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EC number: 272-599-9 | CAS number: 68892-13-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- This study was in accordance with the EC Guideline and GLP. The test substance was equivalent to submission substance identity but the degree of purity was not available. Furthermore, no sufficient data was available to say that all validity criteria were fulfilled.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- The certificate copy was not included in the study report
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Inoculum comes from influent of Saint Germain au Mont d’Or STP; sampling date: 1998-02-04; no data was available about sampling method
- Laboratory culture: no data available
- Method of cultivation: no data available
- Storage conditions: no data available
- Storage length: 24 hours
- Preparation of inoculum for exposure: Sampling was filtered through glass fiber (elimination of big size particles) and the filtrate was centrifuged (no data was available about the centrifugation duration, speed and temperature). The centrifugation pellet was washed and recovered in a Ringer ¼ solution.
- Pretreatment: Inoculum was not pre-exposed to the test substance
- Concentration of sludge: no data available
- Initial cell/biomass concentration: 2.5*10E06 bacteria/mL (estimated by count in Petri dish). Inoculum was used at 1% in the test system
- Water filtered: yes
- Type and size of filter used, if any: The filter used was in glass fiber but no data was available about its size - Duration of test (contact time):
- 48 d
- Initial conc.:
- 20 other: mg/kg
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: see table below
- Additional substrate: see below
- Solubilising agent (type and concentration if used): No solubilising agent was used.
- Test temperature: 21.4 to 22.2°C
- pH: no data available
- pH adjusted: no data available
- CEC (meq/100 g): no data available
- Aeration of dilution water: no data available
- Suspended solids concentration: no data available
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Glassy Erlenmeyer flasks of 5 L
- Number of culture flasks/concentration: 3 culture flasks
- Method used to create aerobic conditions: no data available.
- Measuring equipment: no data available
- Test performed in closed vessels due to significant volatility of test substance: no data available
- Test performed in open system: no data available
- Details of trap for CO2 and volatile organics if used: Barita traps were used to trap CO2 (no detail was available)
SAMPLING
No data available
CONTROL AND BLANK SYSTEM
- Inoculum blank: 2 flasks (Inoculum in the mineral medium without the test substance)
- Abiotic sterile control: 1 flask (test substance in the mineral medium without the inoculum and with NaN3 10 g/L at 1% in the test solution)
- Adsorption control: no flask
- Toxicity control: 1 flask (test substance and reference substance with the inoculum in the mineral medium at 20 mg/kg for each one)
- other: Reference substance: 1 flask (reference substance with the inoculum in the mineral medium at 20 mg/kg)
STATISTICAL METHODS:
No data available - Reference substance:
- acetic acid, sodium salt
- Preliminary study:
- No preliminary study was performed
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 30
- Sampling time:
- 28 d
- Remarks on result:
- other: Result obtained by direct introduction of the substance
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 41
- Sampling time:
- 48 d
- Remarks on result:
- other: Result obtained by direct introduction of the substance
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 48
- Sampling time:
- 28 d
- Remarks on result:
- other: Results obtained by introduction of the substance after the store on sand.
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 54
- Sampling time:
- 48 d
- Remarks on result:
- other: Results obtained by introduction of the substance after the store on sand.
- Details on results:
- The use of solid carrier (sand) gave better results than the direct introduction of the substance into the test medium.
No degradation was measured before 28 days in the abiotic control. Then 53.5 mg of CO2 were measured between 28 days and the end of test corresponding to 23% of degradation.
The test was prolonged until 48 days because the plateau has not been reached by day 28 (see table in section "overall remarks"). The biodegradation percentage of the test substance was below the pass level for ready biodegradability (i.e. 60%) at 28 days and at the end of the test. - Results with reference substance:
- 67% of degradation after 14 days
91% of degradation after 48 days
The degradation percentage of the reference substance was higher than 60% after 14 days.
