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EC number: 944-449-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 04 Mar - 01 Apr 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- adopted 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Staatliches Gewerbeaufsichtsamt Hildesheim
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: supernatant from the centrifuged activated sludge of a local municipal STP
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant, D-31137 Hildesheim
- Storage conditions: by aeration with CO2 free air
- Storage length: 4 days
- Preparation of inoculum for exposure: The activated sludge was washed twice with autoclaved tap water. After the second washing the settled sludge was filled up with mineral salts medium and was maintained in an aerobic condition by aeration for 4 hours. Thereafter the sludge was homogenized with a blender. The supernatant was decanted and maintained in an aerobic condition by aeration with CO2 free air for 4 days.
- Concentration of sludge: 1.7 x 10^9 cfu/L (corresponding to cfu concentration of the inoculum in the final test solution)
- Initial cell/biomass concentration: 0.2 mL inoculum per test vessel was used to initiate inoculation. The incubation bottles were closed without air bubbles. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 3 mg/L
- Based on:
- ThOD
- Remarks:
- 2.57 mg O2/mg
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline (Mineral salts medium according to guideline)
- Test temperature: 20 - 21 °C
- pH: 7.37
- Aeration of dilution water: yes. One day before test start the demineralised water for the test medium was aerated until oxygen saturation and was then left standing at room temperature for at least 20 h
- Continuous darkness: yes
- Other: Detailes of parameters of the test media is listed in Table 1 in the section "Any other information on material and methods incl. tables"
TEST SYSTEM
- Culturing apparatus: lncubator (RUBARTH), BOD bottles 300 mL with glass stoppers
- Number of culture flasks/concentration: 5 x 2 per test item, reference item, toxicity control and control
- Method used to create anaerobic conditions: the activated sludge was maintained in an aerobic condition by aeration for 4 hours
- Measuring equipment: Oximeter, HQ 40d multi, HACH
- Test performed in closed vessels due to significant volatility of test substance: yes
SAMPLING
- Sampling frequency: 0, 7, 14, 21 and 28 days
- Sampling method: oxygen concentration was measured
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 2 bottles per sampling
- Toxicity control: yes, 2 bottles per sampling
- Other: Reference control: yes, 2 bottles per sampling
STATISTICAL METHODS: no statistics - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 80
- Sampling time:
- 28 d
- Details on results:
- The test item reached the 10% level (end of lag-phase) after 3 days. The pass level of a biodegradation of > 60% was reached after 12 days. The biodegradation came to a maximum of 87% after 21 days. The test item is classified as readily biodegradable within the 10-d-window.
The biodegradation rate of the toxicity control came to 87% after 14 days. The biodegradation of the reference item was not inhibited by the test item. The reference control reached the pass level > 60% after 7 days.
Results in details are listed in Table 1 and 2 within the section "any other information on results incl. tables". - Results with reference substance:
- The reference control reached the pass level > 60% after 7 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Readily biodegradable (80% in 28 days, 10 day window passed); Closed Bottle Test; Toxicity control: Test substance not inhibitory at tested concentration of 1.5 mg/L
Reference
Table 1: Biodegradation of the Test ltem in Comparison to the Reference Control and the Toxicity Control
|
biodegradation [%]
study day [d] |
|||
|
7 |
14 |
21 |
28 |
Test Item 3.0 mg/L |
25 |
75 |
87 |
80 |
Reference control 10 mg/L |
74 |
86 |
86 |
78 |
Toxicity Control 1.5 mg/L Test ltem + 2.5 mg/L Reference Item |
50 |
87 |
89 |
88 |
Table 2: Oxygen Determination of the lnoculum Control and Functional Control
Study day |
Inoculum Control
|
Functional Control, 5 mg/L
ThOD: 1.67 mg 02/mg |
||||||||
O2 [mg/L] |
O2 [mg/L] |
BOD |
Degr. |
|||||||
[d] |
C1 |
C2 |
mv |
O2-depl. |
R1 |
R2 |
mv |
[mg O2/L] |
[mg O2/mg] |
[%] |
0 |
9.07 |
9.13 |
9.10 |
- |
9.08 |
9.08 |
9.08 |
- |
- |
- |
7 |
8.10 |
8.03 |
8.07 |
1.04 |
2.05 |
1.61 |
1.83 |
6.22 |
1.24 |
74 |
14 |
8.54 |
7.73 |
8.14 |
0.97 |
0.96 |
(6.49) |
0.96 |
7.16 |
1.43 |
86 |
21 |
8.84 |
7.51 |
8.18 |
0.92 |
1.31 |
0.70 |
1.01 |
7.15 |
1.43 |
86 |
28 |
7.42 |
7.55 |
7.49 |
1.62 |
(8.61) |
0.97 |
0.97 |
6.50 |
1.30 |
78 |
Table 3: Oxygen Determination of the Test ltem and the Toxicity Control
study day
|
Test Item, 3.0 mg/L |
Taxicity Control 1.5 mg/L test item + 2.5 mg/L reference item |
||||||||||
ThOD: 2.57 mg O2/mg |
ThOD: 2.03 mg O2/mg |
|||||||||||
[d] |
O2 [mg/L] |
BOD |
Degr. |
O2 [mg/L] |
BOD |
Degr. |
||||||
|
P1 |
P2 |
mv |
[mgO2/L] |
[mgO2/mg] |
[%] |
T1 |
T2 |
mv |
[mg O2/L] |
[mg O2/mg] |
[%] |
0 |
9.00 |
9.05 |
9.03 |
- |
- |
- |
9.06 |
9.05 |
9.06 |
- |
- |
- |
7 |
6.15 |
6.02 |
6.09 |
1.91 |
0.64 |
25 |
4.00 |
3.91 |
3.96 |
4.07 |
1.02 |
50 |
14 |
2.55 |
1.94 |
2.25 |
5.82 |
1.94 |
75 |
0.71 |
1.38 |
1.05 |
7.05 |
1.76 |
87 |
21 |
1.58 |
1.15 |
1.37 |
6.74 |
2.25 |
87 |
1.37 |
0.47 |
0.92 |
7.21 |
1.80 |
89 |
28 |
1.31 |
1.13 |
1.22 |
6.19 |
2.06 |
80 |
0.28 |
(8.41) |
0.28 |
7.16 |
1.79 |
88 |
mv degr. depl.
mv= mean value
dgra.= degradation
depl.= depletion
ThOD = Theoretical oxygen demand
BOD = Biological oxygen demand
( ) = Outlier, not included in calculations
Description of key information
Readily biodegradable (80 % within 28 d, OECD 301 D)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
One GLP guideline study according to OECD 301 D is available investigating the ready biodegradability of the substance. A mixed microbial population of the supernatant from the centrifuged activated sludge of a local municipal STP was inoculated with a nominal concentration of 3.0 mg/L test substance and incubated for 28 d under controlled conditions in the dark. Degradation was followed by the determination of dissolved oxygen content after 0 h, 7, 14, 21 and 28 d. An inoculum blank, a procedure control (with 10.0 mg reference substance/L) and a toxicity control were run in parallel. The derived 28 d degradation rate was 80 % for the test substance and 88 % for the toxicity control. Since the test substance reached the pass level for ready biodegradability of > 60% removal of ThOD within 28 d (meeting the 10 d – window) it could be classified as readily biodegradable. Due to the fact that the degradation rate of the toxicity control was > 25% after 14 d, toxic effects of the test substance to the inoculum can be excluded.
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