tert-butyl chloroacetate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Acute toxicity data submissions

Author:

Parent, R.A.

Year:

2000

Bibliographic source:

International Journal of Toxicology, 19(5):331-373

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

not specified

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

tert-butyl chloroacetate

EC Number:

203-506-1

EC Name:

tert-butyl chloroacetate

Cas Number:

107-59-5

Molecular formula:

C6H11ClO2

IUPAC Name:

tert-butyl chloroacetate

Test material form:

liquid

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 189 g (m), 173 g (f)

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

unchanged (no vehicle)

Doses:

100, 200, 315, 350, 500, 630 and 1000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: presumably daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross pathological changes

Results and discussion

Effect levels

Effect levels 1

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

418 mg/kg bw

Based on:

test mat.

Effect levels 2

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

380 mg/kg bw

Based on:

test mat.

Mortality:

100-315 mg/kg bw: 0%
350 mg/kg bw: 30%
500 mg/kg bw: 80%
630-1000 mg/kg bw: 100%

Clinical signs:

other: Clinical signs at 200 mg/kg bw and above included piloerection, nasal discharge, bloody muzzle, increased salivation, pallor, sunken flanks, sedation, intermittent respiration, diarrhea and lateral position. Onset of symptoms was 1 h post-administration.

Gross pathology:

At necropsy dead animals had reddened lungs and mucosa of stomach and intestine. Stomach and small intestine of some animals were dilated and partly filled with watery fluid. In some animals abdominal and thoracic cavity were filled with fluid. In addition some animals displayed distinct lobulation of the liver. No abnormal findings were seen at final necropsy.

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

The approximate acute oral LD50 for males was 418 mg/kg bw and for females 380 mg/kg bw.