Salbutamol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Salbutamol

EC Number:

242-424-0

EC Name:

Salbutamol

Cas Number:

18559-94-9

Molecular formula:

C13H21NO3

IUPAC Name:

4-[2-(tert-butylamino)-1-hydroxyethyl]-2-(hydroxymethyl)phenol

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

other: Medium M7

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg/l expressed as CaCO3

Test temperature:

21.0 C

pH:

pH 8.0 +/- 0.2 after aeration

Nominal and measured concentrations:

0.1, 1.0, 10 and 100 mg/l of Salbutamol Base.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present test, Salbutamol Base induced no effects in Daphnia magna exposed to a concentration of 100 mg/l. Hence, the 48h-EC50 for mobility of Daphnia magna was above 100 mg/l, the maximum concentration to be tested.

Executive summary:

A limit test was combined with a range-finding test exposing daphnids to Salbutamol Base concentrations of 0.1, 1.0, 10 and 100 mg/l. The 100 mg/l test group consisted of two vessels containing 10 daphnids per vessel. The 0.1,1.0 and 10 mg/l test groups consisted of one vessel containing 10 daphnids.

The study did not induce sampling for determination of actual exposure concentrations.

Salbutamol Base induced no effects in Daphnia magna exposed to a concentration of 100 mg/l. Hence, the 48h-EC50 for mobility of Daphnia magna was above 100 mg/l, the maximum concentration to be tested.