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EC number: 204-100-7 | CAS number: 115-69-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 15 Jan 1997 - 05 Feb 1997
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- other: EN ISO 10712: 1995
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution in deionised water (12500 mg/L). pH adjusted to 7.2
- after addition of test substance replicates were shaken at 120 rpm and 23 ± 1 °C in the dark
- sterile preparation of replicates - Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: strain "Migula, DSM 50026"
- Preparation of inoculum for exposure: concentration of inoculum at test start 50 FNU (Formazin Nephelometric Units), single replicates at test start 5 FNU (1/10 dilution) - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 16 h
- Test temperature:
- 23 °C
- pH:
- 7.2
- Nominal and measured concentrations:
- nominal: 0, 31.5, 100, 312.5, 1000, 3125, and 10000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 250 mL glass Erlenmeyer flaks; all sterilised before use
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Culture / test medium: the following substances were diluted in water and filled up to 500 mL
solution 1:
NaNO3: 10 g
K2HPO4: 2.4 g
KH2PO4: 1.2 g
yeast extract: 1 g
solution 2:
NaNO3: 10 g
K2HPO4: 2.4 g
KH2PO4: 1.2 g
solution 3:
D(+)-Glucosemonohydrate x H2O: 10 g
solution 4:
MgSO4 x 7 H2O: 4 g
granular iron(III)citrate: 0.01 g
Stock solution: 18 g Agar, 50 mL solution 1, 125 mL solution 3, 100 mL solution 4, 1000 mL water; fluid medium diveded into 6-10 mL portions in culture vials, sealed, and sterilised for 10 min at 121 °C. Afterwards stored at 2 to 4 °C
OTHER TEST CONDITIONS
- Adjustment of pH: yes, 7.2
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : optical density of bacteria suspension was measured at 525 nm with a Merck SQ 118 photometer; measurements at the start and at the end of the test - Reference substance (positive control):
- yes
- Remarks:
- 2,3-Dichlorphenol
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: turbidity
- Key result
- Duration:
- 16 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: turbidity
- Results with reference substance (positive control):
- EC50 (16 h) > 10 <30 mg/L (demand value: 10 - 30 mg/L)
- Validity criteria fulfilled:
- yes
Reference
Table 1: turbidity measurements and median values (FNUs), standard deviations, variation coefficients, and growth inhibition
|
|
Measured FNUs |
||||||
|
Concentration (mg/L) |
Flask 1 |
Flask 2 |
Flask 3 |
Median value |
Standard deviation |
Variation coefficient |
Growth inhibition |
Control, 0 h |
|
6 |
|
|
6 |
|
|
|
Test substance, 16 h |
10000 |
568 |
570 |
565 |
568 |
2.5 |
0.4 |
6.9 |
|
3125 |
610 |
630 |
585 |
608 |
22.5 |
3.7 |
0.1 |
|
1000 |
600 |
610 |
603 |
604 |
5.2 |
0.9 |
0.8 |
|
312.5 |
610 |
600 |
608 |
606 |
5.2 |
0.9 |
0.6 |
|
100 |
618 |
613 |
630 |
620 |
9.0 |
1.5 |
-1.8 |
|
31.5 |
623 |
628 |
605 |
618 |
11.8 |
1.9 |
-1.5 |
Reference substance, 16 h |
30 |
5 |
5 |
5 |
5 |
0 |
0 |
100.2 |
|
10 |
610 |
605 |
618 |
611 |
6.292 |
1.03 |
-0.3 |
Control, 16 h |
|
603 |
615 |
610 |
609 |
6.292 |
1.033 |
0 |
Description of key information
Key value for chemical safety assessment
- EC50 for microorganisms:
- 10 000 mg/L
- EC10 or NOEC for microorganisms:
- 10 000 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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