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EC number: 257-440-3 | CAS number: 51812-80-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Experimental start date 02 Mar 2017 - experimental completion date 03 Mar 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 017
- Report date:
- 2017
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- adopted 22 July 2010
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3-(D-gluconoylamino)propyl(2-hydroxyethyl)dimethylammonium chloride
- EC Number:
- 257-440-3
- EC Name:
- 3-(D-gluconoylamino)propyl(2-hydroxyethyl)dimethylammonium chloride
- Cas Number:
- 51812-80-7
- Molecular formula:
- C13H29N2O7.Cl
- IUPAC Name:
- 1-Propanaminium, 3-(D-gluconoylamino)-N-(2-hydroxyethyl)-N,N-dimethyl-, chloride
- Test material form:
- liquid: viscous
- Details on test material:
- CR Test item 207516/A
Identification Quaternium-22
Appearance Brown paste (determined by Charles River Den Bosch)
Batch 0001954895
Purity/Composition > 90%
Test item storage In refrigerator (2-8°C)
Stable under storage conditions until 16 June 2017 (retest date)
Constituent 1
- Specific details on test material used for the study:
- Test item 207516/A
Identification Quaternium-22
Appearance Brown paste (determined by Charles River Den Bosch)
Batch 0001954895
Purity/Composition > 90%
Test item storage In refrigerator (2-8°C)
Stable under storage conditions until 16 June 2017 (retest date)
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A stock solution of 10 g/L was prepared by adding 4.96565 g test item to 500 mL of Milli-RO water (tap water purified by reverse osmosis; Millipore Corp., Bedford, Mass., USA). Magnetic stirring for 34 minutes was applied to accelerate dissolution and to ensure homogeneity. Volumes of the clear brown/yellow stock solution (pH 5.7) corresponding to the test concentration were then added to the test media. Optimal contact between the test item and test organisms was ensured by applying continuous aeration and stirring.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Source: Municipal sewage treatment plant: 'Waterschap Aa en Maas', 's- Hertogenbosch, The Netherlands.
Preparation of the sludge: The sludge was coarsely sieved (1 mm) and allowed to settle. The supernatant was removed and ISO-medium was added. A small amount of the sludge was weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.0 g/L of sludge, as used for the test). The pH was 7.7 on the day of testing. The batch of sludge was used one day after collection; therefore 50 mL of synthetic medium (=sewage feed) was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use
Medium: Adjusted ISO-medium, formulated using RO-water (tap water purified by reverse osmosis; GEON Waterbehandeling, Berkel-Enschot, The Netherlands) with the following composition:
CaCl2.2H2O 211.5 mg/L
MgSO4.7H2O 88.8 mg/L
NaHCO3 46.7 mg/L
KCl 4.2 mg/L
Rationale Recognized by international guidelines as the recommended test system.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- ranged between 18 and 21°C
- pH:
- between 7.2 and 8.3
- Dissolved oxygen:
- above 60-70% saturation (60% of air saturation is > 5 mg/L at 20°C)
- Nominal and measured concentrations:
- Combined limit/range-finding: 10, 100 and 1000 mg/L
- Details on test conditions:
- Vessels All glass open bottles/vessels.
Milli-RO / Milli-RO water Tap water purified by reverse osmosis (Millipore Corp., Bedford, Mass., USA).
Synthetic medium 16 g peptone
(=sewage feed) 11 g meat extract
3 g urea
0.7 g NaCl
0.4 g CaCl2.2H2O
0.2 g MgSO4.7H2O
2.8 g K2HPO4
Dissolved in Milli-RO water, made up to 1 litre and filtered. The pH was within 7.5 ± 0.5.
Air supply Clean, oil-free air.
Performance of the test The synthetic medium (= sewage feed, 16 mL) and an appropriate amount of the test item stock were mixed and made up to 250 mL with Milli-RO water. The pH was determined. Thereafter, 250 mL activated sludge was added. This was the start of the test. After the 3-hour contact time the oxygen consumption was recorded for a period of approximately 10 minutes. During measurement, the sample was not aerated but continuously stirred on a magnetic stirrer.
pH: The pH was determined in the remaining part of the reaction mixture. This procedure was repeated for all test/reference item concentrations and controls.
Temperature: the medium temperature was recorded continuously in two temperature control vessels. The temperature control vessels were identically prepared compared to the control vessels. A temperature control vessel with a REES sensor was placed in each fume cupboard of the climate room.
Oxygen recording: Determination of oxygen was performed with multiple oxygen sensors connected to a BlueBox (GO-Systemelektronik GmbH, Germany), a multichannel measuring and controlling system. - Reference substance (positive control):
- yes
- Remarks:
- 3,5- dichlorophenol: 1.0, 3.2, 10 and 32 mg/L.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this present test Quaternium-22 was not toxic to waste water bacteria (activated sludge) at 1000 mg/L.
The EC50 was above 1000 mg/L. - Executive summary:
The influence of Quaternium-22 on the respiration rate of activated sludge was investigated after a contact time of 3 hours.The study procedures described in this report were based onthe OECD guideline No. 209, 2010.
The batch of Quaternium-22 tested was a brown paste (UVCB) with a purity of > 90% (water content <10%).No correction was made for the purity/composition of the test item.
A stock solution of 10 g/L was prepared. Magnetic stirring for 34 minutes was applied to accelerate dissolution and to ensure homogeneity.Volumes of the clear brown/yellow stock solution corresponding to the test concentration were then added to the test media. Optimal contact between the test item and test organisms was ensured by applying continuous aeration and stirring. Thereafter, oxygen consumption was recorded for approximately 10 minutes.
In a combined limit/range-finding test concentrations of 10, 100 and 1000 mg/L were tested. The highest concentration was tested in triplicate; lower concentrations consisted of one replicate. Furthermore, at 1000 mg/L an abiotic control (1 replicate) was tested.Responses were compared to the blank.
No statistically significant inhibition of the respiration rate of the sludge was recordedat a concentration of 1000 mg Quaternium-22 per litre.Therefore, the EC50was above the highest concentration tested (1000 mg/L).
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