N-ethyl-2-(isopropyl)-5-methylcyclohexanecarboxamide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 48 hrs stirring for achieving test concentrations of 100 mg/L ,respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 2.04 cm
- Weight at study initiation (mean and range, SD): average 0.084 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

185 mg of CaCO3

Test temperature:

23 °C

pH:

7.1

Dissolved oxygen:

7.0 mg/l

Nominal and measured concentrations:

100 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Mortality was observed

Sublethal observations / clinical signs:

9.1. Mortality and symptoms

Nominal Concentration	Number of fish	Mortality (cumulated) after
(mg/l)		3 h	6 h	24h	48h	72h	96h
		V1	V1	V1	V1	V1	V1
Control	8	-	-	-	-	-	-
100	8	-	-	1	2	2	2

Visible Symptoms:

Nominal Concentration	Number of fish	3 h	6 h	24h	48h	72h	96h
(mg/l)		V1	V1	V1	V1	V1	V1
Control	8	NS4	NS4	NS4	NS4	NS4	NS4
100	8	NS4	NS4	M3	M2	M2	M2

Explanation of symptoms:

 A: apathy

 N: Narcotic-like state

S: Surfacing behaviour

 C: Swimming near water surface

 M: Slow movements compared to control NS: No abnormal symptomsS: Surfacing behaviour

 L: Loss of equilibrium SM: Shimming movement H1: Hyperactivity

 H2: Hypo activity

 B: Bending behavior

 F: Fin rot

 IS: Invert Swimming

 SB: Swimming behaviour

 RF: Respiratory Function

 P: Pigmentation

 E: Exopthalamos SC: Schooling Effect

8.2. pH and oxygen content:

Nominal	Number of fish	pH readings after
		24h	48h	72h	96h
Concentration
(mg/l)
		V1	V1	V1	V1
Control	8	7.4	7.5	7.6	7.7
100	8	7.9	8.0	8.1	8.2

 

Nominal	Number of fish	Oxygen content (mg/L) after
Concentration
(mg/l)		24h	48h	72h	96h
		V1	V1	V1	V1
Control	8	6.7	6.6	6.5	6.4
100	8	6.5	6.4	6.3	6.3

9.3. Test temperature

Nominal	Number of fish		Temperature (°C) after
Concentration
(mg/l)		24h	48h	72h	96h
		V1	V1	V1	V1
Control	8	23.9	23.8	24.1	23.8
100	8	23.9	23.8	24.1	23.8

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test substance on Zebra Fish Danio rerio was determine to be 100 mg/l

Executive summary:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 48 hrs stirring for achieving test concentrations of 100 mg/L ,respectively. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations 100 mg/l, LC50 was determine to be 100 mg/l. Mortality was observed at 100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.

Description of key information

Short-term toxicity to fish:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 48 hrs stirring for achieving test concentrations of 100 mg/L ,respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations 100 mg/l, LC50 was determine to be 100 mg/l. Mortality was observed at 100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

100 mg/L

Additional information

Short-term toxicity to fish:

Study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with continuous 48 hrs stirring for achieving test concentrations of 100 mg/L ,respectively.This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L and Zebra FishDanio reriowere exposed to these concentration for 96 hours.Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations 100 mg/l, LC50 was determine to be 100 mg/l. Mortality was observed at 100 mg/l . Based on the LC50, it can be consider that the chemical was toxic and can be consider to be classified aq aquatic chronic 3 as per the CLP classification criteria.