: n.d.

Salinity:

not applicable

Nominal and measured concentrations:

Nominal concentration: 100, 1000, and 10000 mg/L

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel: 5 daphnids
- one vessel for concentration / control

TEST MEDIUM / WATER PARAMETERS: dilution water

OTHER TEST CONDITIONS
- pH-value, water temperature and the amount of dissolved oxygen were measured at the begin and at the end of the test

EFFECT PARAMETERS MEASURED:
- percentage of immobile daphids were determined at 24 and 48 hours

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: aqueous extract

Details on results:

- Behavioural abnormalities: no immobilisation of daphnids was observed during the study.

Results with reference substance (positive control):

no data

Reported statistics and error estimates:

no data

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

EC50 (48 hours) > 10000 mg/L (aqueous extract)
No immobilisation of daphnids was observed during the study.