Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 264-598-7 | CAS number: 64001-15-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2 June 1992 to 5 June 1992
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to ISO 8192 in compliance with GLP. The reliability was set on 2 as no blank was included and results of reference substance are not stated.
- Justification for type of information:
- The information is used for read across to Cyclacet Dihydro.
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- not specified
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Source: Laboratory Waste Water purification plant (according to OECD confirmatory test for surfactants). Concentration of inoculum was 6 g/L dry matter.
- Test type:
- other: respiration measurement (O2-decrease) within the testing period
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Nominal and measured concentrations:
- 100, 180, 320, 560, 1000 mg/l
- Details on test conditions:
- TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Activated sludge was mixed with an artificial, standardized wastewater. The diluted test material is added.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The decrease of respiration is measured. - Reference substance (positive control):
- yes
- Remarks:
- 3,5 Dichlorophenol (CAS 25167-81-1)
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 245 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- 53 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: read from concentration/effect curve
- Details on results:
- Test concentration [mg/L] versus (vs) Inhibition [%]
100 vs 26.2
180 vs 35.1
320 vs 57.4
560 vs 77.1
1000 vs 88.7 - Results with reference substance (positive control):
- Test data have been confirmed by the reference substance (raw data).
- Reported statistics and error estimates:
- The EC 50 value was determined graphically from the relation between concentration and inhibition effect (semilogarithmic scale)
- Validity criteria fulfilled:
- no
- Remarks:
- no blank included, results of reference substance not stated, test period of results not stated
- Conclusions:
- An ASRI EC50 value of 245 mg/L was determined in a study conducted according to an appropriate test protocol, and in compliance with GLP.
- Executive summary:
An activated sludge respiration inhibition test with Cyclacet has been performed, according to ISO 8192, which is similar to OECD TG 209. The sludge concentration was 6 g/l. The nominal test concentrations were 100, 180, 320, 560 and 1000 mg/l. A dose related increase in inhibition was seen from 26 to 88 mg/l. The 30min-EC50 and 30min-EC10 were found to be 245 and 53 mg/l respectively.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The information is based on read across
- Justification for type of information:
- The information on Cyclacet Dihydro is derived from the analogue Cyclacet. The full read across is presented in the Endpoint summary. The accompaning file is also attached there.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- 245 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- 53 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Validity criteria fulfilled:
- yes
Referenceopen allclose all
purity of the test substance not stated, test period of results not stated, results of reference substance not stated, no blank included
Description of key information
The acute, EC50, and chronic, EC10, for micro-organisms is 245 and 53 mg/l, based on read across information of Cyclacet tested in an OECD TG 209 study.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 245 mg/L
- EC10 or NOEC for microorganisms:
- 53 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.