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EC number: 625-309-3 | CAS number: 6574-97-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
In order to evalute the acute oral toxic potential of 2,3 -Dichlorobenzonitrile a study according to OECD 425 and OPPTS 871 .1100 was performed.
Under the conditions of the present study the estimated LD50 of the test item 2,3-Dichlorobenzonitrile in rats is 550 mg/kg bw.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 14.01.- 14.06.2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 425 (Acute Oral Toxicity: Up-and-Down Procedure)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.1100 (Acute Oral Toxicity)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- up-and-down procedure
- Limit test:
- no
- Specific details on test material used for the study:
- Batch-No.: 529301
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River, 97633 Sulzfeld, Germany
- Females nulliparous and non-pregnant: yes
- Age at study initiation: 8 - 12 weeks
- Weight at study initiation:150 - 184 g
- Fasting period before study: 16 - 19 hours
- Housing: The animals were kept in groups in IVC cages, type III H, polysulphona cages on Altromin saw fibre beeding.
- Diet: Free access to Altromin 1324 maintenance diet for rats and mice (lot. no. 2922)
- Water: Free access to tap water, sulphur acified to a pH value of approximately 2.8 (drinking water, municipal residue control, microbiological controls at regular intervals)
- Acclimation period: At least five days under laboratory conditions.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 3 °C
- Humidity (%): 55 +/- 10 %
- Air changes (per hr): 10 x/ hour
- Photoperiod: Artificial light, sequence being 12 hours light, 12 hours dark.
- Route of administration:
- oral: gavage
- Vehicle:
- corn oil
- Details on oral exposure:
- VEHICLE
- Amount of vehicle (if gavage): 10 mL/kg body weight
- Justification for choice of vehicle: While aqua ad injectionem (sterile water) was not adequate, preparation with corn oil yielded a suspension which was technically applicable to the animals.
- Lot/batch no.: 529301
- Purity: 99.7 %
MAXIMUM DOSE VOLUME APPLIED:
10 mL/kg body weight - Doses:
- 2000, 550 and 175 mg/kg body weight
- No. of animals per sex per dose:
- 2000 mg/kg bw: 4 females
550 mg/kg bw: 4 females
175 mg/kg bw: 2 females - Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: A careful examination was made several times on the day of dosing (at least once during the first 30 minutes and with special attention given during the first 4 hours post-dose). As soon as symptomy were noticed they were recorded. Thereafter, the animals were observed for clinical signs once daily until the end of the observation period. All abnormalities were recorded. Cageside observations included changes in the skin and fur, eyes and mucous membranes. Also respiratory, circulatory, automic and central nervous systems and somatomotor activitiy and behaviour pattern were examined. Particular attention was directed to observations of tremor, convulsions,salivation, diarrhoea, lethargy, sleep and coma.
- Frequency of weighing: The animals were weighed on day 1 (prior to the administration) and on days 8 and 15.
- Necropsy of survivors performed: yes - Key result
- Sex:
- female
- Dose descriptor:
- LD50 cut-off
- Effect level:
- 550 mg/kg bw
- Based on:
- test mat.
- Mortality:
- All animals treated with the test item at a dose of 2000 mg/kg bw and one animal treated with the test item at a dose of 550 mg/kg had to be sacrified for ethical reasons on test day1. All remaining animals survived until the end of the study.
- Clinical signs:
- other: The most relevant clinical findings in the animals treated with the test item at a dose of 2000 mg/kg bw were reduced spontaneous activity, apathy, prone position, ataxia, slow movements, wasp waist, eyes half closed, eyes closed, abnormal breathing, hypo
- Gross pathology:
- With the expection of acute injection of blood vessels in the abdominal region, which is due to the euthanasia injection, no specific gross pathological changes were recordedfor any other animal. At necropsy, in the stomach of animal 1 (2000 mg/kg bw) residual test item was found.
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- Under the conditions of the present study and according to OECD Guideline 425 and OPPTS 871.1100 the estimated LD50 of the test item 2,3-Dichlorobenzonitrile in rats is 550 mg/kg bw (based on an assumed sigma of 0.5).
- Executive summary:
In order to evalute the acute toxic potential of 2,3 -Dichlorobenzonitrile a study according to OECD 425 and OPPTS 871 .1100 was performed.
All animals treated with the test item at a dose of 2000 mg/kg bw and one animal treated with the test item at a dose of 550 mg/kg had to be sacrified for ethical reasons on test day1. All remaining animals survived until the end of the study.
The most relevant clinical findings in the animals treated with the test item at a dose of 2000 mg/kg bw were reduced spontaneous activity, apathy, prone position, ataxia, slow movements, wasp waist, eyes half closed, eyes closed, abnormal breathing, hypothermia, lacrimation and comatose. The most relevant clinical findings in the animals treated with the test item at a dose of 550 mg/kg bw were reduced spontaneous activity, prone position, hunched posture, ataxia, slow movements, moving the bedding, wasp waist, eyes half closed, piloerection, abnormal breathing, lacrimation and salivation. The most relevant clinical findings in the animals treated with the test item at a dose of 175 mg/kg bw were reduced spontaneous activity, hunched posture, slow movements, wasp waist, piloerection, eyes half closed and eyes closed.
All surviving animals gained weight during the observation period. Througout the 14-day observation period, the weight gain of surviving animals was within the normal range of variation for this strain.
With the expection of acute injection of blood vessels in the abdominal region, which is due to the euthanasia injection, no specific gross pathological changes were recorded for any other animal. At necropsy, in the stomach of animal 1 (2000 mg/kg bw) residual test item was found.
Under the conditions of the present study and according to OECD Guideline 425 and OPPTS 871.1100 the estimated LD50 of the test item 2,3-Dichlorobenzonitrile in rats is 550 mg/kg bw (based on an assumed sigma of 0.5).
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 550 mg/kg bw
Additional information
Justification for classification or non-classification
Under the conditions of the present study and according to OECD Guideline 425 and OPPTS 871.1100 the estimated oral LD50 of the test item 2,3-Dichlorobenzonitrile in rats is 550 mg/kg bw.
On the basis of the test results and the criteria given in GHS (Globally Harmonized System of Classification and Labelling of Chemicals) the substance 2,3 -Dichlorobenzonitrile should be classified into category 4 and labelled with H302: Harmful if swallowed.
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