Dimethyl vinylphosphonate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

September 1984 and October 1984

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Report date:

1984

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Version / remarks:

1981

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method B.1 (Acute Toxicity (Oral))

Version / remarks:

1979

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl vinylphosphonate

EC Number:

225-076-4

EC Name:

Dimethyl vinylphosphonate

Cas Number:

4645-32-3

Molecular formula:

C4H9O3P

IUPAC Name:

Dimethyl ethenylphosphonate

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: HOECHST AG, Kastengrund
- Weight at study initiation: 164 - 205 g (males); 162-193 g (females)
- Fasting period before study: 16 hours before application
- Housing: in groups of 5 in macrolon cages
- Diet: ad libitum (Altromin 1324)
- Water: ad libitum (tap water)
- Acclimation period: at least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2
- Humidity (%): 55 +/- 10
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 25% (w/v)

MAXIMUM DOSE VOLUME APPLIED: 5.0 mL/kg bw

DOSAGE PREPARATION: The test item was diluted in water to a concentration of 25% (w/v).

Doses:

315, 500, 560, 630, 800, 1000 and 1250 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: weekly
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, macroscopically examinations

Statistics:

- Probit analysis according to Lindner and Weber
- method according to Fieller or Sidak

Results and discussion

Preliminary study:

No details on preliminary study.

Effect levels

Effect levels 1

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

858 mg/kg bw

Based on:

test mat.

Effect levels 2

Key result

Sex:

female

Dose descriptor:

LD50

Effect level:

602 mg/kg bw

Based on:

test mat.

Mortality:

630 mg/kg bw: 3/5 (females)
800 mg/kg bw: 2/5 (males); 5/5 (females)
1000 mg/kg bw: 4/5 (males)
1250 mg/kg bw: 5/5 (males)

Clinical signs:

Clinical signs were observed in male and female rats. On day of application occured squatting position, draftee flanks, tumbling gait, calm behaviour, pilorection. One day after application prone position, trembling, tonic cramping, jumping cramps, blood coloured lacrimation, blood stained snouts and increased breathing frequency were observed. One male had temporarily light brown-coloured decay.

Body weight:

No changes were observed.

Gross pathology:

The decendents animals showed the following findings: hemorrhages in the stomach, stomach content was darkly coloured (brown), small intestine filled with yellow or reddish brown thin fluid mass, lungs filled with blood, lung was bright red, spleen was lightened, spleen was strongly diminished, pancreas was dark red and both kidneys were lightened. In one high dose male animal, liver was strongly diminished. The stomach of this animal was deformed, the urinary bladder bulging, both adrenal glands darkened, and the epididymis reddish. Animals killed at the end of the observation period were free of macroscopically visible changes.

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

The LD50 value of the test item was determined to be 858.0 mg/kg bw in males and 602 mg/kg bw in females.

Executive summary:

The acute oral toxicity of the test item was investigated in a study according to OECD TG 401 and EU Method B.1. Five animals each were exposed to the test item by oral gavage at doses ranging from 315 ranging from 1250 mg/kg bw. Animals were observed for 14 days. Mortality occured in 3 of 5 females at 630 mg/kg bw, in 2 of 5 males and 5 of 5 females at 800 mg/kg bw, 4 of 5 males at 1000 mg/kg bw and 5 of 5 males at 1250 mg/kg bw. Clinical were observed in both male and female animals on day of application and one day after application. The body weight was not affected. The decendents animals showed the following macrosopic findings. Animals killed at the end of the observation period were free of macroscopically visible changes. Based on the available data, the LD50 value of the test item was determined to be 858.0 mg/kg bw in males and 602 mg/kg bw in females.