The result obtained with the reference substance was valid. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: Not readily biodegradable
- Conclusions:
- On the basis of these results, it can be concluded that Rhodiastab 92 is not readily biodegradable.
- Executive summary:
Rhodiastab 92 was tested for ready biodegradability according to the EU method C4-C (modified Sturm test), described in the Annex V of the Directive 67/548/EEC, and in compliance with the Good Laboratory Practice.
The biodegradation was calculated from the CO2 released measurements compared to the theoretical amount of CO2 for complete mineralization of the test substance. Due to its low water solubility, 2 test suspension flasks were prepared by direct addition of Rhodiastab X2, and a third test suspension flask was prepared using sand as solid carrier.
As a plateau was not reached in 28 days, the test was continued up to 48 days. The positive control was degraded to 67% within 14 days and no inhibition was detected with the toxicity control. All the validity criteria were fulfilled.
For the flasks prepared by direct addition of Rhodiastab X2, the percentages of biodegradation were 30% and 41%, within 28 days and 48 days respectively. The flask prepared with a solid carrier showed a higher level of biodegradation with values of 48% and 54%, within 28 days and 48 days respectively.
On the basis of these results, the Rhodiastab X2 is considered as not readily biodegradable.
Reference
Degradation percentages according to time:
Type of suspension |
Vessel n° |
% degradation at sampling time (days) |
|||||||||
0 |
1 |
4 |
7 |
14 |
21 |
28 |
35 |
42 |
48 |
||
Test sample |
FE1 |
0 |
0 |
0 |
7 |
16 |
22 |
29 |
33 |
35 |
39 |
FE2 |
0 |
0 |
0 |
6 |
17 |
25 |
31 |
39 |
40 |
43 |
|
FE3 |
0 |
0 |
2 |
15 |
31 |
42 |
48 |
51 |
52 |
54 |
|
Reference substance |
FC |
0 |
12 |
47 |
57 |
67 |
81 |
89 |
90 |
90 |
91 |
Inhibition control |
FI |
0 |
7 |
26 |
35 |
44 |
52 |
58 |
61 |
63 |
65 |
Abiotic sterile control |
FA |
0 |
0 |
1 |
1 |
0 |
0 |
0 |
3 |
12 |
23 |
Validity criteria:
- The inorganic carbon content of the test chemical suspension in the mineral medium at the beginning of the test must be less than 5% of the total carbon content:
No data available
- The total CO2 evolution in the inoculums blank at the end of the test should not normally exceed 40 mg/L medium:
YES, the CO2 quantities were respectively of 14 and 10.6 mg/L at 48 days.
- The difference of extremes of replicate values of the removal test chemical at the plateau, at the end of the test or at the end of the 10 day window, as appropriate, is less than 20%:
YES, the difference of replicates values at the end of the test is less than 20%
- The degradation percentage of the reference substance has reached the level for ready biodegradability for 14 days:
YES, the degradation percentage of the reference substance was 67% after 14 days.
Description of key information
A ready biodegradability test was performed according to the EU method C4-C (modified Sturm test), described in the Annex V of the Directive 67/548/EEC, and in compliance with the Good Laboratory Practice. The biodegradation was calculated from the CO2 released measurements compared to the theoretical amount of CO2 for complete mineralization of the test substance. Due to its low water solubility, 2 test suspension flasks were prepared by direct addition of Rhodiastab X2, and a third test suspension flask was prepared using sand as solid carrier.
As a plateau was not reached in 28 days, the test was continued up to 48 days. For the flasks prepared by direct addition of Rhodiastab X2, the percentages of biodegradation were 30% and 41%, within 28 days and 48 days respectively. The flask prepared with a solid carrier showed a higher level of biodegradation with values of 48% and 54%, within 28 days and 48 days respectively.
On the basis of these results, the Rhodiastab X2 is considered as not readily biodegradable.
The positive control was degraded to 67% within 14 days and no inhibition was detected with the toxicity control. All the validity criteria were fulfilled.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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