p-anisylamine

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

for Read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of fish for the test chemical :
1.To evaluate toxicity of test material on Lipomyces starkeyi IFO10381
2.Aim of this study was to assess the effect of test chemical on the growth of microorganisms.

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: 4-Methoxybenzylamine
- IUPAC name: p-anisylamine
- Molecular formula: C8H11NO
- Molecular weight: 137.181 g/mole
- Smiles :O(c1ccc(cc1)CN)C
- Inchl: 1S/C8H11NO/c1-10-8-4-2-7(6-9)3-5-8/h2-5H,6,9H2,1H3
- Substance type: Organic
- Physical state: Liquid (Colorless to pale yellow)

Analytical monitoring:

not specified

Details on test solutions:

1.The compound was used as a methanol solution to produce final concentrations of 20 or 200 mg/L compound and 1% (v/v) methanol.

Test organisms (species):

other: 1.Lipomyces starkeyi IFO10381 , 2.Tetrahymena pyriformis

Details on inoculum:

1.Oleaginous yeast, Lipomyces starkeyi IFO10381 (IFO, Osaka, Japan) was maintained on YM agar at 25 °C. One loopful of yeast well grown on a YM agar slant was inoculated into 5 mL of YM broth and cultivated with 120 rpm rotary shaking at 27 °C for 2 days. The precultured broth was inoculated with 1% (v/v) inoculum into the MLA broth

2.- Laboratory culture: Exponential growth phase (log) culture were used

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

7 d

Test temperature:

1 and 2 . 27 °C

pH:

2)7.35

Nominal and measured concentrations:

1)20 and 200 mg/L

Details on test conditions:

1) The compound as a methanol solution was added to 5 mL of broth in a test tube to produce final concentrations of 20 or 200 mg/L compound and 1% (v/v) methanol at the start of the culture. As a control, 1% methanol was added to the broth. Yeasts were cultivated using a rotary shaker (120 rpm) at 27 °C for 7 days

2) Details on test conditions
TEST SYSTEM
- No. of vessels per concentration (replicates): ≥ 3

Reference substance (positive control):

not specified

Duration:

7 d

Dose descriptor:

other: EC14

Effect conc.:

200 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Read across 1

Duration:

48 h

Dose descriptor:

IC50

Effect conc.:

157 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Read across 2

Validity criteria fulfilled:

not specified

Executive summary:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity to microorganism of the test chemical 4-Methoxybenzylamine (2393 -23 -9).The studies are as mentioned below:

1.Antifungal activity of test material was tested at the dose concentrations of 20 and 200 mg/L on the fungi Lipomyces starkeyi IFO10381 for a duration of 7 days. The substance inhibited fungal growth by 14% at both concentrations.

2.Aim of this study was to assess the effect of test chemical on the growth of microorganisms Tetrahymena pyriformis (Ciliate). Test conducted under the static system for total exposure period of 48 hrs. ≥ 3 replicates were used. Based on the population growth rate inhibition of test organism Tetrahymena pyriformis (Ciliate) due to the test chemical , the IC50 was determine to be 157 mg/l. Thus based on the IC50, test chemical was consider as nontoxic.

Thus, based on the above summarised studies, 4-Methoxybenzylamine and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range 157- 200 mg/l

Description of key information

Toxicity to microorganisms:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity to microorganism of the test chemical 4-Methoxybenzylamine (2393 -23 -9).The studies are as mentioned below:

1.Antifungal activity of test material was tested at the dose concentrations of 20 and 200 mg/L on the fungi Lipomyces starkeyi IFO10381 for a duration of 7 days. The substance inhibited fungal growth by 14% at both concentrations.

2.Aim of this study was to assess the effect of test chemical on the growth of microorganisms Tetrahymena pyriformis (Ciliate). Test conducted under the static system for total exposure period of 48 hrs. ≥ 3 replicates were used. Based on the population growth rate inhibition of test organism Tetrahymena pyriformis (Ciliate) due to the test chemical , the IC50 was determine to be 157 mg/l. Thus based on the IC50, test chemical was consider as nontoxic.

Thus, based on the above summarised studies, 4-Methoxybenzylamine and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range 157- 200 mg/l

Key value for chemical safety assessment

EC50 for microorganisms:

200 mg/L

Additional information

Toxicity to microorganisms:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity to microorganism of the test chemical 4-Methoxybenzylamine (2393 -23 -9).The studies are as mentioned below:

1.Antifungal activity of test material was tested at the dose concentrations of 20 and 200 mg/L on the fungi Lipomyces starkeyi IFO10381 for a duration of 7 days. The substance inhibited fungal growth by 14% at both concentrations.

2.Aim of this study was to assess the effect of test chemical on the growth of microorganisms Tetrahymena pyriformis (Ciliate). Test conducted under the static system for total exposure period of 48 hrs. ≥ 3 replicates were used. Based on the population growth rate inhibition of test organism Tetrahymena pyriformis (Ciliate) due to the test chemical , the IC50 was determine to be 157 mg/l. Thus based on the IC50, test chemical was consider as nontoxic.

Thus, based on the above summarised studies, 4-Methoxybenzylamine and it’s structurally and functionally similar read across substance, it can be concluded that effect concetration value is in the range 157- 200 mg/l