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Diss Factsheets
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EC number: 209-002-8 | CAS number: 551-93-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Developmental toxicity / teratogenicity
Administrative data
- Endpoint:
- developmental toxicity
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Maternal Ingestion of Ortho-Aminoacetophenone During Gestation Affects Intake by Offspring
- Author:
- Nolte, D.L. and Mason, J.R.
- Year:
- 1 995
- Bibliographic source:
- Physiology & Behavior, Vol. 58, No. 5, pp. 925-928
Materials and methods
- Principles of method if other than guideline:
- - Principle of test: The effect of maternal ingestion of the test substance via drinking water during gestation on substance intake by offspring via drinking water
- Short description of test conditions: Pairs of male and female mice were given the test substance at 0.1% in drinking water or tap water (negative control) from mating until gestation day 18. All litters were reduced to 6 male pups on postparturition day 11, and 15 litters each from the test and control groups were selected for subsequent behavioural testing. Litters were weaned on postparturition day 21.
From each litter consisting of 6 males, 3 pups were selected for behavioural testing at weaning from postnatal day 25 (first cohort), the remaining 3 pups were subject to behavioural testing as subadults from postnatal day 87 (second cohort). Parental males were likewise tested after having been separated from the females on gestation day 18 (third cohort).
- Parameters analysed / observed: For behavioural testing, following an adaptation and pre-treatment period without test substance exposure, each cohort was divided into 3 subcohorts which were presented the test substance at 0.1, 0.25 and 0.5% in drinking water for 3 h, respectively. Water intake was measured and compared to the 3-hour water intake measured during the pre-treatment period in order to determine the relative avoidance both per test substance concentration and treatment during gestation. - GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- 2'-aminoacetophenone
- EC Number:
- 209-002-8
- EC Name:
- 2'-aminoacetophenone
- Cas Number:
- 551-93-9
- Molecular formula:
- C8H9NO
- IUPAC Name:
- 1-(2-aminophenyl)ethan-1-one
- Test material form:
- solid: crystalline
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- CF-1
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: 90-95 days
- Housing: animals were caged as pairs during mating and gestation up to gestation day 18, and individually thereafter.
- Diet: Wayne Rodent Blox, ad libitum
- Water: tap water, ad libitum (except during adaptation and pre-treatment period)
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 23
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: drinking water
- Vehicle:
- water
- Details on exposure:
- PREPARATION OF DOSING SOLUTIONS: the test substance was mixed with tap water to prepare a 0.1% (w/w) stock emulsion.
- Analytical verification of doses or concentrations:
- not specified
- Details on mating procedure:
- - Impregnation procedure: cohoused
- M/F ratio per cage: 1/1
- Length of cohabitation: from mating to gestation day 18
- Proof of pregnancy: vaginal plug referred to as day 0 of pregnancy - Duration of treatment / exposure:
- from mating to gestation day 18
- Frequency of treatment:
- continuous
- Duration of test:
- Parental animals were treated with the test substance until gestation day 18.
Offspring males were subject to behavioural testing from post-natal day 25 (first cohort), and post-natal day 87 (second cohort).
Parental males were subject to behavioural testing after gestation day 18 (third cohort).
Doses / concentrationsopen allclose all
- Dose / conc.:
- 0.1 other: %
- Remarks:
- during mating and gestation (parental animals)
- Dose / conc.:
- 0.1 other: %
- Remarks:
- for behavioural testing (offspring and parental males)
- Dose / conc.:
- 0.25 other: %
- Remarks:
- for behavioural testing (offspring and parental males)
- Dose / conc.:
- 0.5 other: %
- Remarks:
- for behavioural testing (offspring and parental males)
- No. of animals per sex per dose:
- Parental animals (control): 22 pairs
Parental animals (test group): 22 pairs
Offspring: 6 males per litter (out of 15 litters) - Control animals:
- yes, concurrent no treatment
- Details on study design:
- - Dose selection rationale: The test substance is known to be aversive to mice. The substance has been reported to reduce fluid intake by mice and to virtually eliminate ingestion at concentrations 1% and above.
Results and discussion
Any other information on results incl. tables
All subcohorts within an age cohort ingested similar amounts of water during the pretreatment period. At 26 days of age, pups born to mothers that ingested the test substance during gestation ingested more test substance-treated water than did offspring from mothers given water (F = 11.2070, df 1.84, P = 0.0016). Further, regardless of exposure, there was an inverse relationship between intake and test substance concentration (F = 259.249, df 2.84, P < 0.0001). There was not an interaction between exposure and test concentration (F = 0.703, df 2.84, P > 0.35).
Offspring tested at 88 days of age responded similarly to those tested at 26 days of age. Prior experience with the test substance enhanced their intake during the treatment period (F = 8.555, df 1.83, P = 0.0047), mice restricted their intake with increasing test concentrations (F = 113.701, df 2.83, P < 0.0001) and no interaction between exposure and concentration occurred (F = 0.352, df 2.83, P > 0.35).
Adult experiences with the test substance, however, did not enhance their intake during subsequent trials (F = 0.733, df 1.35, P > 0.35). As with the other cohorts, there was a test substance concentration effect (F = 30.310, df 2.35, P < 0.0001) but no exposure by concentration interaction (F = 0.884, df 2.35, P > 0.35).
Applicant's summary and conclusion
- Conclusions:
- Mice restricted their intake of all concentrations of the test substance regardless of treatment during gestation. This result is consistent with the notion that the test substance is avoided by mice at concentrations ranging from 0.25-1.0%. Nevertheless, the data also demonstrate that prenatal experience with the test substance was associated with increased ingestion of the test substance.
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