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EC number: 208-096-8 | CAS number: 509-34-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from J-check database
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 (Bioconcentration: Flow-through Fish Test)
- Principles of method if other than guideline:
- Bioaccumulation test was conducted for 28 days for estimating the bioconcentration factor (BCF) and lipid content of test substance 3',6'Bis(diethylamino)spiro[isobenzofuran1(
3H),9'[9H]xanthene]3one in test organism Cyprinus carpio. - GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): C.I. Solvent Red 49
- Molecular formula (if other than submission substance): C28H30N2O3
- Molecular weight (if other than submission substance): 442.556 g/mol
- Smiles notation (if other than submission substance): C12(c3c(Oc4c1ccc(c4)N(CC)CC)cc(N(CC)CC)cc3)c1c(cccc1)C(O2)=O
- InChI: 1S/C28H30N2O3/c1-5-29(6-2)19-13-15-23-25(17-19)32-26-18-20(30(7-3)8-4)14-16-24(26)28(23)22-12-10-9-11-21(22)27(31)33-28/h9-18H,5-8H2,1-4H3
- Substance type: Organic
- Physical state: Solid
- Purity:99.6 % (HPLC and LCMS)
- Water solubility : 851 mg/L (25 degreeC) - Radiolabelling:
- not specified
- Details on sampling:
- No data available
- Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
-Name and concentration of vehicle :
1st Concentration area : HCO40 50 microg/L, N,NDimethylformamide 50000 microg/L,
2nd Concentration area : HCO40 5 microg/L, N,NDimethylformamide 50000 microg/L - Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- No data available
- Route of exposure:
- aqueous
- Test type:
- flow-through
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 28 d
- Hardness:
- No data available
- Test temperature:
- No data available
- pH:
- No data available
- Dissolved oxygen:
- No data available
- TOC:
- No data available
- Salinity:
- No data available
- Conductivity:
- No data available
- Details on test conditions:
- RANGE FINDING / PRELIMINARY STUDY
-Test concentrations : LC50(96h) > 10.0 mg/L, Rice fish(Oryzias latipes) - Nominal and measured concentrations:
- Nominal concentrations-
1st concentration area: 10 mg/l
2nd concentration area: 1 mg/l - Reference substance (positive control):
- not specified
- Details on estimation of bioconcentration:
- No data available
- Lipid content:
- 2.87 %
- Time point:
- start of exposure
- Lipid content:
- 1.94 %
- Time point:
- end of exposure
- Key result
- Conc. / dose:
- 10 µg/L
- Type:
- BCF
- Value:
- <= 0.91 L/kg
- Basis:
- other: Test material
- Time of plateau:
- 28 d
- Calculation basis:
- other: No data
- Key result
- Conc. / dose:
- 1 µg/L
- Type:
- BCF
- Value:
- <= 9.1 L/kg
- Basis:
- other: test material
- Time of plateau:
- 28 d
- Calculation basis:
- other: No data
- Details on kinetic parameters:
- No data available
- Metabolites:
- No data available
- Results with reference substance (positive control):
- No data available
- Details on results:
- Recovery ratio :
Test water : 97.8 %,
Fish : 91.9 %, - Reported statistics:
- No data available
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The bioconcentration factor (BCF) for test substance 3',6'Bis(diethylamino)spiro[isobenzofuran1(3H),9'[9H]xanthene]3one (Cas no.509-34-2) was determined according to OECD Guideline 305 (Bioconcentration: Flow through Fish Test). The BCF value was observed to be <= 0.91 L/kg at dose concentration 10 microg/L and <= 9.1 L/kg at dose concentration 1 microg/L on test organism Cyprinus carpio during 28 day period.
- Executive summary:
Bioaccumulation test was conducted for 28 days for determination the bioconcentration factor (BCF) and lipid content of test substance 3',6'Bis(diethylamino)spiro[isobenzofuran1(3H),9'[9H]xanthene]3one (Cas no.509-34-2) on test organism Cyprinus carpio.
The purity of test substance by HPLC and LCMS was found to be 99.6% and water solubility value is 851mg/l at 25ᵒC .The recovery ratio is given-Test water: 97.8 % and Fish: 91.9 %
And Nominal concentrations used in the study as - 1st concentration area: 10 microg/L, 2nd concentration area: 1 microg/L and Range finding study was carried out on Rice fish (Oryzias latipes) and the 96 hr LC50 value was found to be > 10.0 mg/L.
By analytical methods, the limit of quantification was found to be-
Test water: (Fish: 9.3 ng/g)
1st concentration area: 0.18 µg/l
2nd concentration area: 0.018µg/l
Whereas the lipid content at the start and end of exposure was found to be 2.87% and 1.94%, respectively.
Thus according to guideline OECD Guideline 305 (Bioconcentration: Flow through Fish Test) the BCF value for test substance 3',6'Bis(diethylamino)spiro[isobenzofuran1(3H),9'[9H]xanthene]3one (Cas no.509-34-2) was observed to be <= 0.91 L/kg at dose concentration 10 microg/L and <= 9.1 L/kg at dose concentration 1 microg/L on test organism Cyprinus carpio during 28 day period.
These values indicate that the substance is not bioaccumulative.
