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Diss Factsheets
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EC number: 212-889-4 | CAS number: 877-24-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: Refer below principle
- Principles of method if other than guideline:
- To study the toxicity of Phthalates to the Marine Dinoflagellate Gymnodinium breve
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- - Name of test material: 1,2-Benzenedicarboxylic acid, monopotassium salt
- Molecular formula: C8 H6 O4.K
- Molecular weight: 204.221 g/mol
- Smiles notation: c1(c(cccc1)C(=O)[O-])C(O)=O.[K+]
- InChl: 1S/C8H6O4.K/c9-7(10)5-3-1-2-4-6(5)8(11)12;/h1-4H,(H,9,10)(H,11,12);/q;+1/p-1-
Substance type: Organic
- Physical state: Solid - Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: 0,1,10, 20, 50, 100, 200, 500, 1000 and 1000 ppm
- Sampling method: Potassium hydrogen phthalate is prepared by 1% solutions of these compounds in distilled water, i.e. 1.0 g of the compound in I00 ml of distilled water.
- Sample storage conditions before analysis: Test portions were placed in a single row in test tube racks which were placed 2 inches from 40-watt, cool-white fluorescent lights (incident light ca. I000 foot-candles) in a 25˚ constant room temperature. Special care was taken to insure uniform light on test portions to avoid variability of relative chlorophyll a values. - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Potassium hydrogen phthalate is prepared by 1% solutions of these compounds in distilled water, i.e. 1.0 g of the compound in I00 ml of distilled water.
- Controls: Yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): distilled water
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 0.1, 0.2, 0.5, 1.0 ml in distilled water. - Test organisms (species):
- other: Dinoflagellate Gymnodinium breve
- Details on inoculum:
- - Laboratory culture: Yes
- Method of cultivation: Gymnodinium breve that were grown in an artificial sea water medium--NH-15 were used and were transferred 14 days prior to use so that they would be in an active logarithmic growth phase.
- Initial biomass concentration: 1000 and 5000 G. breve per milliliter. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- No data
- Test temperature:
- 25˚C
- pH:
- 7.6
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Conductivity:
- No data
- Nominal and measured concentrations:
- No data
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Artificial sea water medium--NH-15.
- No. of organisms per vessel: 1000 and 5000 G. breve per milliliter.
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 2
- Biomass loading rate: The cultures contained between 1000 and 5000 G. breve per milliliter at the time the assays were started.
OTHER TEST CONDITIONS
- Adjustment of pH: Yes, pH 4.2 adjusted to pH 7.6
- Photoperiod: Constant
- Light intensity: cool-white fluorescent lights (incident light ca. I000 foot-candles)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Relative chlorophyll a values were observed. - Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 498.52 - 997 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The pH of these media (4.2) to be responsible, thus an additional assay was carried out in which the pH of the one percent solution of potassium hydrogen phthalate was adjusted to 7.6 with potassium hydroxide (yielding the dipotassium salt in situ) before adding aliquots to the culture portions.This adjustment alleviated the observed toxicity.
- Reported statistics and error estimates:
- Values were obtained by plotting on semilog paper the concentration of test material (ppm) versus the growth rate (linear axis of graph), expressed as a percentage of the growth of a "no-add" control. The concentration values (ppm) which caused a 50 percent growth reduction.
- Validity criteria fulfilled:
- no
- Conclusions:
- EC50 for Potassium hydrogen phthalate was found to be 498.52 and 997 mg/l when tested on Marine Dinoflagellate Gymnodinium breve for 96 hours.
- Executive summary:
In a study of toxicity of Phthalates to the Marine Dinoflagellate Gymnodinium breve the effect of Potassium hydrogen phthalate was evaluated. The test substance was tested in a concentration of 0,1,10, 20, 50, 100, 200, 500, 1000 and 1000 ppm. The results show Median growth when concentration of test material (ppm) versus the growth rate (linear axis of graph), expressed as a percentage of the growth of a "no-add" control at 498.52 and 997 mg/l. Therefore, EC50 of Potassium hydrogen phthalate was considered to be be 498.52 and 997 mg/l when tested on Marine Dinoflagellate Gymnodinium breve for 96 hours.
Reference
Description of key information
EC50 for Potassium hydrogen phthalate was found to be 498.52 and 997 mg/l when tested on Marine Dinoflagellate Gymnodinium breve for 96 hours.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 498.52 mg/L
Additional information
In a study of toxicity of Phthalates to the Marine Dinoflagellate Gymnodinium breve the effect of Potassium hydrogen phthalate was evaluated. The test substance was tested in a concentration of 0,1,10, 20, 50, 100, 200, 500, 1000 and 1000 ppm. The results show Median growth when concentration of test material (ppm) versus the growth rate (linear axis of graph), expressed as a percentage of the growth of a "no-add" control at 498.52 and 997 mg/l. Therefore, EC50 of Potassium hydrogen phthalate was considered to be be 498.52 and 997 mg/l when tested on Marine Dinoflagellate Gymnodinium breve for 96 hours.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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