4,5-dichloro-2-nitroaniline

Endpoint summary

Description of key information

Short-term toxicity to aquatic invertebrates

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic invertebrate predicted for 4,5-Dichloro-2-nitroaniline(CAS: 6641-64-1). Lethal concentration i.e LC50 value estimated to be 1.23 mg/l for Daphnia magna for 48 hrs duration. It can be concluded that the 4,5-Dichloro-2-nitroaniline(CAS: 6641-64-1) is likely to be toxic to aquatic invertebrate, hence it can be considered to be “aquatic chronic 2" as per the CLP classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic algae predicted for 4,5-Dichloro-2-nitroaniline (CAS: 6641-64-1). Effect concentration i.e EC50 value estimated to be 5.3 mg/l for Desmodesmus subspicatus for 72 hrs duration. It can be concluded that the 4,5-Dichloro-2-nitroaniline (CAS: 6641-64-1) is likely to be toxic to aquatic algae, hence it can be considered to be“aquatic chronic 2" as per the CLP classification criteria for aquatic environment.

Additional information

Short-term toxicity to aquatic invertebrates

Following studies include the predicted data and experimental study for the target chemical and structurally similar read across to conclude the toxicity extent of 4,5-Dichloro-2-nitroaniline(CAS: 6641-64-1)) towards aquatic invertebrate is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic invertebrate predicted for 4,5-Dichloro-2-nitroaniline(CAS: 6641-64-1). Lethal concentration i.e LC50 value estimated to be 1.23 mg/l for Daphnia magna for 48 hrs duration. It can be concluded that the 4,5-Dichloro-2-nitroaniline(CAS: 6641-64-1) is likely to be toxic to aquatic invertebrate, hence it can be considered to be “aquatic chronic 2" as per the CLP classification criteria for aquatic environment.

Similar predicted data for the target chemical indicates tahte theOn the basis of EPI suite, ECOSAR version 1.1,the LC50 value for short term toxicity to invertebrate was predicted to be 1.43 mg/l for 4,5-dichloro-2-nitroaniline in 48 hrs. Based on the LC50 value, it can be concluded that the substance 4,5-dichloro-2-nitroaniline as toxic to aquatic environment,hence it can be considered to be “aquatic chronic 2" as per the CLP classification criteria for aquatic environment.

The above predicted data of target cheimcal is supported by the experimental studystructurally similar read across2,4,5-trichloroaniline (CAS: 636-30-6) from the publication Chemosphere 1998, suggests that the Short-term toxicity to aquatic invertebrates test was carried out according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) to study the effects of 2,3,6-trichlorophenol. The Effective concentration EC50 to 50% ofDaphnia magna for 2,4,5-trichloroaniline at 24 hr is2.2 mg/l at 24 hrs. Intoxication immobile effects was measured during the test. It can be concluded that the 2,4,5-trichloroaniline is toxic to the static environment and hence it can be considered to be “aquatic chronic 2" as per the CLP classification criteria for aquatic environment.

Further it is supported bythe experimental study anotherstructurally similar read across2,3,5-trichlorophenol (CAS: 933-78-8) from publication Bulletin of Environmental Contamination and Toxicology 1986 inidicates that the Short-term toxicity to aquatic invertebrates tests was carried out for 24 Hour under static condition to study the effects of 2,3,5-trichlorophenol on aquatic invertebrate.The Effective concentrationEC50 to 50% ofDaphnia magna is 2.28 mg/l at 24 hr for 2,3,5-trichlorophenol. It can be concluded that the 2,3,5-trichlorophenol is toxic to aquatic environment andhence it can be considered to be “aquatic chronic 2" as per the CLP classification criteria for aquatic environment.

Thus based on the effect concentrations which is in the range 1.23 mg/l to2.28 mg/lgive the conclusion that test substance4,5-Dichloro-2-nitroaniline(CAS: 6641-64-1)is likely to be toxic to aquatic invertebrate at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be “aquatic chronic 2" as per CLP classification criteria for aquatic environment.

Toxicity to aquatic algae and cyanobacteria

Following studies include the predicted data and experimental study for the target chemical and structurally similar read across to conclude the toxicity extent of 4,5-Dichloro-2-nitroaniline(CAS: 6641-64-1)) towards aquatic algae is summarized as follows:

Based on the prediction done using the OECD QSAR toolbox version 3.3 with log kow as the primary descriptor and considering the closest read across substances, the toxicity on aquatic algae predicted for 4,5-Dichloro-2-nitroaniline (CAS: 6641-64-1). Effect concentration i.e EC50 value estimated to be 5.3 mg/l for Desmodesmus subspicatus for 72 hrs duration. It can be concluded that the 4,5-Dichloro-2-nitroaniline (CAS: 6641-64-1) is likely to be toxic to aquatic algae, hence it can be considered to be“aquatic chronic 2" as per the CLP classification criteria for aquatic environment.

Similar predicted data for the target chemical indicates tahte the On the basis of EPI suite, ECOSAR version 1.1, the LC50 value for short term toxicity to aquatic algae was predicted to be 1.15 mg/l for 4,5-Dichloro-2-nitroaniline (CAS: 6641-64-1) in 96 hrs. Based on the LC50 value, it can be concluded that the substance 4,5-Dichloro-2-nitroaniline (CAS: 6641-64-1) can be considered as toxic to aquatic environment and hence can be considered to be “aquatic chronic 2" as per the CLP classification criteria for aquatic environment.

The above predicted data of target cheimcal is supported by the experimental studystructurally similar read across2,4,5-trichloroaniline (CAS: 636-30-6) from the publication Aquatic Toxicology 1999, suggests that the Effects on the population growth inhibition of algae was tested according to the OECD guidelines for 2,4,5-trichloroaniline under static condition. The Effective concentration EC50 to 50% ofChlorella pyrenoidosa at 72 hr is 3 mg/l. It can be concluded from the value that the 2,4,5-trichloroaniline is not toxic to the aquatic algae and can be considered as “aquatic chronic 2” as per classification criteria for aquatic invertebrate.

Further it is supported bythe experimental study anotherstructurally similar read across2,4-xylidine(CAS: 95 -68 -1), from the publication Water Research 1980 indicates that the cell multiplication inhibition test was carried out for 72 hrs to study the effects of 2,4-xylidine on aquatic algae.

The lowest-observed-effect concentration (LOEC) to Scenedesmus quadricauda in 72 hrs is 9.8 mg/l. cell multiplication inhibition was measured during the test. It can be concluded that the 2,4-xylidine is toxic to the aquatic environment and hence from the value it can be considered as “aquatic chronic 2” as per the CLP classification criteria.

Thus based on the effect concentrations which is in the range 1.15 mg/l to 9.8mg/lgive the conclusion that test substance4,5-Dichloro-2-nitroaniline(CAS: 6641-64-1)is likely to be toxic to aquatic algae at environmentally relevant concentrations and applying weight of evidence approach it can be considered to be “aquatic chronic 2" as per CLP classification criteria for aquatic environment.