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EC number: 236-599-2 | CAS number: 13446-49-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Test followed international guidelines for the used test species, physicochemistry was measured and reported, proper statistics were applied
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- no
- Remarks:
- The study was carried out in compliance with the principles of good laboratory practice.
Test material
- Reference substance name:
- Sodium molybdate dihydrate
- IUPAC Name:
- Sodium molybdate dihydrate
- Reference substance name:
- Reference substance 001
- EC Number:
- 600-158-6
- Cas Number:
- 10102-40-6
- Molecular formula:
- H2 O. 1/2 Mo O4. Na
- Reference substance name:
- not availabble for the tested dihydrate
- IUPAC Name:
- not availabble for the tested dihydrate
- Details on test material:
- - Name of test material (as cited in study report): Sodium Molybdate Dihydrate
- Molecular formula (if other than submission substance): Na2MoO4⋅2H2O
- Molecular weight (if other than submission substance): 241.95 g/mol
- Physical state: solid (white crystalline powder)
- Analytical purity: 99.9% (dihydrate form) based on an analysed Molybdenum content of 39.6%
- Expiration date of the lot/batch: 31 December 2008
- Storage condition of test material: at room temperature (10-30°C) in the dark, dry
- no further significant details stated
Constituent 1
Constituent 2
Constituent 3
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples for total Mo (test initiation) and dissolved Mo (test initiation and termination, filtered through 0.45 µm) were taken from all treatments
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Pseudokirchneriella subcapitata
- Tested life stage: exponential growing algal cells
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 25 mg/ as CaCO3 (theoretical hardness, based on reported Ca- and Mg content of OECD medium)
- Test temperature:
- 24 degrees Celsius
- pH:
- pH of Test 1 at start of exposure:: 7.79 - 8.28
pH of Test 1 after 72 hours: 7.54-8.24
pH of test 2 at start of exposure: 7.15-7.59
pH of Test 2 after 72 hours: 7.54-8.35
pH of Test 3 at start of exposure:: 7.09 - 7.61
pH of Test 3 after 72 hours: 7.48-7.94
pH of test 4 at start of exposure: 7.71-8.26
pH of Test 4 after 72 hours: 7.58-8.03 - Nominal and measured concentrations:
- nominal: control, 46, 100, 220, 460, 1000, 2200, 4600 mg Mo/L
measured - dissolved (at start of Test 1):
measured - dissolved (at end of Test 1):- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable):closed
- Material, size, headspace, fill volume: glass 100mL Erlenmeyers
- Type of flow-through (e.g. peristaltic or proportional diluter): static
- Initial cells density: 10,000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes:
Test 1 & Test 4: OECD-medium prepared by UGent
Test 2 & Test 3: OECD-medium prepared by CIMM
OTHER TEST CONDITIONS
- Photoperiod: 24l/0D (according to OECD guidance)
- Light intensity: 85-125 μE m-2s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: ; electronic particle counter (coulter counter)
- end parameter: growth rate - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 234 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- Mo-dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: Test 1
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 366.2 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- Mo-dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: 294.4-455.5 (Test 1)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 568.9 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- Mo-dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: 1431.2-1719.8 (Test 1)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 236 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- Mo-dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: Test 2
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 318.6 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- Mo-dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: 278.9-363.9 (Test 2)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 094.5 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- Mo-dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: 1019.6-1174.8 (Test 2)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 49.6 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- Mo-dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: Test 3
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 61.2 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- Mo-dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: 36.5-102.7 (Test 3)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 390.9 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- Mo-dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: 312.4-489.2 (Test 3)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 48.6 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- Mo-dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: Test 4
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 72.6 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- Mo-dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: 48.3-109.2 (Test 4)
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 295 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- element
- Remarks:
- Mo-dissolved
- Basis for effect:
- growth rate
- Remarks on result:
- other: 247.8-351.3 (Test 4)
- Details on results:
- All validity criteria were fulfilled over the 72 hour exposure period.
- Reported statistics and error estimates:
- Growth rates based on daily cell density of the treatments during 72 hours were calculated according to OECD Guideline No. 201 (OECD, 2006). The effect concentrations (72h-ErC10 and 72h-ErC50) were estimated based on dissolved concentrations at the start of the test using a standard log-logistic concentration-response model. Calculations were performed using Statistica® software.
For the determination of the NOEC the methods recommended by OECD (2005) were used. The Jonckheere-Terpstra trend test (Lehmann, 1975) was used. All hypothesis testing was conducted one-sided at the α level of 0.05 as recommended by OECD (2005). Hypothesis testing was performed with SPSS16® software.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Following an internationally recognised standard test procedure for the evaluation of chronic toxicity of sodium molybdate for the freshwater green alga Pseudokirchneriella subcapitata, reliable (K1) EC10, EC50 and NOEC-values were derived. Effect levels for standard exposure period and end parameter were based on measured concentration levels.
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