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EC number: 205-516-1 | CAS number: 141-97-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009-11-17 to 2009-11-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
no data - Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: The limit concentration and the control were analytically verified via LC-MS/MS after 0 hours (fresh media) and after 48 hours (old media).
- Sampling method: For the old media, separate replicates without test organisms were prepared and incubated under test conditions. Recoveries of the test item should be within ± 20 % of the nominal value throughout exposure.
- Sample storage conditions before analysis:All samples were stored at room temperature, if necessary. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solution (100 mg/L test item were weighed out) was freshly prepared with dilution water prior to application. Dispersion treatment was agitation.
- Eluate: Dilution water
- Differential loading: 1000 mg/L
- Controls: Dilution water without test item tested under the same conditions as the test group.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): None - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- Lufthygiene (WaBoLu), further own breeding
- Age at study initiation (mean and range, SD): 2 to 24 h
- Feeding during test: No feeding
- Method of breeding: In 2-3 L glass vessels with appr. 1.8 L culture medium, at 20 +/- 2 °C, in an incubator, 16 h illumination, illumination strengt max. 20 µExm-2 x s-1
ACCLIMATION
- Acclimation period: 2 h
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: No feeding
- Health during acclimation (any mortality observed): Healthy, no mortality observed
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No
- Hardness:
- Dilution water at test start: 255 (mg CaCO3/L)
- Test temperature:
- 22.0 °C
- pH:
- Water Quality Parameters at the Beginning of the Test (0 hours, Fresh Media / measured in one additional replicate of the limit concentration and the control):
- Nominal Test Item Concentration 100 mg/L: pH-Value = 7.72
- Control: pH-value = 7.79
Water Quality Parameters at the End of the Test (48 hours, Old Media / measured in all replicates):
- Nominal Test Item Concentration 100 mg/L: pH-Values = 7.56-7.61
- Control: pH-value = 7.51-7.62 - Dissolved oxygen:
- Water Quality Parameters at the Beginning of the Test (0 hours, Fresh Media / measured in one additional replicate of the limit concentration and the control):
- Nominal Test Item Concentration 100 mg/L: Dissolved O2-Concentration = 8.32 mg/L
- Control: Dissolved O2-Concentration = 8.10 mg/L
Water Quality Parameters at the End of the Test (48 hours, Old Media / measured in all replicates):
- Nominal Test Item Concentration 100 mg/L: Dissolved O2-Concentration = 7.90-8.04 mg/L
- Control: Dissolved O2-Concentration = 7.92-8.09 mg/L - Salinity:
- Freshwater
- Nominal and measured concentrations:
- Nominal Test Item Concentrations of 100 mg/L corresponds to measured concentrations (mean value of 2 injections - dilution factor taken into account) of 90.7 mg/L (recovery rate 91%). and 101 mg/L (recovery rate 100%). In the control samples, the measured concentrations were below the limit of quantification of the analytical method.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers, 50 mL
Type: closed with parafilm
Material, size, headspace, fill volume: Glass beakers (4 cm ID x 7 cm H), 50 mL
Aeration: No
- Renewal rate of test solution (frequency/flow rate): One application at test start, static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): None
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Acc. to Directive 92/69/EEC Method C.2, Annex 1
- Conductivity: Dilution water at test start: 640 µS/cm
- Culture medium different from test medium: Elendt M4 acc. to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L
- Intervals of water quality measurement: At test start and test end
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16:8 light:dark cycle
- Light intensity: Diffuse light, illumination strength max. 20 µE x m-2 x s-1
TEST CONCENTRATIONS
- Spacing factor for test concentrations: None, limit test
- Range finding study: Test concentrations: 10 - 100 mg/L
IMMOBILIZATION RATES OF THE PRELIMINARY RANGE-FINDING TEST [%] (n = 20, divided into 2 replicates with 10 daphnids each):
- At concentrations of 100 and 10 mg/L nominal as well as in the control samples, no immobility was noted. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: None
- Mortality of control: 0%
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50/LC50: 1.67 (CI 1.63 - 1.71) mg/L - Reported statistics and error estimates:
- The effects of the limit concentration of 1000 mg/L were determined directly from the observation data.
- Validity criteria fulfilled:
- yes
- Conclusions:
- At the limit concentration 100 mg/L (nominal) of the test item Ethyl acetoacetate, no effect was observed. The EC50 (48h) on Daphnia Magna was found to be > 100 mg/l.
- Executive summary:
In the acute immobilization test to Daphnia magna (STRAUS) the effects of the limit concentration 100 mg/L of the test item EAA were determined according to OECD 202 (2004). The limit test was conducted 2009 under static conditions over a period of 48 hours with analytical monitoring. 20 test organisms were exposed to the limit concentration and control.
At the limit concentration 100 mg/L (nominal) of the test item Ethyl acetoacetate, no effect was observed.
Reference
Percentage of Daphnids Incapable of Swimming after 24 and 48 h of Exposure:
(n = 20, divided into 4 replicates with 5 daphnids each)
IMMOBILIZATION [%] |
||||||||||
Nominal Test Item Concentration [mg/L] |
24 h |
48 h |
||||||||
Replicates |
Replicates |
|||||||||
1 |
2 |
3 |
4 |
MV |
1 |
2 |
3 |
4 |
MV |
|
100 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Description of key information
In the acute immobilization test to Daphnia magna (STRAUS) the effects of the limit concentration 100 mg/L of the test item EAA were determined according to OECD 202 (2004). The limit test was conducted 2009 under static conditions over a period of 48 hours with analytical monitoring. 20 test organisms were exposed to the limit concentration and control. At the limit concentration 100 mg/L (nominal) of the test item Ethyl acetoacetate, no effect was observed.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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