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EC number: 232-340-2 | CAS number: 8005-56-9 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 71110.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Study meets requirements of OECD Guideline 401, 84/449/EEC, B.1 without deviations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Bisbenzimidazobenzo[lmn][3,8]phenanthrolinedione
- EC Number:
- 232-340-2
- EC Name:
- Bisbenzimidazobenzo[lmn][3,8]phenanthrolinedione
- Cas Number:
- 8005-56-9
- Molecular formula:
- C26H12N4O2
- IUPAC Name:
- bisbenzimidazobenzo[lmn][3,8]phenanthrolinedione
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Strain specifics: Hoe: WISKf (SPF71)
- Source: Hoechst AG, Kastengrund, SPF-breed
- Age at study initiation: 7 weeks (male), 8 weeks (female)
- Weight at study initiation: male 170 g - 183 g (mean 177 g), female 160 g - 175 g (mean 167 g)
- Fasting period before study: approximately 16 hours before until 3-4 hours after treatment, access to water permitted
- Housing: in groups of five in Makrolon type 4 cages with standard softwood bedding
- Diet (e.g. ad libitum): standard rat diet (Altromin 1324) ad libitum
- Water (e.g. ad libitum): tap water in plastic bottles ad litidum
- Acclimation period: not necessary (breeding at identical conditions)
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 3°C
- Humidity (%): 50 ± 20 %
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: sesame oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 20.0 % suspension; the test item was suspended in sesame oil and distributed homogeneously by means of a magnetic stirrer. Stability and homogeneity of the test substance was determined by analytical methods and guaranteed for 4 hours.
- Amount of vehicle (if gavage): 10 mL/kg body weight (test item in vehicle administered)
- Purity: Oleum Sesami Ph. Eur. III - Doses:
- 2000 mg/kg body weight
- No. of animals per sex per dose:
- 5 males
5 females - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 15 days starting with treatment day 1
- Frequency of observations and weighing:
- symptoms were recorded twice daily, on weekends and holidays only once.
- body weights: on days 1 (prior to administration), 8 and 15
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, macroscopic examination - Statistics:
- None
Results and discussion
Effect levels
- Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- no deaths occured during the whole study
- Clinical signs:
- other: no symptoms were observed after application of 2000 mg/kg bw. One day p.a. the feces of the animals were discoloured red.
- Gross pathology:
- No macroscopic findings at scheduled necropsy.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Remarks:
- The test item has not to be classified for acute oral toxicity according to Regulation (EC) No 1272/2008
- Conclusions:
- The test item has not to be classified for acute oral toxicity according to Regulation (EC) No 1272/2008.
- Executive summary:
One group of five male HoeWISK (SPF71) rats and one group of five female HoeWISK (SPF71) rats were treated with the test item by oral gavage administration at a dosage of 2000 mg/kg body weight. The test item was suspended in vehicle (sesame oil) at a concentration of 0.2 g/mL and administered at a volume dosage of 10 mL/kg.
The animals were examined for mortality and clinical signs twice daily (once at weekends and holidays) during test days 2-15. Body weights were recorded on day 1 (prior to administration) and on days 8 and 15. All animals were necropsied and examined macroscopically.
After application of 2000 mg/kg b.w. neither deaths nor symptoms occurred leading to an LD50 above 2000 mg/kg bw.
One day after application the feces of the animals was discoloured red.
Development of body weight was not impaired.
The animals killed at the end of the observation period showed no macroscopically visible changes
Based on that findings, the test item has not to be classified for acute oral toxicity according to Regulation (EC) No 1272/2008.
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