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EC number: 909-034-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-02-11 to 2010-02-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of cyclododecanol and cyclododecanone
- EC Number:
- 909-034-9
- Molecular formula:
- C12H24O (Cyclododecanol) C12H22O (Cyclododecanone)
- IUPAC Name:
- Reaction mass of cyclododecanol and cyclododecanone
- Details on test material:
- - Name of test material (as cited in study report): Cyclododecanol tel quel
- Physical state: solid
- Composition of test material, percentage of components: 65 % - 85 % cyclododecanol; 15 % - 35 % cyclododecanone
- Lot/batch No.: 3347-27129
- Expiration date of the lot/batch: 2010-10-05
- Storage condition of test material: room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 1.0 (below detection limit), 2.2 (2.0), 4.84 (4.0), 10.65 (9.1), 23.43 (22.3) mg/L nominal (measured)
- Sampling method: samples drawn from additional beakers without daphnia, kept under the same experimental conditions
- Sample storage conditions before analysis: samples were drawn, immediately transferred to the analytical laboratory and analyzed on the same day
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test substance concentrations were individually prepared in M4 medium, stirred for 24 h, and afterwards the preparations were filtered .
- Controls: were preparations without test substance treated otherwise the same way
- Evidence of undissolved material (e.g. precipitate, surface film, etc): precipitate removed by filtration
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna, water flea
- Source: Landesumweltamt NRW, Düsseldorf, Germany
- Age at study initiation (mean and range, SD): < 24 h
- Method of breeding: The daphnids were held in beakers with a nominal capacity of 2000 mL. Each beaker was filled with approximately 1400 mL M4 medium (Elendt-Schneider). Between 15 and 20 adult organisms were held in one beaker in order to get a high reproduction rate and viable offspring. The breeding beakers were kept at an additional artificial light-dark cycle of 16 to 8 hours. Breeding beakers were placed in a temperature controlled laboratory at 20 +/- 2°C. The breeding medium was renewed three times a week.
- Feeding during test: no feeding during experimental period
ACCLIMATION
- Acclimation period: culture of daphnids permanently kept at the test facility
- Acclimation conditions (same as test or not): no acclimation necessary
- Type and amount of food: singel cell green algae (Desmodesmus subspicatus)
- Feeding frequency: daily
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.2 - 3.2 mmol/L
- Test temperature:
- 20.5 - 21.8 °C
- pH:
- 7.87 - 8.06
- Dissolved oxygen:
- 5.5 - 5.9 mg/L
- Nominal and measured concentrations:
- 1.0 (below detection limit), 2.2 (2.0), 4.84 (4.0), 10.65 (9.1), 23.43 (22.3) mg/L nominal (measured)
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glas test beakers (50 mL), filled with 30 mL
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 5
- No. of vessels per control (replicates): 5
- No. of vessels per vehicle control (replicates): 5
- Biomass loading rate: 1 daphnid per 6 mL test solution
TEST MEDIUM / WATER PARAMETERS
- Total organic carbon: 0.5 mg/L
- Particulate matter: none
- Alkalinity: 0.5 - 1.5 mmol/L
- Conductivity: 536 µS/cm
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: 16 h / 8 h light / dark cycle
- Light intensity: 700 +/- 50 Lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study
- Test concentrations: 0.1 mg/L, 1 mg/L, 10 mg/L, 100 mg/L
- Results used to determine the conditions for the definitive study: immobility values of pre-test - Reference substance (positive control):
- yes
- Remarks:
- reference substance potassium dichromate was tested in an independent experiment in august 2009 and adequate sensitivity of the culture was demonstrated
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.87 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.77 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: no
- Mortality of control: no immobility observed in the control group
- Other adverse effects control: no
- Abnormal responses: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Mortality: immobility
- EC50/LC50: EC50 (24h) = 0.99 mg/L; in the required range of 0.6 - 2.1 mg/L
- Other: - Reported statistics and error estimates:
- EC values were calculated using ToxRat Professional (ToxRat Solutions GmbH, Alsdorf, Germany)
Any other information on results incl. tables
Table:EC values (probit analysis using linear max. likelihood regression) and NOEC values (Fisher`s Exact Binomial Test with Bonferroni correction)after 48 hours exposure of Daphnia magna to different concentrations of test substance Cyclododecanol tel quel
|
Effective concentration after 48 hours [mg/L] |
|
|
nominal |
analytically measured |
EC50 |
5.77 |
4.87 |
95 % Confidence interval |
0.84 – 96.25 |
n.d. |
NOEC |
1.00 |
- |
n.d.: not determined due to mathematical reasons or inappropriate data
Vessel No. |
Concentration of test substance (mg/L) |
Immobilized daphnia per test vessel |
||||||||||
|
|
24 hours |
48 hours |
|||||||||
1 - 5 |
0 (Control) |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
|
6 - 10 |
1 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
0 |
0 |
|
11 - 15 |
2.2 |
0 |
0 |
0 |
0 |
0 |
1 |
1 |
2 |
2 |
1 |
|
16 - 20 |
4.84 |
0 |
0 |
0 |
0 |
0 |
3 |
2 |
2 |
3 |
2 |
|
21 - 25 |
10.65 |
0 |
0 |
0 |
0 |
0 |
2 |
3 |
2 |
2 |
3 |
|
26 - 30 |
23.43 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (48h), based on measured concentrations, was determined as 4.87 mg/L, indicating that the test substance may be toxic to aquatic invertebrates.
- Executive summary:
The test substance Cyclododecanol tel quel was tested in the Acute Daphnia Toxicity Test according to the OECD Guideline for the Testing of Chemicals No. 202 (April 2004) using Daphnia magna (Straus) as test organism. The experiment was carried out as a static test, i.e. the test substance preparations were not exchanged for the whole exposure period of 48 hours. The test was performed with test substance preparations of nominal 1*; 2.2; 4.84; 10.65; 23.43 mg test substance/L M4 medium. Geometric mean values for analytically determined concentrations were 2.0, 4.0, 9.1, and 22.3 mg/L . The EC50value after 48 hours of exposure was 5.77 mg/L and the NOEC was 1 mg/L, based on nominal concentrations. Based on analytically determined concentrations, the EC50value was 4.87 mg/L.
*1.0 mg/L was excluded from analysis because the concentration was in the range of the limit of quantification (LOQ: 1.0 mg/L) as shown in a NON-GLP pre-test at the test site AQura GmbH.
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