Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
Reaction mass of sodium amino-bis{[4-(ethenylsubstituted)phenyl]diazenyl}-hydroxynaphthalenesulfonate and polysodium amino-{[4-(ethenylsubstituted)phenyl]diazenyl}-{[4-(ethenylsubstituted)-2-sulfonatophenyl]diazenyl}-hydroxynaphthalenesulfonate and polysodium amino-bis{[4-(ethenylsubstituted)-2-sulfonatophenyl]diazenyl}-hydroxynaphthalenesulfonate
EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- Guidelines followed
- GLP compliance:
- yes
- Specific details on test material used for the study:
- None
- Analytical monitoring:
- yes
- Details on sampling:
- None
- Vehicle:
- yes
- Details on test solutions:
- None
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Not available
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Each test vessel was checked for immobilised daphnids and any abnormal behavior or appearance at 24 and 48 hours
Daphnids that were not able to swim within 15 seconds, after gentle agitation of the test vessel were considered to be immobilised (even if they can still move their antennae). On completion of test, dead daphnids found and all live daphnids (were euthanized with MS222 medium) were discarded in the drain along with the test solutions - Hardness:
- 213 mg/L
- Test temperature:
- Control : 21.7 - 21.8 °C
Treatment : 22.1 – 23.8 °C - pH:
- Control : 7.5 - 7.8
Treatment : 7.4 - 7.7 - Dissolved oxygen:
- Control : 7.82 – 7.89 mg/L
Treatment : 7.53 – 7.85 mg/L - Salinity:
- Not available
- Nominal and measured concentrations:
- 0 hour – Mean Recovery results: 91.91 %, 93.19 % and 95.75 % for 2.5 mg/L, 26.62 mg/L and 128.84 mg/L concentrations respectively.
48 hour – Mean Recovery results: 90.43 %, 91.92 % and 94.35 % for 2.5 mg/L, 26.62 mg/L and 128.84 mg/L concentrations respectively. - Details on test conditions:
- None
- Reference substance (positive control):
- yes
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 34.58 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- dissolved
- Basis for effect:
- mobility
- Details on results:
- None
- Results with reference substance (positive control):
- Acute immobilisation toxicity study in Daphnia magna, was performed with Potassium Dichromate Pure (Batch No. MG0M602109) in accordance with the guideline OECD 202. Daphnids were exposed to a range of five different test concentrations viz., 0.4, 0.72, 1.30, 2.33 and 4.20 mg/L. Four replicates (5 daphnids in each test replicate) were maintained for each test concentration and control group was maintained along with the test concentrations. The test item was formulated with M4 medium.
The daphnids with brood chambers were acclimatized one day prior to the exposure in the dilution water (M4 medium). After the exposure on day ‘0’, daphnids were observed for immobilisation approximately at ‘24’ and ‘48’ hours and the same was recorded.
No immobilisation was observed in control and daphnids exposed to 0.4 mg/L throughout the experimental period. At the end of ‘24’ hour 3/20, 5/20, 12/20 and 20/20 immobility was observed in daphnids exposed to test concentrations of 0.72, 1.30, 2.33 and 4.20 mg/L respectively. Similarly, at the end of ‘48’ hour 5/20, 8/20, 16/20 and 20/20 cumulative immobilisation was observed in daphnids exposed to test concentrations of 0.72, 1.30, 2.33 and 4.20 mg/L respectively. Thus the percent immobilisation at the end of ‘48’ hour was observed to be 0%, 25%, 40 %, 80% and 100% in daphnids exposed to 0.4, 0.72, 1.30, 2.33 and 4.20 mg/L respectively.
Dissolved oxygen, pH and temperature were measured in bulk of control and test concentrations at the beginning and end of the test in control and in all the replicates of control and test concentrations. Hardness was analyzed once at the start of the experiment in the control.
Dose verification analysis was carried out in the test concentrations of low (0.4 mg/L), intermediate (1.30 mg/L), high concentration (4.20 mg/L) and control (M4 medium) at ‘0’ and ‘48’ hours. The mean recovered active ingredient percentage in the samples were found to be 91.73%, 92.46% and 95.01% at ‘0’ hour whereas, 90.31%, 91.59% and 94.67% at ‘48’ hour for 0.4 mg/L, 1.30 mg/L and 4.20 mg/L concentrations respectively.
