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EC number: 214-262-0 | CAS number: 1118-39-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-11-18 to 1999-12-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- GLP compliance:
- yes
- Oxygen conditions:
- not specified
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Fresh activated sludge from a biological waste water treatment plant treating predominantly domestic sewage (City of Geneva, Aire) was used.
- Preparation of inoculum for exposure: The sludge is collected in the morning, washed three times in the mineral medium (by centrifuging at 1000 g for 10 minutes, discarding the supernatant and resuspending in mineral medium) and kept aerobic until being used on the same day.
- Determination of the dry weight of suspended solids: The dry weight of suspended solids is determined by taking two 50 mL samples of the homogenised sludge, evaporating water on a steam bath, drying in an oven at 105 - 110 oC for two hours and weighing the residue.
- Dry weight of suspended solids: 3.289 g/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Water used during this study is deionised water containing less than 10 mg/L dissolved organic carbon.
- Composition of medium: Stock solutions of mineral components (solution A, B, C, D) were prepared according to the guideline. Mineral medium is prepared by mixing 50 mL of solution A and 2000 mL deionised water, adding 5 mL of each of the solutions B, C and D and making up to 5 litres with deionised water. The pH is measured and if necessary adjusted to 7.4 +/- 0.2 with phosphoric acid or potassium hydroxide.
- Test temperature: 22 oC
- pH: 7.4 (at initiation)
- Activated sludge: to obtain a concentration of 30 mg/L (dry weight) in a 250 mL flask, 2.28 mL of sludge (dry weight of suspended solids: 3.289 g/L) is needed (inoculum).
TEST SYSTEM
- Culturing apparatus: Respirometer, SAPROMAT D12, made by J. M. VOITH GmbH, Heidenheim, Germany.
- Number of culture flasks/concentration: 2/test suspension, 2/procedure control, 2/inoculum blank, 2/toxicity control
- Measuring equipment: SAPROMAT meter
- Details of trap for CO2 if used: about 2 g soda lime is placed in an attachment of the stopper of 250-mL flasks.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: Yes, the toxicity of the test chemical for the inoculum is checked. Therefore, a pair of flasks of the volumetric respirometer are filled with mineral medium + test chemical (100 mg/L) + reference substance (100 mg/L) + inoculum and their respirations are recorded as for the other flasks. If they are lower than those of the flasks containing mineral medium + referenc substance (100 mg/L) + incoculum, the test chemical can be assumed to be inhibitory to the inoculum used.
RESULTS
Oxygen uptakes, as read on the SAPROMAT meters, are corrected:
- by deducting the basic oxygen uptake of sludge
- proportionally to account for the small differences between actual and nominal concentrations of test and reference substances
Means of identical flasks were used to obtain the different results and curves. - Reference substance:
- other: Sodium Benzoate (Purity: min. 99.0%)
- Remarks:
- Test concentration: 100 mg/L
- Test performance:
- Final pH values are all surprisingly high (7.93 -9.36). This suggests more a misfunctioning of the pH meter than an unusual behaviour of the test solutions. The test results were not affected.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 79
- Sampling time:
- 28 d
- Remarks on result:
- other: Biodegradation starts on day 3 and reaches 61% at the end of the 10-day window (days 3 to 13).
- Details on results:
- The test substance undergoes 79% biodegradation after 28 days in the test conditions. Biodegradation starts on day 3 and reaches 61% at the end of the 10-day window (days 3 to 13), just meeting the 10-day window. Thus the test substance should be regarded as readily biodegradable according to this test.
- Results with reference substance:
- Degradation of sodium benzoate exceeds 40% after 7 days and 65% after 14 days, the activity of the inoculum is thus verified and the test is considered as valid.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The substance is biodegraded by 79% after 28 days in the manometric respirometry test (OECD TG 301F). Biodegradation starts on day 3 and reaches 61% at the end of the 10-day window (days 3 to 13), just meeting the 10-day window criteria. Thus the substance should be classified as readily biodegradable according to this test.
