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EC number: 201-891-0 | CAS number: 89-25-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- January 2nd 1996
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: No information on the purity of the substance provided.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- Dated February 24th 1987
- Deviations:
- yes
- Remarks:
- Purity of test substance unknows. No analysis certificate available
- GLP compliance:
- no
Test material
- Reference substance name:
- 3-methyl-1-phenyl-5-pyrazolone
- EC Number:
- 201-891-0
- EC Name:
- 3-methyl-1-phenyl-5-pyrazolone
- Cas Number:
- 89-25-8
- Molecular formula:
- C10H10N2O
- IUPAC Name:
- 5-methyl-2-phenyl-2,4-dihydro-3H-pyrazol-3-one
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 503 0025
- Expiration date of the lot/batch: not specified
- Purity test date: no data
- Asect : white powder
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: not specified
- Stability under test conditions: not specified
- Solubility and stability of the test substance in the solvent/vehicle: not specified
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: not specified
- Final preparation of a solid: no preparation
FORM AS APPLIED IN THE TEST (if different from that of starting material)
OTHER SPECIFICS:
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Elevage Cunicole de Val de Selle, France
- Age at study initiation: not specified
- Weight at study initiation: 2.2 to 3.5 kg
- Housing:Individually in polystyrene or stainless steel cage
- Diet (e.g. ad libitum): Pellet diet 112C (U.A.R., France).Analysis was made (composition and contaminants) for each batch by the supplier. Moreover, this analysis were periodically made by CIT.
- Water (e.g. ad libitum): Drinking water filtered by 0.22 micron membran (F.G. Millipore) was delivered by bottle. Bacteriological, chemicals analysis and detection of the major contaminants (pesticides, heavy metals and nitrosamines) were periodically made.
- Acclimation period: at least 5 days before instillation
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18±3C
- Humidity (%): 30% to 70%
- Air changes (per hr): not specified
- Photoperiod (hrs dark / hrs light): 12h of light / 12h of dark
IN-LIFE DATES: From: To: Not specified
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: The second eye that did not received any product served as control.
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 100 mg
- Duration of treatment / exposure:
- 24 hours
- Observation period (in vivo):
- one hour, 24h, 48h and 72 h after instillations
- Number of animals or in vitro replicates:
- 3
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): The eyes were not rinsed after instillation of the test substances
- Time after start of exposure: 24h after instillation
SCORING SYSTEM:
based : -on Cornea ulceration and opacity (score 4 for opaque cornea to 0 for no ulceration and opacity) and opacity area (4 for three quarters to 1 for one quarter)
- Iris hemorrhage, reaction to light, swelling (score 2 for hemorrhage, gross destruction or no reaction to light, to 0 normal iris)
- Conjunctivae redness (score 3 for diffuse beefy red to 0 for normal), chemosis lids and/or nictating membranes swelling (score 4 for swellingh with lids more than half closed, to 0 normal)
-Discharge (score 2 for Discharge with moistening of lids and hairs adjacent to
lids, to 0 for absence of discharge)
TOOL USED TO ASSESS SCORE: fluorescein
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- conjunctivae score
- Remarks:
- Discharge
- Basis:
- animal #1
- Time point:
- other: 1 hour
- Score:
- ca. 2
- Max. score:
- 2
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- other: 1 hour
- Score:
- ca. 1
- Max. score:
- 1
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- other: Redness score
- Basis:
- animal #3
- Time point:
- other: 1 hour
- Score:
- ca. 1
- Max. score:
- 1
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- iris score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- iris score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0.3
- Max. score:
- 1
- Reversibility:
- fully reversible
- Irritation parameter:
- iris score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritation parameter:
- cornea opacity score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 0
- Reversibility:
- fully reversible
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- No ocular reactions were observed in all animals.
Any other information on results incl. tables
Rabbit identification |
EyesArea |
Ocularreactionsdescription |
Scores |
Irritation score |
Interpretation(+) (-) |
|||
1h |
24h |
48h |
72h |
|||||
01 |
Conjunctiva |
Chemosis |
0 |
0 |
0 |
0 |
0.0 |
(-) |
Redness |
0 |
0 |
0 |
0 |
0.0 |
(-) |
||
Discharge |
2 |
0 |
0 |
0 |
0.0 |
|
||
Iris |
0 |
0 |
0 |
0 |
0.0 |
(-) |
||
Corneaopacity |
Degree |
0 |
0 |
0 |
0 |
0.0 |
(-) |
|
Area |
0 |
0 |
0 |
0 |
0.0 |
|
||
Other |
|
* |
* |
* |
* |
|
||
Fluorescein |
/ |
U |
/ |
/ |
||||
02
|
Conjunctiva |
Chemosis |
0 |
0 |
0 |
0 |
0.0 |
(-) |
|
Redness |
0 |
0 |
0 |
0 |
0.0 |
(-) |
|
|
Discharge |
0 |
0 |
0 |
0 |
0.0 |
|
|
Iris |
|
0 |
0 |
0 |
0 |
0.0 |
(-) |
|
Corneaopacity |
Degree |
0 |
0 |
0 |
0 |
0.0 |
(-) |
|
|
Area |
0 |
0 |
0 |
0 |
0.0 |
|
|
Other |
|
* |
* |
* |
* |
|
||
Fluorescein |
|
/ |
U |
/ |
/ |
|||
03
|
Conjunctiva |
Chemosis |
1 |
1 |
0 |
0 |
0.3 |
(-) |
|
Redness |
1 |
1 |
0 |
0 |
0.3 |
(-) |
|
|
Discharge |
0 |
0 |
0 |
0 |
0.0 |
|
|
Iris |
|
0 |
0 |
0 |
0 |
0.0 |
(-) |
|
Corneaopacity |
Degree |
0 |
0 |
0 |
0 |
0.0 |
(-) |
|
|
Area |
0 |
0 |
0 |
0 |
0.0 |
|
|
Other |
|
* |
* |
* |
* |
|
||
Fluorescein |
|
/ |
U |
/ |
/ |
h = hours
(+) = Irritating according to E.E.C. criterias
(-) = not irritating according to E.E.C. criterias
* = None
U = Fluorescein used batch No. 4746
/ = Fluorescein not used
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- 100 mg of pure 1-Phenyl-3-methyl-5-Pyrazolone was instilled in rabbits eyes.It did not lead to irritation or corrosion. The registered substance is classified as non-irritant according to CLP regulation.
- Executive summary:
This study was performed to assess the Acute eye irritation/corrosion properties of the registered substance (1-Phenyl-3-methyl-5-pyrazolone). The study protocol is equivalent or similar to 405 OECD Guideline method (dated February, 24th 1987)
Material and Method
In this study, 3 New Zeland white rabbits were used. Acclimatation period was at least 5 days before instillation with registered substance. 100mg of pure registered substance was instilled into the conjunctival sac of the left eye of the animals after gently pulling the lower lid away from the eye ball. The upper and lower lids were held together for several seconds to avoid any loss of test substance, and the eyes were not rinsed after administration of the test substance. The untreated right eye served as control, and the ocular reactions at 1, 24, 48 and 72 hours after instillation were assessed.
Results
No ocular reactions were observed in all animals.
Conclusion
100 mg of pure 1-Phenyl-3-methyl-5-Pyrazolone instilled in rabbits eyes did not lead to ocular irritation or corrosion on all animals. The registered substance is classified as non-irritant according to CLP regulation.
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