CONCLUSION
Based on the test results, the EC50of Potassium Dichromate Pure in Daphnia magna observed for a period of ‘24’ hour was calculated as 1.72 mg/L with upper confidence limits of 2.12 mg/L and lower confident limits of 1.40 mg/L and regression equation Y = 4.0957 + 3.8604 log (x). At ‘48’ hour, the EC50 was calculated as 1.33 mg/L with upper confidence limits of 1.63 mg/L and lower confidence limits of 1.08 mg/L and regression equation Y = 4.5239 + 3.8769 log (x). - Reported statistics and error estimates:
- Hour EC50
(mg/L) LCL
(mg/L) UCL
(mg/L) Regression equation
48 34.58 21.20 60.32 Y = 1.3269 + 2.387 log (x) - Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the test results, the EC50 of FAT 40865/A TE in Daphnia magna observed for a period of 48 hour was calculated as 34.58 mg/L with upper confidence limits of 60.32 mg/L and lower confidence limits of 21.20 mg/L and regression equation Y = 1.3269 + 2.387 log (x).
- Executive summary:
An acute immobilization study in Daphnia magna was conducted in accordance with OECD Guideline 202 with FAT 40865/A TE. A main experiment was conducted with a range of concentrations viz., 2.5, 5.5, 12.1, 26.62, 58.56 and 128.84 mg/L. Forty daphnids with four replicates (10 daphnids in each replicate) for each test concentration and control group was maintained. The test item was formulated with dilution water (M4 Medium). The daphnids with brood chambers were acclimatized one day prior to the exposure in M4 medium. After the exposure on day 0, daphnids were observed for immobilisation at approximately 24 hour and 48 hour and the respective data was recorded. Water Parameters viz.,pH, temperature and dissolved oxygen were recorded at the start and end of the experiment in control and all test concentrations. Hardness was recorded in control at the start of the test. Analytical verification of the test item in the dilution water (M4 medium) samples were performed in the test item concentrations of low (2.5 mg/L), intermediate (26.62 mg/L) and high (128.82 mg/L) at 0 hour and 48 hour. The mean recoveries data are given below:
0 hour – Mean Recovery results: 91.91, 93.19 and 95.75 % for 2.5, 26.62 and 128.84 mg/L concentrations respectively.
48 hours – Mean Recovery results: 90.43, 91.92 and 94.35 % for 2.5, 26.62 and 128.84 mg/L concentrations respectively.
No immobilization was observed in control and daphnia exposed to 2.5 mg/L concentration throughout the experimental period. At the end of 24 hour, no immobilization was recorded in the daphnia exposed to any of the test concentrations except 128.84 mg/L in which 25/40 immobilisation was observed. At the end of 48 hour, 3/40, 5/40, 14/40, 24/40 and 40/40 cumulative immobility were observed in daphnids exposed to 5.5, 12.1, 26.62, 58.56 and 128.84 mg/L respectively.
Thus the percent immobilisation at the end of 48 hours was recorded to be 0, 7.5, 12.5, 35, 60 and 100% in 2.5, 5.5, 12.1, 26.62, 58.56 and 128.84 mg/L respectively.
Based on the test results, the EC50 of FAT 40865/A TE in Daphnia magna observed for a period of 48 hour was calculated as 34.58 mg/L with upper confidence limits of 60.32 mg/L and lower confidence limits of 21.20 mg/L and regression equation Y = 1.3269 + 2.387 log (x).
