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EC number: 210-283-4 | CAS number: 611-92-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02.11.-02.12.2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- anaerobic
- Inoculum or test system:
- sewage, predominantly domestic, non-adapted
- Details on inoculum:
- The inoculum contained a mixed population of microorganisms obtained from the secondary effluent of the waste water treatment plant of Pardubice processing predominantly municipal sewage.
PREPARATION OF INOCULUM
The fresh collected waste water was filtered through paper filter. COD was determined and waste water was aerated till using.
Justification of the test system
The inoculum preparation is in conformity with the recommendations of the test guideline. - Duration of test (contact time):
- 28 d
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Test temperature: 20+/-1 °C
- pH of medium: 7.4
- pH adjusted: no
- Illummination: in the dark
PREPARATION OF SOLUTIONS FOR THE TEST
- Inoculated medium: TThe volume of waste water for the inoculation of mineral medium was chosen 1.9 mL per 1 L of medium (COD of waste water: 71 mg·L-1). 30 L aerated mineral medium was prepared for the test and before the beginning of the test it was inoculated with 57 mL of modified waste water. The pH value of medium: 7.5.
- Test substance: The stock solution of the test substance was prepared in concentration 0.1001 g·L-1
of deionized water. Because the test substance was poorly soluble, the stock solution was stirred for period 24 hours for achieving the highest solubility.
From this stock solution 120 mL (20 mL·L-1) were transferred to a large glass bottle and completed with the inoculated medium to the volume of 6 L.
The solution prepared in this way contained 2.00 mg·L-1 of the test substance. The pH value of solution: 7.3.
TEST SYSTEM
Test substance series: 2 x 9 bottles with test substance and inoculum
Reference substance series: 2 x 9 bottles with reference substance and inoculum
Blank series: 2 x 9 bottles with inoculated medium only
Toxicity test series: 2 x 5 bottles with test substance, reference substance and inoculum
Series for nitrification determination: 1 x 9 bottles with test substance and inoculum
The prepared dosing solutions for each series was filled in two parallel bottle replicates (except series for nitrification determination).
The bottles were placed into thermostat.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, the inoculated mineral medium
- Toxicity control: yes, the test and reference substance mixture
Measurement: At the zero-time and at the 3rd, 7th, 10th, 14th, 17th, 21st, 24th and 28th day of test the
appropriate bottles from each series were taken off and they were analysed for dissolved oxygen by the Winkler method. - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 69.2
- Sampling time:
- 28 d
- Key result
- Parameter:
- BOD5
- Value:
- 0.16 other: mg/mg
- Key result
- Parameter:
- COD
- Value:
- 2.088 other: mg/mg
- Parameter:
- ThOD
- Value:
- 2.796 other: mg/mg
- Remarks on result:
- other: with total nitrification
- Parameter:
- ThOD
- Value:
- 2.264 other: mg/mg
- Remarks on result:
- other: without total nitrification
- Results with reference substance:
- COD of reference substance 1.640 mg.mg-1 and 85.9 %
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- In this 28-day study of ready biological degradability the degradation of 69.2 % of the test substance, Methylcentralit, was attained in the end of study.
- Executive summary:
The test substance, Methylcentralit,was tested forthe ready biological degradability in Closed Bottle Test.
Test performance
The test was performed according to:
Method C.4E - Closed Bottle Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
The results of biological degradation are related to experimentally determined COD values of the test and reference substance at the beginning of the test.
The test substance was soluble in used mineral medium. The stock solution of the test substance was stirred for period 24 hours and then the dosage from the stock solution was carried out.
Sodium benzoate was used as the reference substance. The dosage was carried out from the stock solution.
Specific COD of the test substance in medium: 2.088 mg·mg-1
Specific COD of the reference substance in medium: 1.640 mg·mg-1
In parallel to the main test the toxicity test was performed.
Based on the test substance contains nitrogen in the chemical composition, the oxidized nitrogen forms were determined and the correction for nitrification was carried out.
The test was performed at temperature of 20 ± 1 °C with the pH values of solutions 7 – 8
at the beginning of the test.Validity of the test
The prescribed validity criteria in the test were fulfilled.
The test substance was not inhibiting for the used inoculum.
Since all criteria of acceptability were met, this study is considered to be valid.
Test results
In this28-day study of ready biological degradabilitythe degradation of 69.2 % of the test substance,Methylcentralit,was attained in the end of study.
Reference
Description of key information
Only one study is available.
The test was performed according to: Method C.4E - Closed Bottle Test, Council Regulation (EC) No. 440/2008, published in O.J.L 142, 2008.
GLP study.
Klimish score 1.
The test substance, Methylcentralit, was tested for the ready biological degradability in Closed Bottle Test.
Based on results of this 28 days study Methylcentralit is not ready biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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