- Executive summary:
Ready biodegradability of the test substance is tested in the Manometric Respirometry Test, according to OECD 301F and in accordance with GLP. Fresh activated sludge form a biological waste water treatment plant treating predominantly domestic sewage (City of Geneva, Aïre) was used. Test substance was tested at 100 mg/L in 250 mL test flasks of the SAPROMAT respirometer, filled with 250 mL of mineral medium. A volume of suspended sludge is added, 2.28 mL sludge (dry weight of suspended solids is 3.289 g/L) is needed, corresponding to a concentration of 30 mg/L (dry weight). About 2 g of soda lime is placed in an attachment of the stopper, the flasks are closed and placed in the water bath. The test substance undergoes 79% biodegradation after 28 days in the test conditions. Biodegradation starts on day 3 and reaches 61% at the end of the 10-day window, just meeting the 10-day window criteria. Thus the test substance should be classified as readily biodegradable according to this test. Degradation of reference substance, Sodium Benzoate, exceeds 40% after 7 days and 65% after 14 days: the activity of the inoculum is thus verified and the test is considered as valid. No toxic effect of the test substance to the micro-organisms was observed at test concentration.
Reference
Biological Oxygen Demand (BOD, mg O2/L, adjusted to nominal concentrations):
|
|
Days: |
3 |
7 |
13 |
14 |
21 |
28 |
BOD Sludge |
1rst flask |
B1 |
6.0 |
16.0 |
16.0 |
17.0 |
19.0 |
21.0 |
|
2nd flask |
B2 |
6.0 |
16.0 |
16.0 |
18.0 |
21.0 |
21.0 |
|
mean |
B |
6.0 |
16.0 |
16.0 |
17.5 |
20.0 |
21.0 |
BOD Test substance |
1rst flask |
C1 |
63.2 |
129.4 |
180.8 |
182.8 |
207.7 |
240.1 |
|
2nd flask |
C2 |
59.9 |
116.2 |
168.2 |
174.1 |
189.6 |
214.6 |
|
1rst fl. Corr. |
C1-B |
57.2 |
113.4 |
164.8 |
165.3 |
187.7 |
219.1 |
|
2nd fl. Corr. |
C2-B |
53.9 |
100.2 |
152.2 |
156.6 |
169.6 |
193.6 |
% Degr. |
1rst flask |
D1 |
22 |
43 |
63 |
63 |
72 |
84 |
|
2nd flask |
D2 |
21 |
38 |
58 |
60 |
65 |
74 |
|
mean |
D |
21 |
41 |
61 |
62 |
68 |
79 |
The curves obtained with the reference substance alone and with the test substance + reference substance show no toxic effect of the test substance to the micro-organisms at the test concentration.
Description of key information
Ready biodegradability of the test substance is tested in the Manometric Respirometry Test, according to OECD 301F and in accordance with GLP. Fresh activated sludge form a biological waste water treatment plant treating predominantly domestic sewage (City of Geneva, Aïre) was used. Test substance was tested at 100 mg/L in 250 mL test flasks of the SAPROMAT respirometer, filled with 250 mL of mineral medium. A volume of suspended sludge is added, 2.28 mL sludge (dry weight of suspended solids is 3.289 g/L) is needed, corresponding to a concentration of 30 mg/L (dry weight). About 2 g of soda lime is placed in an attachment of the stopper, the flasks are closed and placed in the water bath. The test substance undergoes 79% biodegradation after 28 days in the test conditions. Biodegradation starts on day 3 and reaches 61% at the end of the 10-day window, just meeting the 10-day window criteria. Thus the test substance should be classified as readily biodegradable according to this test. Degradation of reference substance, Sodium Benzoate, exceeds 40% after 7 days and 65% after 14 days: the activity of the inoculum is thus verified and the test is considered as valid. No toxic effect of the test substance to the micro-organisms was observed at test concentration.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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