Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: other routes

Currently viewing:

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Study period:
09/1964
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1964

Materials and methods

Test guideline
Qualifier:
no guideline followed
Deviations:
not applicable
Principles of method if other than guideline:
Method: other: Farbenfabriken Bayer AG; details described in sections "Test animals" and "Administration/Exposure"
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Cyclododecane
EC Number:
206-033-9
EC Name:
Cyclododecane
Cas Number:
294-62-2
Molecular formula:
C12H24
IUPAC Name:
cyclododecane
Details on test material:
Test substance: "pure" cyclododecane, no further information

Test animals

Species:
rat
Strain:
not specified
Sex:
not specified
Details on test animals or test system and environmental conditions:
no details

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: acetone and vegetable oil 1:10
Details on exposure:
ADMINISTRATION: 
- Volume administered or concentration: 5 ml/kg bw
Doses:
100, 500, 1000, 1250, or 1500 mg/kg bw
No. of animals per sex per dose:
10 animals per dose
Control animals:
no
Details on study design:
no details
Statistics:
LD50 calculation: according to LICHTFIELD and WILCOXON

Results and discussion

Effect levels
Sex:
not specified
Dose descriptor:
LD50
Effect level:
1 225 mg/kg bw
95% CL:
1 074 - 1 398
Mortality:
MORTALITY: 
- Time of death: within 2-5 days
- Number of deaths at each dose:   100 or 500 mg/kg: 0   1000 mg/kg: 1/10   1250 mg/kg: 6/10   1500 mg/kg: 8/10
Clinical signs:
CLINICAL SIGNS: No clinical signs were observed in the 100 mg/kg bw  group, but all animals that received higher doses showed symptoms:  
breathing difficulties, spasticity, impairment of general condition,  palmospasms, and at the highest doses also narcosis.
Body weight:
no data
Gross pathology:
no data
Other findings:
no other findings

Any other information on results incl. tables

MORTALITY: 
- Number of deaths at each dose: 
   100 or 500 mg/kg: 0
  1000 mg/kg: 1/10
  1250 mg/kg: 6/10
  1500 mg/kg: 8/10
  LD50 = 1225 (1074-1398) mg/kg (Litchfield & Wilcoxson)

Applicant's summary and conclusion

Conclusions:
Under the conditions of this study the LD 50 for acute intraperitoneale toxicity of Cyclododecane is determined to be 1225 mg/kg of body weight in
rats.
Executive summary:

The test item Cyclododecane was applied intraperitoneale once to 5 dose-groups of 10 rats in doses of 100, 500, 1000, 1250 and 1500 mg/kg bw as solution in acetone/vegetable oil 1:10. The dose groups from 1000 mg/kg showed mortality. No clinical signs were observed in the 100 mg/kg bw  group, but all animals that received higher doses showed symptoms:  breathing difficulties, spasticity, impairment of general  condition,  palmospasms, and at the highest doses also narcosis.

Under the conditions of this study the LD 50 for acute intraperotoneale toxicity of Cyclododecane is determined to be 1225 mg/kg of body weight in rats.