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Diss Factsheets
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EC number: 906-936-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vivo
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Read across based on category approach. Please see category document for further information.
Data source
Reference
- Reference Type:
- publication
- Title:
- Metabolism and disposition of diethylene glycol in rat and dog
- Author:
- Mathew, J.M., Parker, M.K., Matthews, H.B.
- Year:
- 1 991
- Bibliographic source:
- Drug Metabolism and Disposition 19(6): 1066-1070
Materials and methods
- Principles of method if other than guideline:
- Occuded dermal exposure to DEG was on the clipped backs of rats. Breath, urine, feces, and blood was collected and radioactivity measured. Urine was analyzed for DEG and its metabolites by HPLC.
- GLP compliance:
- no
Test material
- Reference substance name:
- 2,2'-oxydiethanol
- EC Number:
- 203-872-2
- EC Name:
- 2,2'-oxydiethanol
- Cas Number:
- 111-46-6
- Molecular formula:
- C4H10O3
- IUPAC Name:
- 2,2'-oxydiethanol
Constituent 1
- Specific details on test material used for the study:
- DEG was purchased from Aldrich Chemical Co, Inc. (Milwaukee, WI). [U-14C]-DEG, specific activity 14 mCi/mmol, was obstained from Amersham Laboratories (Buckinghamshire, UK) and was further purified by HPLC to a radiochemical purity of 98%.
- Radiolabelling:
- yes
- Remarks:
- [U-14C]-DEG
Test animals
- Species:
- rat
- Strain:
- Fischer 344
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- Adult male Fischer 344 rats (220-280 g) were purchased from Charles River Breeding Laboratories, Inc. (Raleigh, NC). All animals were quarantined at least 1 week before they were used. Rats were fed Certified Purina Rodent Chow #5002 and furnished tap water ad libitum. During experiments rats were housed individually in glass metabolism chambers which provide for separate collection of urine, feces, and breath.
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Doses:
- The dermal dose formulation contained ~25 microCi of radiolabel and 50 mg of unlabeled DEG per animal.
- No. of animals per group:
- 3-5
- Details on study design:
- Dermal doses were administered onto a 12-cm2 area of skin in the intrascapular area on the backs of rats. Hair had been clipped from the area the previous day, and each rat was inspected to make sure the skin had not been nicked. After dosing, a nonocclusive protective appliance was glued over the dose area.
Determination of Radioactivity. Radiolabeled components eliminated in breath were collected separately by drawing air through a cold trap (-60°C) containing 100 ml of ethanol, and then through two traps, each containing 400 ml of 1 N NaOH. Aliquots of urine and trapping solution from the breath traps were added directly to vials containing scintillation cocktail (Scintiverse E, fisher Chemical Co.). Samples of tissue, feces, and blood (0.2-0.3 g) were digested in Soluene-350 (2 ml; Packard Instrument Co.). After digestion, samples requiring bleaching were decolorized with perchloric acid/hydrogen peroxide prior to addition of scintillation cocktail (Scintiverse E). Samples containing base and scintillate (e.g., sodium hydroxide. sodium carbonate, or Soluene-350) were kept in the dark overnight before they were assayed by liquid scintillation spectrometry.
Analysis of Biological Samples by HPLC. Urine was analyzed by HPLC for unchanged DEG and its metabolites.
Metabolites were isolated and identified by HPLC and NMR.
Results and discussion
- Absorption in different matrices:
- Dermally administered DEG (50 mg/12cm2) was slowly and steadily absorbed. Approximately 3% of the applied dose was absorbed per day and excreted in the urine over a 72 hour period. A total of 9.1 +/- 1.5% of the dose was recovered in the excreta, and 0.9 +/- 0.3% was recovered in tissues.
Percutaneous absorption
- Time point:
- 72 h
- Dose:
- 50 mg / 12 cm2
- Parameter:
- percentage
- Remarks:
- recovered in excreta
- Absorption:
- 9.1 %
Applicant's summary and conclusion
- Conclusions:
- DEG slowly penetrated the skin of rats after application of 50 mg to a 12 cm2 area. Only about 10% of the dose was absorbed in 72 hr of exposure and the absorbed dose appeared to have the same fate as doses administered iv or orally.
- Executive summary:
DEG slowly penetrated the skin of rats after application of 50 mg to a 12 cm2 area. Only about 10% of the dose was absorbed in 72 hr of exposure and the absorbed dose appeared to have the same fate as doses administered iv or orally.
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