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EC number: 696-543-1 | CAS number: 1369828-32-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 21 July 1997
- Deviations:
- yes
- Remarks:
- 2-aminoanthracene was used as sole positive control for metabolic activation
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-bromo-4-chloro-1-(propan-2-yl)benzene
- EC Number:
- 696-543-1
- Cas Number:
- 1369828-32-9
- Molecular formula:
- C9H10BrCl
- IUPAC Name:
- 2-bromo-4-chloro-1-(propan-2-yl)benzene
Constituent 1
Method
- Target gene:
- his operon, trp operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor-supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of male rats, treated with phenobarbital and β-naphthoflavone (80 mg/kg bw)
- Test concentrations with justification for top dose:
- Initial Mutation Test (plate incorporation test, Exp. I):
- 1.581, 5, 15.81, 50, 158.1, 500, 1581 and 5000 μg/plate (with and without metabolic activation, all Salmonella typhimurium strains and E. coli WPA uvr A)
Confirmatory Mutation Test (pre-incubation procedure, Exp. IIa):
- 1.581, 5, 15.81, 50, 158.1, 500, 1581 and 5000 μg/plate (with and without metabolic activation, only E. coli WPA uvr A)
Complementary Confirmatory Mutation Test (pre-incubation procedure, Exp. IIb):
- 0.01581, 0.05, 0.1581, 0.5, 1.581, 5, 15.81, 50, 158.1, 500, 1581 and 5000 μg/plate (without metabolic activation, all Salmonella typhimurium strains)
- 0.1581, 0.5, 1.581, 5, 15.81, 50, 158.1, 500, 1581 and 5000 μg/plate (with metabolic activation, all Salmonella typhimurium strains) - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- yes
- Remarks:
- untreated control
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO and distilled water
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- methylmethanesulfonate
- other: 4-nitro-1,2-phenylene-diamine (NPD); 2-aminoanthracene (2AA)
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- in Exp. I at 5000 μg/plate, -S9 mix, in the Exp. IIb at 500 and 158.1 μg/plate, +S9 mix, at 50 and 15.81 μg/plate, -S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- in the Exp. IIb 500 and 158.1 μg/plate, +S9 mix, at 50 and 15.81 μg/plate, -S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- in Exp. I at 500, 1581 and 5000 μg/plate, +/-S9 mix and in Exp. IIb at 500 and 158.1 μg/plate, +S9 mix, at 50, 15.81 and 5 μg/plate, -S9 mix.
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- in Exp. I at 5000 μg/plate, +/-S9 mix and at 1581 μg/plate, -S9 mix and in Exp. IIb at 500 and 158.1 μg/plate, +S9 mix, at 50 and 15.81 μg/plate, -S9 mix
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other:
- Remarks:
- Insolubility (microdrops) was detected in Exp. I at 5000 μg/plate +/-S9 mix and at 1581 μg/plate +S9 mix.
Applicant's summary and conclusion
- Conclusions:
- The potential of the test substance to induce gene mutations was examined in 4 Salmonella typhimurium strains TA98, TA100, TA102, TA1535 and TA1537 and in one E. coli strain WP2 uvrA in at two independent experiments, each carried out without and with metabolic activation. The first experiment was carried out as a plate incorporation test and the second as a preincubation test. Based on the results of the conducted study the test material did not exhibit mutagenic properties in bacteria with or without metabolic activation.
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