Reference
IMMOBILISATION - MAIN EXPERIMENT
Concentration (mg/L) |
No. of Daphnids/Replicate |
Immobility |
|||||||||
24 hour |
48 hour (*) |
||||||||||
R1 |
R2 |
R3 |
R4 |
(%) |
R1 |
R2 |
R3 |
R4 |
(%) |
||
Control |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
2.5 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
5.5 |
10 |
0 |
0 |
0 |
0 |
0 |
0 |
1 |
2 |
0 |
7.5 |
12.1 |
10 |
0 |
0 |
0 |
0 |
0 |
1 |
2 |
2 |
0 |
12.5 |
26.62 |
10 |
0 |
0 |
0 |
0 |
0 |
3 |
4 |
3 |
4 |
35 |
58.56 |
10 |
0 |
0 |
0 |
0 |
0 |
6 |
7 |
6 |
5 |
60 |
128.84 |
10 |
5 |
7 |
6 |
7 |
62.5 |
10 |
10 |
10 |
10 |
100 |
Key: mg/L - milligram/liter, No. - Number, R-Replicate, % - percentage
*- Cumulative Immobility
PHYSICO CHEMICAL PARAMETERS – MAINEXPERIMENT
Concentration |
Control |
2.5 mg/L |
5.5 mg/L |
|||
|
0 hour |
48 hour |
0 hour |
48 hour |
0 hour |
48 hour |
pH |
7.5 |
7.8 |
7.5 |
7.7 |
7.4 |
7.6 |
Temperature °C |
21.7 |
21.8 |
22.1 |
22.7 |
22.2 |
22.9 |
Dissolved oxygen (mg/L) |
7.89 |
7.82 |
7.85 |
7.79 |
7.83 |
7.77 |
Hardness* (mg/L) |
213 |
- |
- |
- |
- |
- |
Concentration |
12.1 mg/L |
26.62 mg/L |
58.56 mg/L |
|||
|
0 hour |
48 hour |
0 hour |
48 hour |
0 hour |
48 hour |
pH |
7.4 |
7.6 |
7.4 |
7.6 |
7.4 |
7.6 |
Temperature °C |
22.3 |
22.9 |
22.3 |
23.1 |
22.7 |
23.5 |
Dissolved oxygen (mg/L) |
7.76 |
7.70 |
7.76 |
7.68 |
7.62 |
7.53 |
Concentration |
128.84 mg/L |
- |
- |
|||
|
0 hour |
48 hour |
- |
- |
- |
- |
pH |
7.4 |
7.5 |
- |
- |
- |
- |
Temperature °C |
23.1 |
23.8 |
- |
- |
- |
- |
Dissolved oxygen (mg/L) |
7.68 |
7.60 |
- |
- |
- |
- |
Key: °C – Degree centigrade, mg/L-milligram per liter, * - Analysed at the start of the e
STATISTICAL TABLE
Hour |
EC50 (mg/L) |
LCL (mg/L) |
UCL (mg/L) |
Regression equation |
48 |
34.58 |
21.20 |
60.32 |
Y = 1.3269 + 2.387 log (x) |
LCL – lower confidence limits
Description of key information
The EC50 of FAT 40865/A TE to Daphnia magna observed for a period of 48 hours was calculated as 34.58 mg/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 34.58 mg/L
Additional information
A key study was performed to find out the acute immobilization toxicity in Daphnia magna in accordance with OECD guideline 202 with FAT 40865/A TE. A main experiment was conducted with a range of concentrations viz.,2.5, 5.5, 12.1, 26.62, 58.56 and 128.84 mg/L. Forty daphnids with four replicates (10 daphnids in each replicate) for each test concentration and control group was maintained. The test item was formulated with dilution water (M4 Medium).
No immobilization was observed in control and daphnia exposed to 2.5 mg/L concentration throughout the experimental period. At the end of 24 hour, no immobilization was recorded in the daphnia exposed to any of the test concentrations except 128.84 mg/L in which 25/40 Daphnia were found to be immobilised. At the end of 48 hours, 3/40, 5/40, 14/40, 24/40 and 40/40 cumulative immobility were observed in daphnids exposed to 5.5, 12.1, 26.62, 58.56 and 128.84 mg/L, respectively. Thus the percent immobilisation at the end of 48 hour was recorded to be 0%, 7.5%, 12.5%, 35%, 60% and 100% in 2.5, 5.5, 12.1, 26.62, 58.56 and 128.84 mg/L respectively.
Based on the test results, the EC50 of FAT 40865/A TE in Daphnia magna observed for a period of 48 hour was calculated as 34.58 mg/L with upper confidence limits of 60.32 mg/L and lower confidence limits of 21.20 mg/L and regression equation Y = 1.3269 + 2.387 log (x).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.