Reference
Bioconcentration test results:
|
|
9 d |
13 d |
15 d |
20 d |
28 d |
1st concentration area |
Test substance concentration (microg/L) |
10.4 |
10.5 |
9.86 |
9.83 |
10.1 |
Bioconcentration factor (L/kg) |
<= 0.91 |
<= 0.91 |
<= 0.91 |
<= 0.91 |
<= 0.91 |
|
<= 0.91 |
<= 0.91 |
<= 0.91 |
<= 0.91 |
<= 0.91 |
||
2nd concentration area |
Test substance concentration (microg/L) |
1.06 |
1.04 |
1.00 |
1.01 |
0.998 |
Bioconcentration factor (L/kg) |
<= 9.1 |
<= 9.1 |
<= 9.1 |
<= 9.1 |
<= 9.1 |
|
<= 9.1 |
<= 9.1 |
<= 9.1 |
<= 9.1 |
<= 9.1 |
Description of key information
The bioconcentration factor (BCF) for test substance 3',6'Bis(diethylamino)spiro[isobenzofuran1(3H),9'[9H]xanthene]3one (Cas no.509-34-2) was determined according to OECD Guideline 305 (Bioconcentration: Flow through Fish Test). The BCF value was observed to be <= 0.91 L/kg at dose concentration 10 microg/L and <= 9.1 L/kg at dose concentration 1 microg/L on test organism Cyprinus carpio during 28 day period.
Key value for chemical safety assessment
- BCF (aquatic species):
- 0.91 L/kg ww
Additional information
Experimental data of Bioaccumulation endpoint for the target chemical C.I. Solvent Red 49 (Cas no. 509-34-2) and its read across chemical which is 70 -80 % structurally similar to target chemical were reviewed to summarize as follows:
Study 1:
Authorative database Japan chemicals collborative knowledge database (J-check);2017 for target chemical C.I. Solvent Red 49 (Cas no. 509-34-2)indicate Bioaccumulation test was conducted for 28 days for determination the bioconcentration factor (BCF) and lipid content of test substance 3',6'Bis(diethylamino)spiro[isobenzofuran1(3H),9'[9H]xanthene]3one (Cas no.509-34-2) on test organism Cyprinus carpio. The purity of test substance by HPLC and LCMS was found to be 99.6% and water solubility value is 851mg/l at 25ᵒC .The recovery ratio is given-Test water: 97.8 % and Fish: 91.9 % And Nominal concentrations used in the study as- 1stconcentration area: 10 microg/L, 2ndconcentration area: 1 microg/L and Range finding study was carried out on Rice fish (Oryzias latipes) and the 96 hr LC50 value was found to be > 10.0 mg/L.
By analytical methods, the limit of quantification was found to be-
Test water: (Fish: 9.3 ng/g)
1st concentration area: 0.18 µg/l
2nd concentration area: 0.018µg/l
The lipid content at the start and end of exposure was found to be 2.87% and 1.94%, respectively. Thus according to guideline OECD Guideline 305 (Bioconcentration: Flow through Fish Test) the BCF value for test substance 3',6'Bis(diethylamino)spiro[isobenzofuran1(3H),9'[9H]xanthene]3one (Cas no.509-34-2) was observed to be <= 0.91 L/kg at dose concentration 10 microg/L and <= 9.1 L/kg at dose concentration 1 microg/L on test organism Cyprinus carpio during 28 day period. Reported bioaccumulation factor i.e BCF value suggested that the test substance C.I. Solvent Red 49 was non bioaccumulative in nature as it does not exceed the BCF criteria of 2000.
Study 2:
From same database i.e Japan chemicals collborative knowledge database (J-check) (2017); data for read across chemical Fluorescein sodium (Cas no. 518-47-8) indicate Bioaccumulation test was conducted for 28 days for estimating the bioconcentration factor (BCF) and lipid content of test substance Fluorescein sodium in test organism Cyprinus carpio. The purity of test substance by HPLC was found to be 92.1% and water solubility value is≥300 g/l at 20ᵒC (Flask method). The recovery ratio is given-Test water: 100% (The test solution were input into analytical equipment directly.)Fish: 93.8%
Nominal concentrations used in the study are given-1stconcentration area: 0.46 mg/l; 2ndconcentration area: 0.046 mg/l.Range finding study was carried out on Rice fish (Oryzias latipes) and the 96 hr LC50 value was found to be >200mg/l. By analytical methods, the limit of quantification was found to be-Test water: 1stconcentration area: 11 µg/l and 2ndconcentration area: 1.1 µg/l; Fish: 120 ng/g.The lipid content at the start and end of exposure was found to be 2.94% and 4.14%, respectively. The test substance concentration in environment during a period of 28 days was 0.46 mg/l, 0.046 mg/l and bioconcentration (BCF) value of test substance was found to be≤0.27 l/kg, respectively. Thus, the test substance has a low potential of bioaccumulation in test organism Cyprinus carpio during 28 day period.
Study 3:
In third study Information from CHRIP National Institute of Technology and Evaluation , 2017 database for read across Rhodamine B (Cas no.81-88-9) indicate Bioaccumulation study was conducted in cyprinus carpio for 6 weeks and the BCF was determined to be 0.2 and 1.7 L/Kg at the concentrations of 100 and 10 microgram /l.
Study 4:
Data from HSDB database; 2017 for read across chemical fluorescein (Cas no. 2321-07-5) indicate an estimated BCF value of 75 was calculated for fluorescein, using an estimated log Kow of 3.35 and a recommended regression-derived equation. This BCF value suggests that bioconcentration of fluorescein in aquatic organisms will not be an important fate process as it is non bioaccumulative in nature.
Study 2, 3 and 4 of read across study supported to target study of 1.
Thus based on the above all brief summaries for target and read across which are structurally similar (70 -80%) it is concluded that the test substance C.I. Solvent Red 49 (Cas no. 509-34-2) is non Bioaccumulative in aquatic environment.
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