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EC number: 201-283-5 | CAS number: 80-48-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / micronucleus study
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2004
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 487 (In vitro Mammalian Cell Micronucleus Test)
- Version / remarks:
- Micronucleus test in mouse lymphoma
(L5178Y) cells - Deviations:
- not specified
- Principles of method if other than guideline:
- Mouse lymphoma cells were obtained from Hofmann-La Roche (Basel, Switzerland). Based on the data of a preliminary solubility test and within the limit to test up to 10 mM or 5 mg/ml (whichever was lower), 10 concentrations were selected for the main experiment.
All compounds were dissolved in DMSO. The cells were diluted in RPMI-10 medium (Gibco) to 200,000 cells/ml for the 3 h treatment and to 100,000 cells/ml for the 20 h treatment (cell count determined by use of a SYSMEX cell counter), respectively. The desired final concentration was obtained by adding 10 microlitres of DMSO-dissolved test compound to 1 ml cell suspension (sufficient for four wells of 200 microlitre each), resulting in 1% DMSO in the treatment medium. Incubation was conducted with 96-well plates with four replicates per test compound concentration.
For the micronucleus experiment, the cells were treated with the test compound solution for 3 h (with or without S9) or for 20 h (without S9 only). After the treatment, cells were washed with phosphate-buffered saline and further incubated with fresh medium for 21 h in case of 3 h treatment, or 28 h in case of 20 h treatment. Before sampling, a cell count for one of the replicates of each concentration was determined as cytotoxicity parameter, and the concurrent negative control was set to 100%. Based on the relative cell count, the concentration that inhibited growth by approximately 50–70%, and two lower concentrations showing less cytotoxicity were chosen for analysis. The selected cell suspensions were then spread on glass slides by cytocentrifugation (Shandon Cytospin). Cells were fixed and stained with Schiff’s reagent (nucleus staining) and Congo-Red counterstaining for the cytoplasm. Two thousand cells (1000 cells per culture) were analyzed from at least three concentrations of each test compound, as well as from negative and positive controls. - GLP compliance:
- not specified
- Type of assay:
- in vitro mammalian cell micronucleus test
Test material
- Reference substance name:
- Methyl toluene-4-sulphonate
- EC Number:
- 201-283-5
- EC Name:
- Methyl toluene-4-sulphonate
- Cas Number:
- 80-48-8
- Molecular formula:
- C8H10O3S
- IUPAC Name:
- methyl 4-methylbenzene-1-sulfonate
Constituent 1
- Specific details on test material used for the study:
- Source: Fluka, Switzerland, no. 89800
Method
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Additional strain / cell type characteristics:
- not specified
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Vehicle / solvent:
- DMSO
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- no
- Details on test system and experimental conditions:
- The historical average solvent control data for micronucleus induction were as follows: 3 h treatment without S9, 0.73 ± 0.13; 20 h treatment without S9, 0.82 ± 0.18; 3 h treatment with S9, 0.78 ± 0.16.
- Evaluation criteria:
- A result was considered positive when
the micronucleus frequency was at least twice the historical negative control value as well as twice the actual concurrent negative solvent control value.
These criteria are based on an in-house validation study of the assay.
Results and discussion
Test results
- Key result
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity, but tested up to precipitating concentrations
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Remarks on result:
- mutagenic potential (based on QSAR/QSPR prediction)
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.