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EC number: 278-127-8 | CAS number: 75214-58-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From July 20 to August 04, 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Acid Blue 193
- IUPAC Name:
- Acid Blue 193
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Harlan-Winkelmann GmbH, Borchen.
- Females nulliparous and non-pregnant: yes.
- Age at study initiation: 8 weeks for male, 9 - 10 weeks for female.
- Weight at study initiation: average 199 g for male and average 173 g for female.
- Housing: conventional Makrolon cages, type III
- Fasting period before study: ca 16 hours before and 4 hours after dosing.
- Diet: standard diet "fixed-formula" Altromin 1324 Pellets, ad libitum.
- Water: libitum.
- Acclimation period: 5 days adaptation.
ENVIRONMENTAL CONDITIONS
- Temperature: 22 ± 2 °C
- Humidity: 55 ± 5 %
- Photoperiod: 12 hrs dark / 12 hrs light.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- - Volume of administration: 10 mg/kg bw
- Preparation: test substance was formulated in the application medium immediately before the treatment.
- Administration: first animals of a gender (male) were treated with the intended dose. As soon as it could be seen that the dose did not result in a high lethality, the female animals were treated to determine any gender differences. - Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 5 animals per sex
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: on the day of application (day 1), animals were examined several times daily; once on weekends and public holidays in the following 14-day observation period, recording the type, beginning, duration and intensity of the clinical symptoms as well as possible dead animals away.
- Frequency of weighing: immediately before application (day 1), after one week and at the end of the 14-day observation period the animals were individually weighed. The individual application volume was calculated by means of the body weight obtained immediately before the application.
- Necropsy of survivors performed: yes.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortality was observed.
- Clinical signs:
- No poisoning phenomena were observed during the 14-day observation time. Eight hours up to a day after the administration of test substance, blue discolouration of faeces was seen.
- Body weight:
- The growth was not influenced in male and female rats.
- Gross pathology:
- No abnormalities were observed.
Applicant's summary and conclusion
- Interpretation of results:
- other: not classified, according to the CLP Regulation (EC) No 1272/2008
- Conclusions:
- LD50 (male and female) > 2000 mg/kg bw
- Executive summary:
Oral acute toxicity potential of test material was tested in rats, following procedures outlined in OECD guideline 401. A single dose of 2000 mg/kg bw was administered by gavage. Animales were observed for 14 days after dosing.
No death occurred during the main test and no poisoning phenomena were observed during the 14-day observation time. Eight hours up to a day after administration of test substance, blue discolouration of faeces was reported. No abnormalities were revealed by necropsy.
Conclusion
LD50 (male and female) > 2000 mg/kg bw
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