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EC number: 204-582-9 | CAS number: 122-91-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from publication.
Data source
Reference
- Reference Type:
- publication
- Title:
- Evaluation of the potential effects of ingredients added to cigarettes. Part 3: In vitro genotoxicity and cytotoxicity*
- Author:
- E. Roemera, F.J. Tewesa, T.J. Meisgena, D.J. Veltela, E.L. Carminesb
- Year:
- 2 002
- Bibliographic source:
- Food and Chemical Toxicology 40 (2002) 105–111
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Principles of method if other than guideline:
- In vitro genotoxicity study of cigarette smoke (Anisyl Formate) by using Salmonella plate incorporation (Ames) assay.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Anisyl formate
- EC Number:
- 204-582-9
- EC Name:
- Anisyl formate
- Cas Number:
- 122-91-8
- Molecular formula:
- C9H10O3
- IUPAC Name:
- (4-methoxyphenyl)methyl formate
- Test material form:
- liquid
- Details on test material:
- Details on test material
- Name of test material (as cited in study report): Anisyl Formate
- Molecular formula: C9H10O3
- Molecular weight: 166.175 g/mole
- Substance type: Organic
- Physical state: Liquid
Constituent 1
- Specific details on test material used for the study:
- Details on test material
- Name of test material (as cited in study report): Anisyl Formate
- Molecular formula: C9H10O3
- Molecular weight: 166.175 g/mole
- Substance type: Organic
- Physical state: Liquid
Method
- Target gene:
- Histidine
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100, TA102, TA1535 and TA1537.
- Details on mammalian cell type (if applicable):
- Not applicable.
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- Not applicable.
- Metabolic activation:
- with and without
- Metabolic activation system:
- Metabolic activation system consisting of the postmitochondrial fraction of the livers from rats treated with Aroclor 1254
- Test concentrations with justification for top dose:
- 0, 1, 2, 3, 4, 5 and 5 mg/plate
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 2-acetylaminofluorene
- Details on test system and experimental conditions:
- Details on test system and conditions
METHOD OF APPLICATION: In agar (plate incorporation)
DURATION
- Preincubation period: No data available
- Exposure duration: 44–48 h.
- Expression time (cells in growth medium): No data available
- Selection time (if incubation with a selection agent): No data available
- Fixation time (start of exposure up to fixation or harvest of cells): No data available
SELECTION AGENT (mutation assays): No data available
SPINDLE INHIBITOR (cytogenetic assays): No data available
STAIN (for cytogenetic assays): Mouse embryo BALB/c 3T3 cells
NUMBER OF REPLICATIONS: 3
NUMBER OF CELLS EVALUATED: 5
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index; cloning efficiency; relative total growth; other: Neutral red uptake assay
OTHER EXAMINATIONS:
- Determination of polyploidy: No data available
- Determination of endoreplication: No data available
- Other: No data available
OTHER: No data available - Rationale for test conditions:
- Not specified
- Evaluation criteria:
- Number of His+ revertant colonies was determined
- Statistics:
- Arithmetic means and measures of variance were calculated as descriptive statistics. The one-way analysis of variance was used to compare the results obtained for the control cigarette and those obtained for the test cigarettes containing the same group of ingredients. In those cases where this overall comparison showed a significant difference between the cigarettes, the Duncan test (Duncan, 1955) for pairwise comparison was applied. Results were considered to be statistically significant at P40.05 without adjustment for multiple testing.
Results and discussion
Test results
- Key result
- Species / strain:
- S. typhimurium, other: TA98, TA100, TA102, TA1535 and TA1537.
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- COMPARISON WITH HISTORICAL CONTROL DATA: Yes
Applicant's summary and conclusion
- Conclusions:
- Anisyl Formate did not induce mutagenicity in an Ames assay conducted on S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102 strains with and without rat liver S9 activation.
- Executive summary:
Genetic toxicity in vitro study was assessed forAnisyl Formate(122-91-8) to evaluate its possible mutagenic potential .For this purpose AMES assay was performed according to guideline OECD 471.Anisyl Formate was exposed toS. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102 strains with and without rat liver S9 activation byplate incorporation assay at the concentration of0, 1, 2, 3, 4, 5 and 5 mg/plate. DMSO was used as solvent. Negative and positive control were used .Anisyl Formatedid not induce mutagenicity in an Ames assay conducted on S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and TA 102 strains with and without rat liver S9 activation byplate incorporation assay .Although positive response is observed in some strains, there were also no apparent trends discernible that would suggest a change in mutagenic activity caused by the addition of the ingredient chemicals. ThereforeAnisyl Formate(122-91-8) was not likely to classify as gene mutant in vitro.
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