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EC number: 222-226-0 | CAS number: 3391-86-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- The study was conducted between 14 August 2017 and 17 August 2017 (biological phase).
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Deviations:
- yes
- Remarks:
- Deviating from the study plan the pH in the test medium exceeded the required upper limit (pH = 9) by 0.6 pH units. This deviation has no influence on the integrity of the study results, since all cell growth related validity criteria were fulfilled.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Oct-1-ene-3-ol
- EC Number:
- 222-226-0
- EC Name:
- Oct-1-ene-3-ol
- Cas Number:
- 3391-86-4
- Molecular formula:
- C8H16O
- IUPAC Name:
- oct-1-en-3-ol
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Batch No.: VE00440179
Colour and Appearance: colourless to pale yellow liquid
Purity: 99.6%
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples from the test solutions were analysed to determine actual levels of the test item at start of exposure period (time 0 hours) and following 72 hours of exposure. At time 0 hours the measured concentrations range between 64–91% and decrease after 72 hours exposure at between 0 (
Since all concentration levels were measured, the biological results are additionally calculated and reported based on geometric mean measured concentrations at each concentration level. Details are reported in the analytical phase report and in section 18.3 of the study report.
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test system used in this study was the freshwater algae Pseudokirchneriella subcapitata (SAG 61.81).
The organisms were originally supplied by Sammlung von Algenkulturen, Albrecht-von-Haller-Institut, Universität Göttingen, Germany.
Study design
- Test type:
- static
- Water media type:
- other: OECD medium (modified) + additional NaHCO3
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 22.8 to 23.3 °C
- pH:
- 8.3
- Nominal and measured concentrations:
- Test Concentrations (nominal): 0.237, 0.750, 2.37, 7.50, 23.7 and 75.0 mg test item/L and a control.
Measured: 0.057, 0.174, 0.505, 2.73, 6.27 and 28.5 mg test item/L
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.51 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence limit 0.902 - 2.09 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 2.56 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence limit 1.79 - 3.27 mg/L
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.05 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence limit 5.83 - 8.71 mg/L
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.505 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.174 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- Biological endpoints based on both nominal and mean measured concentrations are presented in the study report and dsummarised below in the section "any other information on results incl tables". However, because the test item concentration based on nominal concentration was not stable within ±20% throughout the exposure period, the biological endpoints based on the geometric mean measured concentrations are considered the most appropriate and reported in the effect concentrations table above.
Effects based on yield were also reported (see attached full study report). However, the preferred observational endpoint in the algal inhibition study is growth rate because it is not dependent on the test design (ECHA guidance Chapter R.7b v 2.0, OECD 201 Guidelines). The guideline includes the additional response variable of yield, to satisfy current regulatory requirements in some countries. The EU CLP regulation (No 1272/2008 and its adaption 286/2011) also states that classification should be based on the ErC50. Thus only the effects based on growth rate are presented in the above "effects concentration" table. Furthermore, the preferred observational endpoint in long-term studies is the EC10 value because it is derived from the dose response curve. In contrast the NOEC strongly depends on the experiment design (e.g. the concentrations used in the test). Thus the 72-h EC50 and EC10 based on growth rate are used for classification purposes, which were determined in this study to be 7.05 mg/L and 1.51 mg/L respectively.
Any other information on results incl. tables
Analytical findings
Samples from the test solutions were analysed to determine actual levels of the test item at start of exposure period (time 0 hours) and following 72 hours of exposure. At time 0 hours the measured concentrations range between 64–91% and decrease after 72 hours exposure at between 0 (<LOD) and 18% of the nominal concentrations. Therefore, the test item concentration based on nominal concentration was not stable within ±20% throughout the exposure period.
Since all concentration levels were measured, the biological results are additionally calculated and reported based on geometric mean measured concentrations at each concentration level. Details are reported in the analytical phase report and in section 18.3.
Biological findings
A clear concentration-response relationship was observed for both biological parameters growth rate and yield during the exposure period. The resulting endpoints are presented in Table 10 and Table 11, respectively, based on geometric mean measured concentrations and based on nominal concentrations expressed in mg/L.
The following ECx, NOEC and LOEC values for the parameters cell number and biomass expressed in yield and growth rate, based on statistical evaluation of biological results and measured geometric mean concentrations of the test item (Amyl Vinyl Carbinol).
Summary of biological results based on measured test item concentrations in mg Amyl Vinyl Carbinol/L.
Endpoints [mg Amyl Vinyl Carbinol/L]
Parameter EC10 EC20 EC50 NOEC LOEC
Yield 0.546 0.991 2.44 0.505 0.174
(95% cI) (0.336–0.755) (0.708–1.25) (2.11–2.75)
Growth Rate 1.51 2.56 7.05 0.505 0.174
(95% cI) (0.902–2.09) (1.79–3.27) (5.83–8.71)
cl = confidence limit
NOEC, LOEC and ECx values for the parameters cell number and biomass expressed in yield and growth rate are based on statistical evaluation of biological results and nominal concentrations in mg test item/L were given in the following table.
Summary of biological results based on nominal concentrations in mg test item/L.
Endpoints [mg test item/L]
Parameter EC10 EC20 EC50 NOEC LOEC
Yield 1.66 3.02 7.47 0.750 2.37
(95% cI) (1.42–1.89) (2.71–3.31) (7.06–7.89)
Growth Rate 5.27 8.72 22.8 0.750 2.37
(95% cI) (4.14–6.38) (7.31–10.1) (20.6–25.3)
cl = confidence limit
Validity of the test:
All validity criteria were fulfilled as required by the study plan:
Required: |
Found: |
Mean biomass increase in the control cultures:factor of at least 16 within the 72-hour test period |
237 |
Mean coefficient of variation for section-by-section specific growth rates in the control cultures:up to 35% |
17.4% |
Coefficient of variation of average specific growth rates during test period in replicate control cultures:up to 7% |
1.4% |
The study is therefore considered to be valid.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72-h EC50 and EC10 based on growth rate are used for classification purposes, which were determined in this study to be 7.05 mg/L and 1.51 mg/L respectively based on geometric mean measured concentrations.
- Executive summary:
The impact of the test item Amyl Vinyl Carbinol on the growth of the freshwater green algal species Pseudokirchneriella subcapitata was investigated in a 72-hour static test according to the OECD Guideline for the Testing of Chemicals, No. 201 (2011). Based on measured concentrations, the 72h EC50 of Pseudokirchneriella subcapitata was found to be 7.05 mg/L. The corresponding 72 ErC10 is 1.51 mg/L.
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