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EC number: 213-138-3 | CAS number: 926-57-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP-Guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 1,3-dichlorobut-2-ene
- EC Number:
- 213-138-3
- EC Name:
- 1,3-dichlorobut-2-ene
- Cas Number:
- 926-57-8
- Molecular formula:
- C4H6Cl2
- IUPAC Name:
- 1,3-dichlorobut-2-ene
- Details on test material:
- - Name of test material (as cited in study report): 1,3-dichlorobut-2-ene
- Physical state: yellow-brown liquid
- Analytical purity: 99.1%
- Stability under test conditions: The analytical studies on the stability of the test substance in medium showed that 1,3-dichlorobut-2-ene in the concentration range 10-200 mg/mL and over the period of use was stable. Stability is therefore assumed also for the slightly deviating concentration of 9 mg/mL in the lowest dose, stability under these conditions.
- Storage condition of test material: at room temperature.
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: WISW (SPF Cpb)
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen, Germany.
- Age at study initiation: about 8 (males) and 10 (females) weeks old according to their weight.
- Weight at study initiation: 193 g (average weight, males), 170 g (average weight, females).
- Fasting period before study: yes, from about 16 hours before until to 4 hours after the treatment, the food was withdrawn.
- Housing: in groups of 5 animals in Makrolon cages Type III.
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 2
- Humidity (%): 50 ± 10
- Air changes (per hr): 10 times per hour
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: 1,2-Propandiol
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 9 mg/mL, 20 mg/mL, 44.8 mg/mL, 100 mg/mL
- Amount of vehicle (if gavage): 10 mL kg bw
MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg - Doses:
- 90, 200, 448, 1000 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- other: not necessary
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: general conditions and clinical signs were observed twice per day (once a day during weekends and public holidays).
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs and body weight. - Statistics:
- LD50 was derived according to Spearman-Kärber.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 414 mg/kg bw
- Based on:
- test mat.
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 300 mg/kg bw
- Based on:
- test mat.
- Mortality:
- MALES
90 mg/kg bw: no fatalities were observed;
200 mg/kg bw: no fatalities were observed;
448 mg/kg bw: 3/5 animals (60%) died during a range period between 4 hours and 1 day;
1000 mg/kg bw: all animals died (100%) within 1 hour from the application.
FEMALES
90 mg/kg bw: no fatalities were observed;
200 mg/kg bw: no fatalities were observed;
448 mg/kg bw: 5/5 animals (100%) died during a range period between 4 hours and 1 day;
1000 mg/kg bw: all animals died (100%) within 1 hour from the application. - Clinical signs:
- The symptoms after dosing of 200 to 1000 mg/kg were ruffled fur and sedation. After 1000 mg/kg all animals showed salivation. At the dose of 90 mg/kg per body weight there were no symptoms in either male or female rats.
- Body weight:
- Three rats in the 200 mg/kg group and one male in the 448 mg/kg dose group had a small weight loss.
- Gross pathology:
- The pathology of animals that died (during the experiment) showed very congested vessels in the stomach as well as very reddened mucous membranes. The small intestine was also much reddened. After the 1000 mg/kg dose, the stomach was filled with a clear fluid, and after the 448 mg/kg dose it was filled with a thin watery food mush. The pathology of the animals at the end of the experiment showed that in males of the 448 mg/kg group the liver was grown together with the stomach and peritoneum. All of the other animals were normal.
Any other information on results incl. tables
Based on the study results the test substance is to be classified as Xn; R22 (Harmful if swallowed) according to DSD-DPD criteria and Acute Tox 3 (H301; Toxic if swallowed) according to CLP criteria.
Applicant's summary and conclusion
- Executive summary:
Bomhard (1991)
In an acute oral toxicity study fasted male and female WISW (SPF Cpb) rats were administered 90, 200, 448, 1000 mg/kg bw 1,3-dichlorobut-2-ene. The animals were observed for 14 days after administration. No mortalities occurred at 90 and 200 mg/kg bw in male and female animals. Mortalities occurred in males at 448 and 1000 mg/kg bw (60 % and 100% mortality respectively) and in females at 448 and 1000 mg/kg bw (100% at both doses). The acute oral LD50 was calculated to be 414 mg/kg bw for male animals and 300 mg/kg bw for female animals. The following signs of clinical toxicity were reported: after dosing of 200 to 1000 mg/kg ruffled fur and sedation. After 1000 mg/kg all animals showed salivation. At the dose of 90 mg/kg per body weight there were no symptoms in either male or female rats. Three rats in the 200 mg/kg group and one male in the 448 mg/kg dose group had a small weight loss. The pathology of animals that died during the experiment showed very congested vessels in the stomach as well as very reddened mucous membranes. The small intestine was also much reddened. After the 1000 mg/kg dose, the stomach was filled with a clear fluid, and after the 448 mg/kg dose it was filled with a thin watery food mush. The pathology of the animals at the end of the experiment showed that in males of the 448 mg/kg group the liver was grown together with the stomach and peritoneum. All other animals were normal.
Based on the study results the test substance is to be classified as Xn; R22 (Harmful if swallowed) according to DSD-DPD criteria and Acute Tox 3 (H301; Toxic if swallowed) according to CLP criteria.
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