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Diss Factsheets
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EC number: 209-141-4 | CAS number: 556-82-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Basic toxicokinetics
Administrative data
- Endpoint:
- basic toxicokinetics
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented publication.
Data source
Reference
- Reference Type:
- publication
- Title:
- The toxic and metabolic effects of 23 aliphatic alcohols in the isolated perfused rat liver
- Author:
- Strubelt O, Deters M, Siegers C-P and Younes M
- Year:
- 1 999
- Bibliographic source:
- J. Toxicological Sci. 49: 133-142
Materials and methods
- Objective of study:
- metabolism
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The objective of this study was to evaluate the relationship between chemical structure and hepatic efficiacy of the test materials. The toxic, functional, and metabolic effects of several test materials in the perfused, isolated rat liver were investigated.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 3-methylbut-2-en-1-ol
- EC Number:
- 209-141-4
- EC Name:
- 3-methylbut-2-en-1-ol
- Cas Number:
- 556-82-1
- Molecular formula:
- C5H10O
- IUPAC Name:
- 3-methylbut-2-en-1-ol
- Details on test material:
- - Name of test substance: 3-Methyl-2-buten-1-ol
- Source: Merck-Schuchardt
- Analytical purity: analytical grade, no further data on purity
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Weight at study initiation: 320-380 g
- Diet: Altromin pellets standard diet, ad libitum
- Water: tap water, ad libitum
Administration / exposure
- Route of administration:
- other: via the perfusate
- Vehicle:
- not specified
- Details on exposure:
- Liver perfusion:
Removal of the liver and its connection to a recirculation perfusion system was performed. Livers were weighed before they were connected to the perfusion system. The albumin- and serum-free perfusion medium consisted of 250 ml Krebs-Henseleit buffer, ph 7.4; additionally sodium laurocholate (36.7 g/l) was infused into the perfusate at a rate of 1.2 ml/h to stimulate bile secretion. The perfusion medium was continuously gased with carbogen yielding an oxygen partial pressure of 600 mm Hg and kept at 34 °C.
Perfusion was performed under conditions of constant pressure (240 mm H2O) throughout the experiment. The perfusion flow rate was 60 ml/min and the experiments were started after a 30 min equilibration period, adding the test material to the perfusate (time 0) and finished 120 min after initiation. The oxygen consumption was calculated from the difference in oxygen concentrations of the influent and the efluent perfusate using a Micro pH/Blood Gas Analyzer 1306 (Instrumentation Laboratory). The perfusion flow rate was determined every 30 min, bile was sampled every 30 min and the rate of bile secretion was calculated per gram liver and minute.
For biochemical determinations, samples of 2 ml were also taken from the perfusate every 30 min. After the experiments the livers were frozen in liquid nitrogen for further analysis. - Duration and frequency of treatment / exposure:
- 120 min
Doses / concentrations
- Remarks:
- Doses / Concentrations:
65.1 mmol/l
- No. of animals per sex per dose / concentration:
- not applicable
- Control animals:
- other: control experiments were performed in the same way, adding 0.9% saline instead of the test material to the perfusate.
- Details on study design:
- Biochemical determinations:
The activities of glutamate-pyruvate-transaminase (GPT), lactate dehydrogenase (LDH) and glutamate dehydrogenase (GLDH), as well as the concentrations of lactate and pyruvate in the perfusate were assayed using commercial kits from Boehringer, Mannheim. Malondialdehyde (MDA) was measured in the perfusate and in the livers by coupling to thiobarbituric acid. Total glutathione was determined in liver and perfusate samples, oxidized glutathione (GSSG) was estimated after blocking reduced glutathione (GSH) with 2-vinylpyridine. For ATP determination, hepatic tissue was frozen immediately in liquid nitrogen and exracts were prepared. ATP was assayed enzymatically using a reagent kit from Sigma (Munich). - Statistics:
- Means +/- SEM were calculated using the usual manner. The difference between 2 means was calculated using Dunnet´s t-test setting p = 0.05 as the limit of significance. Correlation coefficients were calculated using the program Sigma Plot 3.0 (Jandel Scientific). Their significance was evaluated according to tables published in Documenta Geigy (1969).
Results and discussion
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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