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EC number: 257-827-7 | CAS number: 52301-70-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1973
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 973
- Report date:
- 1973
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: test technique as described in the "Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics" (1959) of the US Association of Food and Drug Officials (AFDO).
- Deviations:
- not specified
- Principles of method if other than guideline:
- None
- GLP compliance:
- no
Test material
- Reference substance name:
- Hexasodium 2,2'-[vinylenebis[(3-sulphonato-4,1-phenylene)imino[6-morpholino-1,3,5-triazine-4,2-diyl]imino]]bis(benzene-1,4-disulphonate)
- EC Number:
- 257-827-7
- EC Name:
- Hexasodium 2,2'-[vinylenebis[(3-sulphonato-4,1-phenylene)imino[6-morpholino-1,3,5-triazine-4,2-diyl]imino]]bis(benzene-1,4-disulphonate)
- Cas Number:
- 52301-70-9
- Molecular formula:
- C40H40N12O20S6.6Na
- IUPAC Name:
- hexasodium 2,2'-[vinylenebis[(3-sulphonato-4,1-phenylene)imino[6-morpholino-1,3,5-triazine-4,2-diyl]imino]]bis(benzene-1,4-disulphonate)
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- other: Russian breed
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Housing: single metal cages
- Diet (e.g. ad libitum): Nafag ad libitum
- Water (e.g. ad libitum): Drinking water ad libitum
- Acclimation period: 8 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18+/-1 °C
- Humidity (%): 55+/-5 %
- Photoperiod (hrs dark / hrs light): 14 hours light/day
Test system
- Type of coverage:
- occlusive
- Preparation of test site:
- shaved
- Vehicle:
- not specified
- Controls:
- no
- Amount / concentration applied:
- The substance was applied to each side in quantities of 0.5 ml.
- Duration of treatment / exposure:
- 24 h
- Observation period:
- The gauze patches were removed 24 hours after application. The reaction of the skin was appraised upon removal during an observation period of 9 days.
- Number of animals:
- 6 (3 Males and 3 Females)
- Details on study design:
- 6 rabbits ( 3 males; 3 females) of the Russian breed, weighing 1.4 to 1.7 kg, were shaved one day before treatment with an electric clipper on the flanks. The shaved skin area on the left side was slightly scarified just before treatment. A gauze patch of 2.5 x 2.5 cm laden with test substance was applied immediately to the prepared skin. The patch was covered with an impermeable foil of 5x5 cm, which was fixed to the body of the animals with adhesive tape. The substance was applied to each side in quantities of 0.5 ml . The gauze patches were removed 24 hours after application. The reaction of the skin was appraised upon removal during an observation period of 9 days.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0.1
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks on result:
- other: with intact skin
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- fully reversible
- Remarks on result:
- other: with intact skin
- Irritant / corrosive response data:
- None
- Other effects:
- None
Any other information on results incl. tables
Scores for erythema
Erythema with intact skin | ||||
Animal | Observation time | |||
24 hours | 48 hours | 72 hours | ||
1 | 0 | 0 | 0 | |
2 | 0 | 0 | 0 | |
3 | 0 | 0 | 0 | |
4 | 1 | 0 | 0 | |
5 | 0 | 0 | 0 | |
6 | 1 | 0 | 0 | |
Mean | 0.33 | 0 | 0 |
Erythema with abraded skin | ||||
Animal | Observation time | |||
24 hours | 48 hours | 72 hours | ||
1 | 1 | 1 | 0 | |
2 | 1 | 1 | 0 | |
3 | 1 | 0 | 0 | |
4 | 1 | 0 | 0 | |
5 | 1 | 1 | 1 | |
6 | 1 | 0 | 0 | |
Mean | 1.00 | 0.50 | 0.17 |
Scores for oedema
Oedema with intact skin |
||||
Animal | Observation time | |||
24 hours | 48 hours | 72 hours | ||
1 | 0 | 0 | 0 | |
2 | 0 | 0 | 0 | |
3 | 0 | 0 | 0 | |
4 | 0 | 0 | 0 | |
5 | 0 | 0 | 0 | |
6 | 0 | 0 | 0 | |
Mean | 0 | 0 | 0 |
Oedema with abraded skin | ||||
Animal | Observation time | |||
24 hours | 48 hours | 72 hours | ||
1 | 0 | 0 | 0 | |
2 | 0 | 0 | 0 | |
3 | 0 | 0 | 0 | |
4 | 0 | 0 | 0 | |
5 | 0 | 0 | 0 | |
6 | 0 | 0 | 0 | |
Mean | 0 | 0 | 0 |
Applicant's summary and conclusion
- Interpretation of results:
- other: Not classified as irritant according to the CLP Regulation (EC) No.1272/2008
- Conclusions:
- The substance was found to cause a slight erythema without oedema with the intact skin. Hence, it does not warrant classification as per the Regulation EC No. 1272/2008.
- Executive summary:
The skin irritation potential of the test item was evalauted in a study conducted according to the patch test technique as described in the "Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics" (1959) of the US Association of Food and Drug Officials (AFDO). The test item was applied to each side of the shaved flank region of 6 Russian breed rabbits in quantities of 0.5 ml . The shaved skin area on the left side was slightly scarified just before treatment. A gauze patch of 2.5 x 2.5 cm laden with test substance was applied immediatly to the prepared skin. The patch was covered with an impermeable foil of 5x5 cm, which was fixed to the body of the animals with adhesive tape. The gauze patches were removed 24 hours after application. The reaction of the skin was appraised upon removal during an observation period of 9 days. A slight erythema without oedema was observed with the intact skin. Hence, the test item does not warrant classification as per the Regulation EC No. 1272/2008.
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